UNC-86

WormBase ID: WBGene00006818

WormBase description: Is an ortholog of human POU4F2 (POU class 4 homeobox 2). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II regulatory region sequence-specific DNA binding activity; and transcription factor binding activity. Is involved in mechanosensory behavior; neuron development; and positive regulation of transcription from RNA polymerase II promoter involved in neuron fate specification. Localizes to RNA polymerase II transcription factor complex. Is expressed in neurons. Human ortholog(s) of this gene are implicated in autosomal dominant nonsyndromic deafness 15 and hypopituitarism.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AATCAACGGCAGAAACAGAA-3'

Strain name: SYS68

Cross: OP476 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs476 (unc-86::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): HLH-14, CEH-31, CEH-34, TBX-2, LIN-32, CEH-30, EGL-44, CES-2, EFL-3, HLH-2, CRH-2, ZTF-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-38, DAF-19, EGL-18, ETS-5, HBL-1, K09A11.1, LIM-7, NHR-102, RCOR-1, SEL-8, SWSN-7, HLH-14, EFL-3, CRH-2, AHR-1, AST-1, ATF-7, ATHP-1, BCL-11, CAMT-1, CEBP-1, CEH-20, CEH-39, CEH-43, CEH-44, CEH-48, DAF-12, DAF-3, EFL-1, EGL-46, EGL-5, EGRH-1, FOS-1, GMEB-1, HLH-11, HLH-30, HMG-20, LAG-1, LIN-13, LIN-15B, MDL-1, MEC-3, MEF-2, MEP-1, MLS-2, NFYA-1, NHR-49, NHR-6, POP-1, SDZ-38, SEM-4, SKN-1, SOX-2, SOX-4, SPR-4, T18D3.7, UNC-37, UNC-62, Y55F3AM.14, Y73E7A.1, ZAG-1, ZTF-6

Targeted by: CEH-31, LSY-2, NFYA-1, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: DAF-19, ETS-5, RCOR-1, SWSN-7, EFL-3, CRH-2, AHR-1, AST-1, ATF-7, ATHP-1, CAMT-1, CEBP-1, CEH-20, CEH-39, CEH-5, CEH-74, DAF-3, EFL-1, EGL-46, EGRH-1, GMEB-1, HLH-30, HMG-20, K10B3.5, LAG-1, LIN-13, LIN-15B, MDL-1, MEC-3, MEF-2, MEP-1, NFYA-1, NFYC-1, NHR-49, POP-1, SEM-4, SKN-1, SOX-2, SPR-4, SVH-5, T18D3.7, UNC-37, UNC-62, Y73E7A.1

Targeted by: CEH-31, LSY-2, NFYA-1, ZIP-8