WormBase ID: WBGene00017326
WormBase description: Is an ortholog of human DMRTA2 (DMRT like family A2). Is predicted to have DNA-binding transcription factor activity; metal ion binding activity; and sequence-specific DNA binding activity. Is expressed in AVG; ventral nerve cord; and in male.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: GGTAGCACAGATACTGACGA
sgRNA2: TTCTTCGAATTCGTGCAGAG
5'HR: GCATGCCGCGAATCTAGTAAGATGTAAACTTTATTGAAAACACTTTCAATTTTATTCTAAGAATTTCTTGTTGTGGTGGTTGGCAGGTGATTGATGGCTCGTGATTTGGCATATGGTGATGATATTGATTCCATAACTTCCATTCAAATCTTGAGAGAAGTTGCGCTACTTGCAGCAGGATAGGAATGTGTTCACATTTTTTGAACAAAATTAAAGTTTCAATGTTTTGTGGGTACAGTTTTTGAAAATGCATATCTCCAAGCGTCACCATTTTTCAACTCCGGAATTTTGAAATATGGCAACCATGGCCTAAAACAAAATGCAGGTCAAGTGGCTTATTTGATTTGATAGTCCCTACTCACTATCAGAAACTCGCCACACCTTCTCAAGAAAGGCTAGAAGCTTTTAACCAAGTTTAGCCAACTTTCGGTAGTTTTTCAGTGCTCATTTTTGGGATCATCCAAAATTTAACCAGCTTTACCTAACTTTTGCAACTTTTGCGATAACTTGCTAAAGTTGGTTGAAACGTTGCGACATACTGTCGAGTTATAGGCGATTAAGCAAAGAGCAGTTTATCCAGTTTTTTTCGATTTAAAACTATTTGATTACCGTATATTTTCAACATTCCTAAATATAGTGCCTTGTTATACACCCACTTGATTATTACCAAATTACTCAATGTTTTCTCACAGTCTCTTATTTTTTCTTTTCCTTCTACTACTATCTCTGGATATATCTGTTTCATTTGATCCTACAGTAACCCCTCTTTCCCCGACACCCTCTTCACAAAGGGTGGAGCTTCTTCCGGTTGGTGGTCCGCGCGAGCACTGATCGAAGAGCAAAAGGGGTGGGACTTGAGCATTCCTTTTGGGAACCATTACCTCTCATTCTGATATTATAACATTTCACCTTATTCACAACAAAATATTTTAAACAGAGGTACC
3'HR: CTGCAGATGCCTGTTTCTCCGTCATCGGTGTCCGTTCTACCGCCAACTAGCCTCGAACAGATTCTTCGAATCCGTGCCGAGAGAAATCAAAGGACTCCGAAATGCGCGAGATGTCGGAATCACGGAACTACTTCTGCATTGAAAGGTTCGTATTTTAATATTCCATCAAAACAATTAACCATATTCATCAGTCTCATAGAATAAAAAATAAAAACCGAGATTCAAGCTGTCAAATTAAGCAAGACTACCAGAAAAATCATTTTGTTTAATCCTTAATCGAAGTACAAAATTTTTTTCAAGTGCATGTAATAGTTCCAGTAAAGCTTACTTCAAAATATAAGGGGACAATAACACTCGATGCTCCGGCTTCTGTTAAAACCATAAAATAAGTTGCTCTATAAGAACTTTCAATTTTTGGAAACACATCACCGATCTTGCCATCTTTGCGTGATTTGTGCTCTCGTGGAAAATCTAGAATTTAAATGTAATATTCCATAAACTTTCCAGGTCACAAACGTTACTGTCAATGGAAGGATTGTATGTGTGCAAAGTGCACATTAATTGCCGAAAGACAACGAGTTATGGCTGCTCAGGTTGCTCTTCGTCGTCAGCAATCTCAGGAAGAAAGAGACGCAAGAGATTTGGAAGTACTTCTGGGTTCTGCTGGAAATGGTAAGTCAGCTTATTTAAATATTCGGGGATTAAGCTGTGGAATGCTGGTGCTGAATCAGTGCATGTGTTTTTCCCGATCAAGTATTTCCGGTCATTTTTCATTCAAGTTGTGTCTTGAACAAGCTTCCCGCTGTGCTCGAGGTGCTAGACTAACTATTGGGTAACCTGACACTAATATGACCTATACTTGTTACAATACTAATAGTTCATTAGGGTCATCTTTGTATGCTCACACACCTTCTACAATGACACATTGTTCTCTTTTTTTATAGAATAACACGCTAACTTTTTATTTAATTTCTATTTTTTAGACTAGT
KI verification primers: DMD-5-NKIF: 5'-ACCCTTCCTTAGCCTCTTTAATACG-3' DMD-5-NKIR: 5'-GTCGTCGGAGAAGCGTGGTT-3' Download
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Strain name: SYS429 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; dmd-5 (devKi104 [mNeonGreen::dmd-5]) II
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Expression pattern: Lineage-specific, Pharynx-specific, Anterior-biased
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-2, PAX-1, PHA-2, Y48G1C.6, CEH-34, LIN-11, EYG-1, CEH-45, CEH-16, AST-1, MLS-2, CEH-27, PHA-4, LIM-7, M03D4.4, TBX-2, CEH-36, EGL-44, CES-2, Y75B8A.6
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-2, CEH-34, LIN-11, EYG-1, MLS-2, M03D4.4, TBX-2, CEH-36, BED-3, CEH-13, CEH-14, CEH-18, CEH-24, CEH-31, CEH-43, CEH-48, CEH-6, CEH-83, CHE-1, CND-1, COG-1, DVE-1, ELT-2, FAX-1, FKH-6, HAM-2, HLH-1, HLH-30, HMG-4, HND-1, LIN-32, NFYA-1, NHR-232, POP-1, PQM-1, REF-2, SEM-2, SOX-4, SPTF-1, SYD-9, TTX-3, UNC-120, UNC-42, ZIP-8, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, ELT-3, FKH-8, LIN-13, LSY-2, MDL-1, MEP-1, RNT-1, SKN-1, SWSN-7, UNC-130 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-2, CEH-34, LIN-11, EYG-1, MLS-2, M03D4.4, TBX-2, CEH-36, BED-3, CEH-13, CEH-14, CEH-18, CEH-24, CEH-31, CEH-43, CEH-48, CEH-6, CEH-83, CHE-1, CND-1, COG-1, DVE-1, ELT-2, FAX-1, FKH-6, HAM-2, HLH-1, HLH-30, HMG-4, HND-1, LIN-32, NFYA-1, NHR-232, POP-1, PQM-1, REF-2, SEM-2, SOX-4, SPTF-1, SYD-9, TTX-3, UNC-120, UNC-42, ZIP-8, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, ELT-3, FKH-8, HLH-4, LIN-13, LSY-2, MDL-1, MEP-1, PAX-3, RNT-1, SKN-1, SWSN-7, UNC-130
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