TLP-1

WormBase ID: WBGene00006580

WormBase description: Is an ortholog of human ZNF503 (zinc finger protein 503) and ZNF703 (zinc finger protein 703). Is predicted to have repressing transcription factor binding activity. Is involved in positive regulation of transcription by RNA polymerase II. Is expressed in several structures, including body wall musculature; rectal valve cell; somatic nervous system; tail precursor cell; and tail spike.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TCCGCATCTTCTGAACAATC-3'

Strain name: SYS107

Cross: OP321 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgEx321 (tlp-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Muscle-specific, Transient-expression

Lineage expression pattern

350 stage



600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): EGL-5, SYD-9, VAB-7, SNAI-1, RNT-1, LIN-22, PHP-3, LIN-39, VAB-15, NOB-1, HLH-1, CEH-40, CEH-20, UNC-120, PAL-1, NHR-23, UNC-62, NHR-12, CEH-16, GMEB-1, COG-1, LIN-26, M03D4.4, HMBX-1, UNC-30, HBL-1, TBX-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-62, CRH-2, ELT-3, ETS-4, ETS-5, MDL-1, Y55F3AM.14

Targeted by: RNT-1, HLH-1, UNC-120, UNC-62, COG-1, M03D4.4, ALY-1, CEH-18, CND-1, ELT-1, ELT-2, NFYA-1, PQM-1, TBX-2, TBX-8, UNC-55, LIN-39, VAB-15, BLMP-1, CEH-82, DPL-1, EFL-1, EFL-3, ELT-3, IRX-1, LIN-13, LSY-2, MDL-1, MEP-1, NHR-85, PAX-3, SWSN-7, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-62, CRH-2, ELT-3, ETS-4, ETS-5

Targeted by: RNT-1, HLH-1, UNC-120, UNC-62, COG-1, ALY-1, CEH-18, CND-1, ELT-1, NFYA-1, TBX-2, TBX-8, UNC-55, LIN-39, VAB-15, BLMP-1, CEH-82, DPL-1, EFL-1, EFL-3, ELT-2, ELT-3, IRX-1, LIN-13, LSY-2, MDL-1, MEP-1, NHR-85, SWSN-7, UNC-130