HLH-4

WormBase ID: WBGene00001951

WormBase description: Is an ortholog of human ASCL1 (achaete-scute family bHLH transcription factor 1); ASCL3 (achaete-scute family bHLH transcription factor 3); and ASCL5 (achaete-scute family bHLH transcription factor 5). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Is predicted to contribute to DNA-binding transcription factor activity. Is involved in positive regulation of transcription by RNA polymerase II. Localizes to RNA polymerase II transcription factor complex. Is expressed in ABalppppaav; ABpraaapaav; ADLL; and ADLR. Human ortholog(s) of this gene are implicated in congenital central hypoventilation syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACATTCGCATCGTCATTAGC-3'

Strain name: SYS110

Cross: OP523 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs523 (hlh-4::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): DAF-19, LIN-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: FOS-1, NHR-6, PAX-3, SKN-1

Targeted by: FKH-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: DMD-5, HLH-30, NHR-6, SKN-1

Targeted by: FKH-8