WormBase ID: WBGene00003779
WormBase description: Is an ortholog of human HOXD12 (homeobox D12). Exhibits sequence-specific DNA binding activity. Is involved in several processes, including embryonic body morphogenesis; nematode male tail tip morphogenesis; and positive regulation of nematode male tail tip morphogenesis. Is expressed in head muscle; intestine; linker cell; somatic nervous system; and tail.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: GCTCATATATGGAATGATTT
sgRNA2: TCCAACAAACTCCATTCTTC
NOB-1-NKIsg3: GAATGGAGTTTGTTGGACAG
5'HR: GGATCCTCCCTTGCTTTTTGCACTCAACTTGTCTCTCCTATTCATTCTCTGTTCCCCGCCCGCCCCGCTCCTCGCCGCCCAATTCGTCAGAGGGGTACGGTAGACAAGTGGGTGGAGCGTGCGTGTTATGTAGTGTCCTCCTTTCTGCTCCTCGGCCTCATCACCTTTCACGGCCCCCCGCCCCTTATTCTCTTTATAGTCTCTTTGGTGTTTTCCAACACAAGGGGGAAAGAGGAAACACCTTTTTAATGCGTTTATAAAACTTTTTTAGTGCTCTCTTAATCTTTATTACTTGTCTCGCATGCATGTTTCAACATTTATGTTATTTATGTGCCCCCCCAGCTTTTATTTTATTACCCTCAAAAGGTTTATGAGGTTTAAAATTATTTTATTGGTGCTTAAGGTTTTGATTGCCGCGTTTGTCGCCGATAAATAATAATTACTGTTATAAGCTTATTTACTAATTAATTAATTTTCCGCGTCAAAATCCTAAATATATCGTAGCTAAAGCTTTATATCTAGTTAGTATGGCCCCCCATCAATCGACTTTGTCGTCTTATATTTCAGCGGTTTCATAAGATTTTTGATACCTTCAAAAACCAGAGCATAACTATAGATTTGAAAAAGATATTGTCGTTCGAGTTTTTGACATATGATCAAAAAGAACCGAGATATAAGCGTTCAAAGTCGAGTGAGGGGGGCCGTACTAATAGGAAATATACGGTAAATGACTACTGTAGCTCTTACAAGCCGTGCTAAATTCATTTCAAAAATCGAGCCCGTAAATCGACACAAGTGCTACAGTAGTGTTCAAATATAAGCTTTTTAAATTCTCGTTTTTCTTTTCCTTACTTTTTCAAGCTCAATCTCGCAAAGTGCTCAACTTTCAGGTTCAAAAACAGATCTCCAAACCCAAATAACTCCCCAAGCTGCTCATATATGGAGGTACC
3'HR: CTGCAGATGATTTCGGTGATGCAACAAATGATTAATAACGACTCTCCAGAAGACTCTAAAGAATCTATCACCTCTGTCCAACAGACCCCGTTTTTCTGGCCAAGTGCTGCTGCTGCCATACCGTCGATACAAGGAGAAAGCCGTTCGGAAAGAGAATCTGAAACTGGGTCGAGTCCACAGCTAGCGCCGTCTAGGTTAGTGTGAAGTTGATTTGGCAAATGACATCTATCATTTTCAGCACAGGAATGGTTATGCCTGGAACAGCAGGGATGTATGGGTTCGGACCAAGTCGAATGCCAACTGCCAACGAGTTTGGAATGATGATGAACCCGGTCTACACGGATTTCTATCAAAATCCGCTGGCCTCAACTGGTTGGTACTCATACGGACAACCGTATCAATTCACAGCTAACTACTCCATTCCAAGTCTCGATGGAAATTTAAGTGGTTAGTTCCAACATCTCACTTCTATTATGTCATTGTGCTCTTCCATCTGCCCGCCCACCTCCGCATTCCTCGCTATCACTAGCTTTGATCTTACCGCCGCGAGTTGCAAGGGTAGGAGAACACGGTTTTCTGCTCCGTAACTCGGAACTTTCTAAAAGATCATAAGTCAATTCGCAGCCTATTTGGATCTCATTCCTTTCCTTTTCCGCATAACAATCATAAAATTATTAATTACCGTTGAGACCTCCCCCACCCATAAATTTCGCCTCGCCCTTGCCCAATTAGTAATCGATATCAAATCACACACGCGCCTTCAATTCCTACCGTACCACTTTATCTTCCGATCATCTCTTTTTTTGTTCGTCATTCTTCCTTATATAGAATCTTCTTCATTTTTTTGCCCCCATACAGTAATCGGAGAGTGCACTCTCCTCGCGGCGCCAGCGGCGACCCTTTCAAGTTGTATATGTGTGTGTGTGTGTATCGACGACGTGTATAACTAGT
KI verification primers: NOB-1-NKIF: 5'-CGCTCAAATTTTGTATCATATATATGG-3' NOB-1-NKIR: 5'-AAACTGATGTAAAGTGTCTGTTATCGA-3' Download
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Strain name: SYS724 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; nob-1 (devKi231 [mNeonGreen::nob-1]) III
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): UNC-3, VAB-15, PHP-3, COG-1, NHR-23, NHR-12, REF-1, NHR-25, HLH-14, LIN-26, GMEB-1, LIN-1, VAB-7, ELT-1, BAZ-2, LIN-32, TLP-1, HAM-2, HBL-1, Y75B8A.6, NFI-1, F23B12.7, CEH-74, ZTF-11, CEH-43
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: COG-1, CEH-14, CEH-36, CEH-83, ELT-2, LSY-2, NFYA-1, PHA-4, TBX-2, ZIP-8, BLMP-1, DSC-1, ELT-3 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: COG-1, CEH-14, CEH-36, CEH-83, ELT-2, LSY-2, NFYA-1, PHA-4, TBX-2, ZIP-8, BLMP-1, DSC-1, ELT-3
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