UNC-130

WormBase ID: WBGene00006853

WormBase description: Is an ortholog of human FOXD4 (forkhead box D4). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity and transcription corepressor activity. Is involved in several processes, including axon extension involved in axon guidance; negative regulation of transcription by RNA polymerase II; and nematode male tail tip morphogenesis. Localizes to nucleus. Is expressed in several structures, including HO neuron; hypodermis; ray; tail; and ventral body wall muscle. Human ortholog(s) of this gene are implicated in cataract 34 multiple types; congenital aphakia; and persistent fetal circulation syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GATTCGTCACAACCTAAGCCTCAA-3'

Strain name: RW11144

Strain genotype: unc-119 (ed3) III; itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); wgIs76 (unc-130::TY1::eGFP::3xFLAG(P000007_E01))

Expression pattern: Lineage-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NHR-35, GMEB-1, TTX-1, BAZ-2, VAB-3, ALR-1, LIN-26, Y56A3A.28, NHR-25, HAM-2, ELT-1, TBX-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-35, VAB-3, ALR-1, ATF-7, ATFS-1, ATHP-1, BCL-11, CEH-20, CEH-38, CRH-2, DAF-12, DMD-5, DPFF-1, EFL-1, EGL-44, EGRH-1, ELT-3, ETS-5, FOS-1, GEI-3, HBL-1, HLH-1, HLH-11, HLH-30, LAG-1, LIM-7, LIN-13, LIN-15B, LIN-39, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NHR-102, NHR-49, NHR-85, RCOR-1, RNT-1, SDZ-38, SKN-1, SMA-2, SNAI-1, TLP-1, UNC-120, UNC-62, Y55F3AM.14

Targeted by: HAM-2, ELT-1, BLMP-1, CEH-36, CEH-43, CEH-83, ELT-2, HLH-1, LSY-2, NFYA-1, SPTF-1, TBX-2, UNC-120, UNC-55, UNC-62, ZIP-8, RNT-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-35, ATF-7, ATFS-1, ATHP-1, CEH-20, CEH-5, CRH-2, DMD-5, DPFF-1, DPL-1, EFL-1, EGL-44, ELT-3, ETS-5, GEI-3, HBL-1, HLH-1, HLH-11, HLH-30, K10B3.5, LAG-1, LIN-13, LIN-15B, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NFYC-1, NHR-49, NHR-85, RCOR-1, SKN-1, SMA-2, SNAI-1, TBX-2, TLP-1, UNC-120, UNC-62

Targeted by: ELT-2, HLH-1, LSY-2, NFYA-1, TBX-2, UNC-120, UNC-55, UNC-62, BLMP-1, RNT-1