CEH-39

WormBase ID: WBGene00000460

WormBase description: Is an ortholog of human ONECUT1 (one cut homeobox 1); ONECUT2 (one cut homeobox 2); and ONECUT3. Is predicted to have DNA binding activity. Is involved in negative regulation of transcription by RNA polymerase II and primary sex determination. Localizes to condensed nuclear chromosome and germ cell nucleus. Is expressed in body wall musculature; germ line; gonad; neurons; and ventral nerve cord.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACAGGCAGATTGGCGATGAT-3'

Strain name: SYS401

Cross: OP169 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs169 (ceh-39::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): TBX-37, TBX-38, MED-2, LSL-1, T07F8.4, REF-1, TBX-11, CEH-83, HMBX-1, HLH-3, TBX-9, ELT-1, SEA-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, CEH-20, CEH-38, CEH-40, CEH-44, DAF-19, DAF-3, DPFF-1, DUXL-1, EFL-1, EGRH-1, EOR-1, ETS-5, HLH-2, LIM-7, LIN-13, LSY-2, MEP-1, NFI-1, NFYA-1, NHR-102, NHR-49, POP-1, RCOR-1, SPR-4, UNC-37, UNC-62, ZTF-6

Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CND-1, COG-1, ELT-2, FKH-10, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LIN-32, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PQM-1, REF-2, SPTF-1, TBX-2, TBX-8, UNC-120, UNC-42, UNC-55, UNC-62

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, CEH-20, CEH-38, CEH-40, DAF-19, DAF-3, DPFF-1, DUXL-1, EFL-1, EOR-1, ETS-5, HLH-2, K10B3.5, LIN-13, LSY-2, MEP-1, NFI-1, NFYA-1, NHR-49, POP-1, RCOR-1, SPR-4, UNC-37

Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CND-1, COG-1, ELT-2, FKH-10, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LIN-32, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PQM-1, REF-2, SPTF-1, TBX-2, TBX-8, UNC-120, UNC-42, UNC-55, UNC-62