SPTF-1

WormBase ID: WBGene00009743

WormBase description: Is an ortholog of human SP7 (Sp7 transcription factor). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is expressed in ASJ; epithelial cell; intestinal cell; and pharyngeal cell. Human ortholog(s) of this gene are implicated in osteogenesis imperfecta type 12.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GCACGTAAGCGAGGAGTTCA-3'

Strain name: SYS112

Cross: OP707 x RW10226

Strain genotype: itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR; unc-119(+)); stIs10226 (his-72p::his-24::mCherry::let-858 3' UTR; unc-119(+)); wgIs707 (sptf-1::TY1::EGFP::3xFLAG + unc-119(+)) II

Expression pattern: Lineage-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): EGL-13, LIM-4, CEH-8, MBF-1, RNT-1, UNC-42, Y73E7A.1, CEH-32, CND-1, FKH-2, Y56A3A.28, CEH-43, CEH-48, UNC-39, CEH-16, TTX-1, CEH-45, LIN-32, NHR-23, LIN-1, CEH-41, SOX-2, LIN-26, VAB-3, ZTF-11, HLH-3, HLH-30


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: Y73E7A.1, CEH-43, CEH-48, UNC-39, TTX-1, LIN-1, VAB-3, HLH-30, AST-1, ATF-7, BCL-11, BED-3, BLMP-1, CEBP-1, CEH-2, CEH-20, CEH-38, CEH-39, CEH-44, CES-2, CRH-2, DAF-19, DAF-3, DMD-5, EFL-1, EFL-3, EGL-18, EGL-44, EGL-46, ETS-5, FAX-1, GMEB-1, GMEB-2, HAM-2, HBL-1, HLH-1, HLH-2, LAG-1, LIM-7, LIN-11, LIN-13, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NHR-102, NHR-49, NHR-6, PHA-4, POP-1, PROS-1, RCOR-1, SDZ-38, SEL-8, SKN-1, SWSN-7, SYD-9, T07F8.4, UNC-130, UNC-37, UNC-62, Y55F3AM.14, Y75B8A.6, ZAG-1, ZFH-2, ZTF-6

Targeted by: HLH-1, LSY-2, NFYA-1, UNC-62, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: Y73E7A.1, CEH-43, CEH-48, TTX-1, HLH-30, AST-1, BED-3, BLMP-1, CEBP-1, CEH-2, CEH-20, CEH-39, CES-2, CRH-2, DAF-19, DMD-5, EFL-1, EFL-3, EGL-44, ETS-5, FAX-1, GMEB-1, GMEB-2, HAM-2, HBL-1, HLH-1, HLH-2, LAG-1, LIN-13, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NHR-49, PHA-4, POP-1, PROS-1, RCOR-1, SKN-1, SWSN-7, SYD-9, T07F8.4, UNC-37, UNC-62, Y75B8A.6, ZAG-1, ZFH-2

Targeted by: LSY-2, NFYA-1