ZTF-11

WormBase ID: WBGene00009939

WormBase description: Is an ortholog of human MYT1L (myelin transcription factor 1 like). Is predicted to have RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in several processes, including generation of neurons; nematode larval development; and positive regulation of transdifferentiation. Localizes to nucleus. Is expressed in g1 and head neurons. Human ortholog(s) of this gene are implicated in autosomal dominant non-syndromic intellectual disability 39.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AATGCGAGTCACCTGTTCCA-3'

Strain name: SYS99

Cross: OP511 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs511 (ztf-11::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, UNC-3, EGL-13, CFI-1, HLH-14, UNC-86, EGL-46, CEH-48, UNC-42, CEH-8, LIN-32, NGN-1, CEH-30, LIN-11, CND-1, ZAG-1, TTX-3, NOB-1, UNC-30, CEH-32, COG-1, PAG-3, SPTF-1, CEH-31, CEH-6, SOX-2, EGL-44, HLH-2, HLH-3, VAB-3, Y73E7A.1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: EGL-46, ZAG-1, CEBP-1, CEH-20, CEH-38, CEH-44, CRH-2, DAF-19, DUXL-1, EFL-1, ETS-5, GMEB-1, HBL-1, LIM-7, MDL-1, MEP-1, NHR-102, NHR-49, POP-1, SWSN-7, SYD-9, ZFH-2, ZTF-6, EGL-13, HLH-14, LIN-32, Y73E7A.1, AHR-1, ATHP-1, DAF-12, EFL-3, EGL-18, EGRH-1, HLH-11, HLH-30, LAG-1, LIN-1, LIN-13, LIN-15B, LIN-39, NFI-1, RCOR-1, SDZ-38, SEL-8, SEM-4, SMA-2, UNC-37, UNC-62

Targeted by: LIN-32, CND-1, CEH-31, CEH-83, FKH-6, HLH-1, HMG-4, MLS-2, NFYA-1, TBX-2, UNC-120, UNC-55, UNC-62, ZIP-8, BLMP-1, DSC-1, FKH-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEBP-1, CEH-20, CRH-2, DAF-19, DUXL-1, EFL-1, ETS-5, GMEB-1, MDL-1, MEP-1, NHR-49, POP-1, SWSN-7, SYD-9, EGL-46, LIN-32, Y73E7A.1, AHR-1, ATHP-1, F19F10.9, HLH-30, K10B3.5, LAG-1, LIN-13, LIN-15B, LIN-39, NFI-1, NFYC-1, RCOR-1, SMA-2, UNC-37, UNC-62, ZTF-6

Targeted by: LIN-32, CND-1, CEH-31, CEH-83, FKH-6, HMG-4, MLS-2, TBX-2