WormBase ID: WBGene00001981
WormBase description: Is an ortholog of several human genes including FERD3L (Fer3 like bHLH transcription factor); MSC (musculin); and TCF24 (transcription factor 24). Is predicted to have protein dimerization activity. Is expressed in several structures, including Daa; Dap; Dpa; Dpp; and somatic gonad precursor. Human ortholog(s) of this gene are implicated in several diseases, including Barber-Say syndrome; Sweeney-Cox syndrome; and synostosis (multiple).
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: TCGCTAGCTCATCTACAAAA
sgRNA2: TCTTGACTTCTTGGAATCAG
HND-1-NKIsg3: ATATCACTGACTTGAATCGG
5'HR: GCATGCGATGTCAGAAAACAATGCTTGCTTTTATCAAATATAAATGTAGATAAGCATTTTTTGAAATAATGGAACACAATCAACAAATATTCACAACTAAATCCCTTAATTTCTGTGCATCAATAATGCCAATGTCTCTACCGTATGATATCAATTGGCTCACTCTGTAATCTAAACAAAGTGTAATTGAGTCCGGCACGGGTTTGTCGGCATCTTTTCGAAGAGGGGTGATTGCGCGAAAGCCGTGCGTCGGGATGCCCAGACAGGGCGGCAAGCGGCTAGTTTTTGATGGCGGGGGATGGTCCGGATGGGAACTCGTATACCGAGAATTCATAAACATTTGTAGACTGTATCTGAACTGTGATTTACTGTGTTGATACTAGTGGATTCTTTAGAAAAGTATCTTAAACTCAAAAAAAAACTTTCTTATAGTCATATTAACTTATGTAGCAATAGTATTTGAATAAATTTTATGACGTCAGATAATAAGTTTCAATAAACATCGCAGCACTAATTGTAAGTGAATAATCCTGTTACTTTATTTGAGAATATAAAAATTATACTTATATTTTTGCCTCAAACTCTGATGCCATGCATATTTCACGGATAACCCATATTAAAAAGTCAAATATCGGAAGTCTATTTTTTTGAACATTTTTGGATAACCATGTACATATACACATCATCAATTTTACAACCCCTTGCCATTCCAGTTTTACAGCCTGTCTTTATACAAAAAATCTCCACCTCAACATAAGTATAATGTCAACAACAAAAACATATTCCAGGCCGGATAGTAGTCTACACAGATCCTCCCACCATCGTGAACCTTTCCTGCCCGCATCTATCCTCCCTTGCGGGGTCTGTCTTCTTGCTCCGCCCACCCGTACCCATCTTGCGTACCTCATTCCTCCGATTCAAGTCAGTGATATATCGCTAGCTCATCTACAAAGGTACC
3'HR: CTGCAGATGGTCAAATCAACAACTGCTGGAAACAATGCGGTTTCTTCATTAGAATCTACTGACTCCAAGAAGTCAAGAAAGGAAAAGTCTCGCGAGAAGGAGCATCGTCGTGCTCAATGTATCAACTCGGCCTTTGAGATTCTTCAGCAGTAAGTTGTTAACATTTCTTTTCCCTAAACAAATAGCTAACTTAATCTTATTTTCAGACATATTCCATACCTCAAATCGGAAGAGAGAAAGTCGCTGCCAAAGATCAAAACGCTTCGTCTTGCCATGCAGTACATTGATCATTTGAAAAAATTGCTGGGAGGAAATGAGATGGTATGTCGAATCATAATGTTTGTAACTGTAGTAATACTTTTTCGTACCATCAGTCAAAATGATCGTGTTTTGAATTATCTACTGTTTCAAATATCAAATTTCTTCAAATTCCTAACTCTTGTACGTTTTTTCAGCTCGAAACCGATTGCAACGAATCTCGCCCACTCACTCACTCAGATTTCCGTGTCAATATCTCGAATGAGATTAGAATTCGGAACAGTTACCGAGAGAGAGCCCACAATCAGGAGTTGGATGAAGCAACTGTGAAGAGAATTTTGGCTCGCGAAGATCAACGCCGTCGCTGTAGCTCAGTTCCGGAGGGAATACAAAGTAAGATCATAAAAATAGAGACATGGAAAAACAATTGAAATTTTCAGGATATACTCCTTACCAGAGCCGTCAATTCGGACCGATTTCGAACAATGTGTGCAACTTTCGCCACGTAGATGCTAACCAATACAACACCAACTTCATGGAATATCAAACACAAATGGTCCAGGTCGCCAACTTCCCAAATCAGTTCTCGCATGAGTATTACAACTATGGCACATTCCCAGTTATTGATCCAACTTCATTGGAGAACAGTAATCCAATTTTGCAATAACATTGTCTCATGTTATGTTACTCACGGTTTCCTTAATTGATCTCTATATTTCGACACTAGT
KI verification primers: HND-1-NKIF: 5'-TATAAGGCGTTTTCTCTACTTAGCAAG-3' HND-1-NKIR: 5'-TAGGATTAGAGGCCACCAGGG-3' Download
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Strain name: SYS676 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; hnd-1 (devKi213 [mNeonGreen::hnd-1]) X
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Expression pattern: Lineage-specific, Muscle-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): UNC-30, FKH-4, LSL-1, LET-381, REF-1, PAL-1, VAB-7, UNC-120, HLH-1, M03D4.4, TBX-9, HMBX-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: CEH-2, DAF-19, DMD-5, ETS-5, K09A11.1, LIM-7, NHR-102
Targeted by: NA |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: CEH-2, DAF-19, DMD-5, ETS-5
Targeted by: NA
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