HLH-2

WormBase ID: WBGene00001949

WormBase description: Is an ortholog of human TCF12 (transcription factor 12); TCF3 (transcription factor 3); and TCF4 (transcription factor 4). Exhibits several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; bHLH transcription factor binding activity; and protein dimerization activity. Contributes to RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including generation of neurons; gonad morphogenesis; and nematode male tail tip morphogenesis. Localizes to RNA polymerase II transcription factor complex and cytoplasm. Is expressed in several structures, including distal tip cell; lateral ganglion; non-striated muscle; ray precursor cell; and terminal bulb. Human ortholog(s) of this gene are implicated in Fuchs' endothelial dystrophy; Pitt-Hopkins syndrome; and craniosynostosis.

Reporter verification: Verified by comparing expression pattern to the literature (Krause et al., 1997)
Primers used to verify fosmid reporter: nan

Strain name: SYS1049

Cross: OH12412 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; otIs502 (hlh-2(fosmid)::YFP + myo-3p::mCherry)

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, TTX-3, UNC-3, HLH-14, CND-1, LIN-32, ZAG-1, UNC-42, NGN-1, CEH-48, UNC-86, LIN-11, CEH-31, TAB-1, HLH-3, UNC-30, CEH-32, EGL-46, PROS-1, MBF-1, EGL-44, NHR-67, HLH-30, VAB-3, ZTF-11, CEH-30, COG-1, SOX-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CND-1, UNC-42, CEH-31, HLH-30, COG-1, CEH-34, CEH-36, CEH-39, ELT-1, GMEB-2, HAM-2, HLH-8, HMG-4, LIN-13, M03D4.4, MLS-2, NFYA-1, PHA-2, PHA-4, REF-2, SPTF-1, UNC-62, ZIP-8, BLMP-1, DPL-1, DSC-1, EFL-1, FKH-4, FKH-8, LSY-2, MEP-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CND-1, UNC-42, CEH-31, HLH-30, COG-1, CEH-34, CEH-36, CEH-39, ELT-1, GMEB-2, HAM-2, HMG-4, LIN-13, M03D4.4, MLS-2, NFYA-1, PHA-2, PHA-4, REF-2, SPTF-1, EFL-1, FKH-8