Single-Cell Protein Expression Atlas of Transcription Factors

LSY-2

WormBase description: Is an ortholog of several human genes including AC008481.3; ZBTB32 (zinc finger and BTB domain containing 32); and ZFP91-CNTF (ZFP91-CNTF readthrough (NMD candidate)). Is predicted to have DNA-binding transcription factor activity and transcription regulatory region DNA binding activity. Is expressed in several structures, including ASE; head muscle; head neurons; intestine; and somatic nervous system. Human ortholog(s) of this gene are implicated in autosomal dominant non-syndromic intellectual disability 22.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGTAAGCTCCCAAAATCGAGCA-3'

Strain name: SYS105

Cross: OP240 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs240 (lsy-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, ATFS-1, ATHP-1, BLMP-1, CAMT-1, CEBP-1, CEH-13, CEH-16, CEH-2, CEH-20, CEH-32, CEH-38, CEH-39, CEH-43, CEH-45, CEH-6, CFI-1, CRH-2, DAF-19, DVE-1, EFL-1, EGL-18, EGL-46, EGL-5, EGRH-1, EOR-1, ETS-5, FOS-1, HLH-1, HLH-11, HMG-20, HMG-3, K09A11.1, LET-381, LIM-7, LIN-11, LIN-13, LIN-39, MAB-9, MDL-1, MEF-2, MEP-1, NFI-1, NGN-1, NHR-10, NHR-102, NHR-23, NHR-25, NHR-49, NHR-67, NOB-1, PAL-1, PAX-3, PHA-4, POP-1, PROS-1, RCOR-1, RNT-1, SEM-4, SOX-2, SPTF-1, SWSN-7, T07F8.4, TBX-2, TTX-1, UNC-120, UNC-130, UNC-3, UNC-30, UNC-37, UNC-42, UNC-62, UNC-86, VAB-15, VAB-3, VAB-7, Y73E7A.1, ZAG-1, ZFH-2, ALR-1, APTF-1, ARID-1, AST-1, ATF-7, BCL-11, CEH-18, CEH-22, CEH-23, CEH-34, CEH-36, CEH-44, CEH-48, CEH-8, CEH-88, DAF-12, DAF-3, DMD-5, DNJ-17, DPFF-1, DUXL-1, EGL-13, EGL-44, ELT-1, ELT-2, ELT-3, FKH-10, GMEB-1, HAM-2, HBL-1, HLH-2, HLH-30, IRX-1, KLF-3, LAG-1, LIN-1, LIN-15B, LIN-22, M03D4.4, MML-1, NFYA-1, NHR-186, NHR-35, NHR-6, NHR-85, REF-2, SDZ-38, SEL-8, SKN-1, SMA-2, SPR-4, TAG-68, TBX-36, TLP-1, Y55F3AM.14, Y75B8A.6, ZIP-3, ZTF-6

Targeted by: CEH-18, CEH-34, CEH-39, CND-1, ELT-2, HLH-1, HLH-30, LIN-13, MEP-1, NFYA-1, PAX-1, PHA-4, RNT-1, SPTF-1, TBX-2, UNC-42, UNC-55, BLMP-1, C16A3.4, CEH-82, DPL-1, EFL-1, ELT-3, MDL-1, SKN-1, SWSN-7, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, ATFS-1, ATHP-1, BLMP-1, CAMT-1, CEBP-1, CEH-13, CEH-16, CEH-2, CEH-20, CEH-32, CEH-39, CEH-43, CEH-45, CEH-6, CRH-2, DAF-19, DPL-1, DVE-1, EFL-1, EGL-46, EGL-5, EOR-1, ETS-5, HLH-1, HMG-20, HMG-3, K10B3.5, LET-381, LIN-11, LIN-13, LIN-39, MDL-1, MEF-2, MEP-1, NFI-1, NFYC-1, NHR-10, NHR-23, NHR-25, NHR-49, NHR-67, NOB-1, PAL-1, PAX-3, PHA-4, POP-1, PROS-1, RCOR-1, SEM-4, SOX-2, SPTF-1, SWSN-7, T07F8.4, TBX-2, TTX-1, UNC-130, UNC-30, UNC-37, UNC-42, UNC-62, UNC-86, VAB-15, VAB-3, VAB-7, Y73E7A.1, ZAG-1, ZFH-2, APTF-1, ARID-1, ATF-7, BCL-11, CEH-22, CEH-23, CEH-5, CEH-8, CEH-88, CFI-1, DAF-3, DMD-5, DPFF-1, DUXL-1, EGL-44, ELT-1, ELT-2, ELT-3, GMEB-1, HAM-2, HBL-1, HLH-11, HLH-30, IRX-1, KLF-3, LAG-1, LIN-15B, LIN-22, M03D4.4, MAB-9, MML-1, NFYA-1, NHR-186, NHR-35, NHR-85, REF-2, RNT-1, SKN-1, SMA-2, SPR-4, SVH-5, TBX-36, TLP-1, UNC-120, ZIP-3