CEH-30

WormBase ID: WBGene00000451

WormBase description: Is an ortholog of human BARHL1 (BarH like homeobox 1) and BARHL2 (BarH like homeobox 2). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in negative regulation of apoptotic process; nervous system development; and sex differentiation. Localizes to nucleus. Is expressed in embryonic cell and neurons.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AAATGTCACTTCTCGACCCT

sgRNA2: AGTCGGATCCAGATAAAAAG

5'HR: GCATGCGAAACTATAAACAGAACTAATTTGAGAAAAGCGACAAAAATATGTATTCATTCAGAAAAAAAGGAAACAGAGTGTTTACAACAATGATTGAAATTATTTGAAATTAAATAAAAAACAATCACCGTATTTCTACAGGTTACTGAAATCGTGATTCGTAACAAAAATTTACCGTAACTTCTATAACTTTTTTTTGCCGAAAAATACGTTGAGTAACCTTTTGTCGATTTTGTGAAAAACAAACCGACTAAGCTTATATATGGAGATTAGTAAGATGGTCTTTCTGCAAGTAAGTGAAAAACTGTGAAAATGAAACTTTAACATTTCGAGCACCAACCTGAAAATTAGAGTTCGATATCCAGCTTTGGCAGAATAATATTCCATATTGTTTATTTCCAAGTTTGACAATTCATCAAATGTTTCCCTAGAACCACCGTTTGACAGGTTGCTCCAATTAGCCGCAGTGTGCAGCTATTCCCAACCATCTTCCCCCATCTCATTTTCGCCATCATTTTCCCCCGCATCATCTGAGATGTGCAAAATGAAAGGTCAAGTTATAGATTTCGTGTTATTAAATACAAATAATTGACTTCTCATGTGGCGTTGGATCTCGAAAATCGAGTGTAATTGTCATGACCATGTTTAGGAGGGCGGTGGTGGTAGCTGCTGCTGCATCATCCTTCCCAATGTCAACGCACCCTCTTCTCTCACCCAAATTGCCAGGCCGTCGCCCCGCCCACTCTCGCACATTTAACTTGTCTTATAGTCACCCCTCATCGTCCGACAATAACCAATCATCGGTGCCTTCAACAGTGTCGCATTCCCGACTTTCGAACGTGCAAAGCATTCGTTTAACACTTTTCTACAGTACCACTCACTTCCCAAAACC GGT

3'HR: CTGCAGATGTCACTTCTCGATCCTCGACAGTTTCTGCTTCCTGCTTTTTATCTAGATCCGACTACACAAGCACTTCTAGCTCAAGCAGCTTCAACTTCCCCGTGCAACAAAATTTCGTCTTCTTCTTCATTCCGCATTTCTGACATTCTCGAGCAATCCCCAAACAATTCATCCCATTCAAACGATCACGGTAAGTCATTACACCTGCAATTGATTCCAAAATTCCAAACATTCAAAACGACAGCCATTAAAAGTTTTGTGCGCGGCGCGTTACCCATCAAAAATTTACTCAACCATGCTCTCGATTTTCTCCTCTCAATGGTGACTACCCGGAAATGATAGTTTGAATGTAGGGGGGGGGGGGGGTAAAAGGATTAATGCCTTAATGAACATATGAATACAGTTCTCTTCCGGGGTCAATCTTTTGTTTTGTTCTTTTTGTGCATCAATAACCATCAACTTTCAGACCCATCGCCACAATCAATTAAATCTGATTTTTCAACTTCGCCAAGAGCAAGCAGCCCCGGCGGCGATCGAATGGGTAGTCCAGGCAGTTGCAAAAAATCTCGGAAGGCAAGAACCATCTTCACTGACAAGCAGTTGCAAGAGTTGGAGAATACATTTGAAAAGCAAAAGTATCTATCAGTCCAGGACAGGATGGATTTGGCGCACAGAATGGGTCTCACTGATACTCAAGTCAAGACGTGGTACCAAAATAGAAGGTTAGTAATAACGTCTTTCAATTATCGTTGGAATTATCGTTGGAATTATCGTTGGTAGCAGAGTCAATTCTTAACATACATATGTACATTCTCAAATTAATAACAAGCGTAGCCGCCAATTGAGCCCACCCTTGTAGATTACTACACCAACCACGCTCACATTTCCTAATTATTGTTCAAGGCGGCGGTGTTCTATGTGAATAACTAGT

KI verification primers: CEH-30-NKIF: 5'-TAATATCGGCAAGTGGCCTTATTC-3' CEH-30-NKIR: 5'-AGATTGTGAATGCATGGATACATTTC-3'

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Strain name: SYS744

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-30 (devKi253 [mNeonGreen::ceh-30]) X

Expression pattern: Lineage-specific, Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIM-6, CEH-31, UNC-42, PAG-3, SEM-2, LIN-11, UNC-86, EGL-46, LIN-32, VAB-3, CEH-48, VAB-15, ZAG-1, EGL-44, SOX-2, HLH-30, NGN-1, ELT-6, CEH-43, CES-2, ZTF-11, EFL-3, HLH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: REF-2, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ZIP-8