Single-Cell Protein Expression Atlas of Transcription Factors

DAF-19

WormBase description: Is an ortholog of human RFX1 (regulatory factor X1); RFX2 (regulatory factor X2); and RFX3 (regulatory factor X3). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in positive regulation of transcription by RNA polymerase II and regulation of non-motile cilium assembly. Localizes to nucleus. Is expressed in several structures, including body wall musculature; excretory cell; intestine; neurons; and somatic nervous system.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: GACCTTGACCTCGAGAAAGC

sgRNA2: GTGAGATCTATGGTAGAAAA

5'HR: GCATGCAACTTTGGAGTTGATCGAGATCACATTAGTGCTCTTTGTACACTCGATCAAGTTCAAGACTACATTATTGAAGTGGACCTTGCATTGTACCAAACCATCGTCGATACTCTTATCCCAAACGTTTTGCTCTCGGAGCTCTCCACAGGAATGACCCAGACGTGCCGAACATTTGCTAAAAATATTGATGTGTACTTGAGGAAATCGCTGATGCTCGCAAATCTCGGAGAGTTTTTCGTAAAAAAGAAGATCCAGGCAATCAAGTATTTGCAACAAGGGCTCAAACGATACACTTCTTTGAATCATTTGGCTCACGCTTCTCGTGGAGTTTTGATGAAACCAGAGCAAGTTCAGCAGATGTATCAAGACTATATTCGAGTTGATATCAACACAGTCCACCAGCAAGCCGGATGGATTTGTGGTTGCGATAGTGTCATGGTGCACCATGTGAACAACGCCTTCAAGCATAACCTTCAACGGATGAGTGCAATGGAGGTATGGGCCGAGTGGCTGGAAAGTATTGTGGATCAAGTGCTCGCCAAGTATCACGACAAACCGGCAAACGTGATTGCTAATGTCGGAAAGCAATTTCTGCTCAACTGGAGTTTCTACACATCGATGATCATTCGGGATCTGACTTTGCGATCAGCCATGTCATTCGGATCTTTCACTCTGATTAGGTTGCTGGCCGATGATTACATGTATTATTTAATTGAGTCGAAAATTGCTAAAGTGAGTTTATTCACAAGATAAAGAATTATCTATAAGAACATCTATTTCAGGCCGGAAAACAACAACTCATCACGGTAATTCGTGCCGACAAGGACTGGCCGTTGACTACAAACCCTCAGGAATACATAGTGGTAGCCAATGACAATGACGAAGACCTTGATCTTGAAAAGGCAGGTCTTCTGGGTACC

3'HR: CTGCAGAAAGTTCTCAATTTCTTTCACCTCGATTTTCTACCATAGATCTCACTTCTCCTAAAATGTTCTAAAATTCTAGTCTCCCGTCTCCATCTTGCCCCTCTCCCGGATAAAAACTGTGACATAATTAGCACTGTAGTTCAATTCATTTTGTTTTCACAAATTGCTTTCCACGAAAGCTTCTACCCCTATGATCTCTTCTACACAAACCCTAGTGATATACAATGCCAATTTCCAGTCAGATTATCGTTTTCAGAAAAAATTATAATTAAAAATTCTATAATAATGCCAAGAGATATTTCCCCTTATTTATCGAAATTTGGCCCCTATTTTCATGTTTTGTGATTTGCATTTCTCTCCTCTCAACCTACTTCATTCGAAGAAATGTTTTGGTTTTCATTTTCATTTTCGCCTTCATAAAGGTTTTTGTGGTACAAATTGACTATTTTTGGTAGAAAATATGCTTAAAAAATTAGGTTAAAGTGAGTTCTTGGGAAGAGAACAAGAAAATAGATGTCTACTGACTATGAGTGGGTGGATGTTGGCAGGAAGAAATACTGCAACAGAAATGAGCTGAAACTTAGAAGAGCAAATGAGTGGCTCAAAATTGAACGACCCAGAAGGAAAGTACAAACTAAAAAACCCTCTCCCATTGTGTTGAGTTATGCAAACTTGTTTGAAAACCTAATATTTTTTCAGTTGCCATTTTTCTATTTATAGTTCCAGTGTTCAAATAATTTCGAATGAAACATAGCATAGCCTACTAATTATTTATGTTTTTGAATTTTTCAGATATTTACTGTCTGGAGCTCTAAGAAGATGTGTTTTATGTGTACCCTGACTCACAAAAATAATATTTTGCTATGAAAATGTAAAAGTTTTGGTACAAAATGTAAATTTGTGATGTGTTTGTTTGTTTTTGAAATTTTTATTCAAAATTAGATTTTGCGCACTAGATAATACAATTTTGGAATTGAACTGGACTAGT

KI verification primers: DAF-19-CKIF: 5'-GCATCAGTAGAAGACACTTGGAGC-3' DAF-19-CKIR: 5'-TGGTCAATTGTATTTTAAAGGGTAGAC-3'

Download

Strain name: SYS541

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; daf-19 (devKi164 [daf-19::mNeonGreen]) II

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): HLH-4, LIM-4, LIN-11, EGL-46, CEH-48, NHR-67, HLH-3, TBX-2, EGL-44, FKH-2, CEH-43, CRH-2, ELT-6

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-48, TBX-2, CEH-43, ALY-1, BCL-11, BED-3, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CEH-39, CEH-83, CHE-1, CND-1, COG-1, DMD-4, DVE-1, ELT-1, ELT-2, ETS-4, EYG-1, FAX-1, FKH-6, GMEB-2, HAM-2, HLH-1, HLH-8, HMBX-1, HMG-4, HND-1, K09A11.1, LIN-32, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-2, PHA-4, POP-1, PROS-1, REF-2, RNT-1, SEM-2, SNAI-1, SOX-4, SPTF-1, SYD-9, TBX-8, UNC-120, UNC-39, UNC-42, UNC-86, ZTF-11, BLMP-1, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, FKH-8, FOS-1, IRX-1, PAX-3, SKN-1, SWSN-7

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data