ELT-3

WormBase ID: WBGene00001251

WormBase description: Is an ortholog of several human genes including GATA1 (GATA binding protein 1); GATA4 (GATA binding protein 4); and ZGLP1 (zinc finger GATA like protein 1). Exhibits RNA polymerase II regulatory region DNA binding activity. Is involved in determination of adult lifespan; positive regulation of transcription from RNA polymerase II promoter in response to increased salt; and positive regulation of transcription from RNA polymerase II promoter involved in defense response to fungus. Localizes to nucleus. Is expressed in several structures, including Caaaaa; Caaaap; hermaphrodite distal tip cell; hypodermal cell; and rectum. Human ortholog(s) of this gene are implicated in several diseases, including Down syndrome; congenital heart disease (multiple); and hypoparathyroidism-deafness-renal disease syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GTGATAGTCTCAGGCACCTTATTGA-3'

Strain name: SYS111

Cross: OP559 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs559 (elt-3::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Skin-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NHR-102, NHR-12, NHR-23, LIN-26, TBX-9, PAL-1, HAM-2, ELT-1, NHR-25, T07F8.4, HBL-1, UNC-62, CEH-20, TBX-8, IRX-1, F23B12.7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-23, ATFS-1, BED-3, CEBP-1, CRH-2, EFL-1, ETS-4, ETS-5, FOS-1, LIM-7, MDL-1, MML-1, NHR-49, SKN-1, ZTF-6, NHR-102, LIN-26, NHR-25, HBL-1, UNC-62, CEH-20, ALR-1, ARID-1, ATF-7, ATHP-1, BCL-11, BLMP-1, CAMT-1, CEH-13, CEH-18, CEH-38, CEH-45, DAF-19, DMD-5, DNJ-17, EGL-13, EGL-18, EGL-44, EGRH-1, EOR-1, FKH-2, GEI-3, GMEB-1, GMEB-2, HLH-11, HLH-30, HMBX-1, HMG-20, KLF-3, LAG-1, LIN-1, LIN-13, LIN-15B, LSY-2, MEF-2, MEP-1, NFI-1, NFYA-1, NHR-10, NHR-35, NHR-85, NOB-1, RCOR-1, SEM-4, SMA-2, SWSN-7, TLP-1, UNC-3, VAB-3, Y73E7A.1, Y75B8A.6, ZIP-3, ZTF-30

Targeted by: UNC-62, ELT-2, NFYA-1, REF-2, ZIP-8, IRX-1, BLMP-1, CEH-8, LSY-2, NHR-85, PQM-1, TBX-2, TLP-1, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-23, AHA-1, ATFS-1, CEBP-1, CRH-2, EFL-1, ETS-5, MDL-1, MML-1, NHR-49, SKN-1, LIN-26, NHR-25, HBL-1, UNC-62, CEH-20, F23B12.7, ALR-1, ARID-1, ATF-7, ATHP-1, BCL-11, BED-3, BLMP-1, CEH-5, DAF-19, DMD-5, DPL-1, EGL-44, EOR-1, F19F10.9, FKH-6, GEI-3, GMEB-1, HLH-30, HMG-20, K10B3.5, KLF-3, LAG-1, LIN-1, LIN-13, LIN-15B, LSY-2, MEF-2, MEP-1, NFI-1, NFYA-1, NFYC-1, NHR-10, NHR-35, NHR-85, NOB-1, RCOR-1, SMA-2, SVH-5, SWSN-7, TLP-1, Y73E7A.1, ZIP-3, ZTF-30, ZTF-6

Targeted by: UNC-62, ELT-2, NFYA-1, REF-2, ZIP-8, IRX-1, BLMP-1, LSY-2, NHR-85, PQM-1, TBX-2, TLP-1, UNC-130