WormBase ID: WBGene00006870
WormBase description: Is an ortholog of human PAX6 (paired box 6). Is predicted to have DNA-binding transcription factor activity. Is involved in several processes, including anterior/posterior pattern specification; nematode male tail tip morphogenesis; and positive regulation of transcription by RNA polymerase II. Localizes to cytoplasm and nucleus. Is expressed in several structures, including anterior ganglion (post); distal tip cell; head muscle; neurons; and somatic nervous system. Human ortholog(s) of this gene are implicated in several diseases, including cataract 11 multiple types; diabetes mellitus (multiple); and isolated microphthalmia 3.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TTTAGAACTATTCTCTACTG
sgRNA2: ATTTGTGAGTGAGTCAGAGA
5'HR: GCATGCCTGGGCTCAGTCAGCCAGCAATGGATCCATATTCGTAAGTTATAGATCAAACCATCATCAACGGTTATGAACTATTTATAACTTTATTAGACTAAAATAAAATTGTTTTTGTAGTCAAATATAATATAATCTACACCATTCAACAATCAATGGAAATTTCTCAATATTTCAGATTTGGAATTGCCAATGGTTTTTCAATGGCTCCATATCCACAAGTAACAGATTTCCAACCACATCACATGTTCCAAGGAAGATCGCCGTATGATTTCCCATATCCAAGAATGCCGACCAATGGGCATGGTTTCCAACAATCAATGAGTCCGGCAACAACGGCGGTAGGAGGTAATAAATGATTGTTGATTTTGATTTCTAATTCTTTTGCGCAAAAACCTATTAACATGATTTCAATTGATTTAGAAGTGTTAAAACGCGAAAGAGAGGTGTAGGTCTCGGGAGTACTTTTTATAATGTGTTGAAAGATGTCAGTTTTTTATGAAGGCTGCAAGCTTGAATTTTTTAGGCTTTTTCGGGCACTAATCCTCAAACTCAGTATTTCTAATAAATTCCGATAGGCTACCGTAACCTCCAACACTGTCTTCTTCAATTCTCCAGCCATGTACTGTGTCGGCCTTCGTTCCCTCACTATTTCTTTGTCTTTTGTGTGACATATGCCTCTGTTGGCTGTCACCTCACATTTATGTCCAATACAATATGCGTTGGGATCAGCACGATACGTTTACCTATACATTTTTTTTCTGATGCAGCGCGCACTCAGATGGGTAATGGGTGGGAGTGTGAGATAATTTTGAAGGGATATGATCTTTTGGGATAATCAGTTGCGTATTTTCAGACATTCCGACACTTTCTTCTGGAATGAGCCTACCGGTTTCTGCCGTGCTCAATTCCATTGACCCGTCCCTCACCCATTCACAAATGCATGAACTCAGCGACCTCACACAAGAACATTATTGGAGACCTCAGGGTACC
3'HR: CTGCAGAGAATAGTTCTAAAGCAGATTGAATATATATATCCCTATTAAATTTTAAGACATTAATTTCAGTTTGTCTACCATTTTTTTGTCATTCCCTTTAATTCCAGTTCCAGTCCACATCATCATCTCGTTCTTTTCTTTTGGTTCAATTTCTTTTCCTGGCCATAAACTTCTGGGTTCATCTGAATTTCTCAAATCAATTATGAAATCAAAGTCTAATCATGGTTTCATTTGTCACTTTTTAAATGTTAACTCAATTAATTTTTAATAGAATCAATGCAAAACTTTCTATGAGCTTTTTATATTATTTCACCCCGTGGTCCCTTTTTCCTTCATTCATGAGCTGTCACATATTCAATCCCCATTCCTTTATGCTAGATTTTGTAGACCAGTGACTTTTGTTGTCCCATTTAAATGGAACATGTAGAGTATTGCTCTACAGAATATCGTGCACAAAATGACATTTGCCCCACGTCCCCCCCCCCCCCTCTCCTTAAATTTCAAACTTCTTCCAGAGTTTTCAATTGTATCACATATTTTTTATTCTGTTGAGCATGAAAATTGTTTTTCCGTTTTCAAACTATATATTCTTAGGTTCAACTATACAATTCAATTGATTATGACATATTGAAAACGAAAAAAAACTGTTGTTATCATTAACTCTGATTTGTCAGTATTTTCTCAATTTTTTAAACCTTAGAAATCTACCCACTTTCCATGTAGAGTGTCAATTAAATTTTATCTTGGAGTGAGCGATTTTGGAACTTATAGTGAAAAATGAAAAAGTTTCAATTGTTTCATATCATGCATTCTATCTGGTTGTTTTCTTTTTTTCCAAATTTTGACCATTTCCTTCCTCTCTTGAACCGTTCTCTCATTGTTATATTTGTCGAAAAGTAAAATACCTAATGTAATAAAATGTGTACTCTTATAAAAATTCTTACTAGT
KI verification primers: VAB-3-CKIF: 5'-TAACATGACAAATCCGATTGGATC-3' VAB-3-CKIR: 5'-TCTTCCAGTACCAATTGTATCTTTATTTC-3' Download
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Strain name: SYS633 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; vab-3 (devKi190 [vab-3::mNeonGreen]) X
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Expression pattern: Lineage-specific, Neuronal-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-31, UNC-3, CND-1, UNC-30, Y56A3A.28, UNC-42, UNC-130, CEH-30, EGL-46, LIN-32, CEH-48, ZAG-1, HLH-30, ALR-1, LIN-26, ATF-7, BAZ-2, SOX-2, EGL-44, HAM-2, EFL-3, NGN-1, ZIP-8, CEH-43, SPTF-1, MLS-2, ELT-6, NHR-25, CRH-2, HLH-2, NFI-1, ELT-1, ZTF-11, CEH-36
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CND-1, HAM-2, ZIP-8, CEH-43, SPTF-1, MLS-2, ELT-1, CEH-36, LSY-2, NFYA-1, TBX-2, UNC-62, UNC-130, BLMP-1, CEH-8, EGL-13, ELT-3, FKH-8 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CND-1, HAM-2, ZIP-8, ELT-1, LSY-2, NFYA-1, CEH-8, EGL-13, FKH-8
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