LIM-4

WormBase ID: WBGene00002987

WormBase description: Is an ortholog of human LHX6 (LIM homeobox 6) and LHX8 (LIM homeobox 8). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in cell fate specification and regulation of transcription, DNA-templated. Localizes to nucleus. Is expressed in several structures, including ABalpppppa; ABpraaappa; head muscle; head neurons; and somatic nervous system. Human ortholog(s) of this gene are implicated in several diseases, including congenital ptosis; myopathy (multiple); and nail-patella syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ATTCAGGCGACTGATTGGGTAC-3'

Strain name: SYS225

Cross: OP681 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs681 (lim-4::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): DAF-19, UNC-42, CEH-48, NGN-1, HLH-3, SPTF-1, FKH-2, EGL-44


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-42, CND-1, FKH-10, NFYA-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-42, NFYA-1, FKH-10