Single-Cell Protein Expression Atlas of Transcription Factors

NHR-49

WormBase description: Is an ortholog of human HNF4A (hepatocyte nuclear factor 4 alpha) and HNF4G (hepatocyte nuclear factor 4 gamma). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in determination of adult lifespan; positive regulation of transcription from RNA polymerase II promoter in response to stress; and regulation of fatty acid metabolic process. Localizes to cytoplasm and nucleus. Is expressed in hypodermis; intestinal cell; muscle cell; and neurons. Is used to study alcohol use disorder and obesity. Human ortholog(s) of this gene are implicated in maturity-onset diabetes of the young type 1 and type 2 diabetes mellitus.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: TGGAGAGGATGAATTGCCAA

sgRNA2: ATTCTGCTCACTGTTCAAAA NHR-49-CKIsg3: ATGGAATCTTGTGATTGGAG NHR-49-CKIsg4: GTTGCTGAGAGCATTCCAGT

5'HR: GCATGCTCTACTGAAAAACTATGCACCACAACATCTCATTTTGATGCCAGCATTTCGAAGTCCCGATACAACTCGAGTTTGTCTCTTCAACAATACTTATATGACACGTGACAATAACACTGACCTCAATGGATTTGCTGCATTCAAAACAAGCAATATAACACCCAGAGTTCTTGATGAAATTGTCTGGCCAATGCGACAACTTCAGATGAGAGAACAAGAATTTGTATGTTTGAAAGCACTTGCATTCTTGCACCCAGAAGCCAAAGGACTCTCGAATAGCTCACAGATTATGATTCGTGATGCTAGAAATCGTGTTTTGAAAGCTCTTTATGCATTCATTCTCGATCAGATGCCAGATGACGCACCCACAAGATATGGAAATATTTTGTTGTTGGCACCGGCTCTCAAGGCTCTGACTCAGCTGCTCATTGAGAATATGACACTTACAAAGTTCTTCGGATTGGCAGAGGTATGAAAGCTTTTCTTGTTTGAATCCGTACATAAATGATAGCTTCAGGTGGATTCTCTGCTCTCCGAGTTCATTCTCGACGACATCAATGATCATTCGACGGCTCCGGTCTCTTTACAGCAACATCTTTCATCTCCGACAACGTTACCGACTAATGGGGTTTGTTTTTAATTATTTTATTAAACTTTTTATTCTAAAAAAATACAAAAATAGCGAAAACGGCCAATGATTTCAGGGAAAAACCGTAAAATTCTCTTTTTAAAAAAGATCTTCCATTTCAAAATTCATATTTTTCAGGTATCTCCGTTAAATCCAGCCGGATCAGTGGGAAGTGTTTCGTCTGTTTCTGGAATTACACCAACTGGAATGCTTTCGGCCACTCTTGCAGCTCCATTAGCCATCCACCCTCTTCAGTCACAAGATTCTATTTTAAACAGTGAGCAGAATAATCATATGCTCGGTACC

3'HR: CTGCAGTAATTCCATTTTTTCTCCCAAAACTCTTCACCTCATCTCATCTCCAGTTCTATTCCGAATCTGTGATCTTTCATCTTTTCCCTTATTGTTGTTTTGAATCATTACCCCCAATTTATAAAATAGTCCAGAGCCCAGCTATAATGTACATAACAAAAGAAGAATACCCTTCCGTCTCAGTAGTTGTTTCATCTTGAAACCTGTCTAGGCCATCTCTATATCATATCAGGTTATTGTTCTCGTCTCCCCCAACTCTTCTTCCTTAATCTTTTTTCCAGACGGGCTGGCTCTCGTTTATTCTTGTGATTATTATTGTCAATCAAATTATCAGTTTAGTACTTCAATATGTATAAGAAGTGACTGTCTTGGCATTTCCCATCTCTATTTAGAAGATTCCCTTCTTCCCATTTTCCTTCCTTCTTTTCAAGCGTTCGTACCAATTTATGTTTGCAAAAAGAAACTTTTATTTATACTTTTTCAAAGCTCATCGATAAAATACATTATTATACCATATTTTATTATTCGTATCAAATAAACACAATTGACTCATCATTATGAATTCACTCGAGAAGTTCAATGCCACGTCCTCCACCACCTCCACCAAGACCGTAAGTTTTATTGCAATTTAGAAATTTAGAAGAATTTACAAAAAGTATTGTTTTCCAAATGTTTGAGCACCTGGTGTGAGCTGCTGGAGGTGAAGCTGAGCTTTTTAGTTTTTTTTTTCAAAATTAGATTTTTTTAAATTGTTTATTTCTCTAAAACTCTTCAAATTGATGATTTTTGTTTTGATTTCAATTGACACGATCATCTAAATGATAGTGGATATATGACCATTTCCCAAGACACTTGAGATCAATTCAATGATCCGAACAGTGTACATAACGGGGTAAAAGTGATGATATGTCTATTGGAAGAGGGAAGATGAAGAAATTGTGAGCATTTAGGCTTAGGTACTAGT

KI verification primers: NHR-49-CKIF: 5'-TATTGATGGTTGAATGGGCAAA-3' NHR-49-CKIR: 5'-AATGTCCAAAACTGGCTGAAGTG-3'

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Strain name: SYS696

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; nhr-49 (devKi169 [nhr-49::mNeonGreen]) I

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: BCL-11, CEH-14, CEH-18, CEH-31, CEH-34, CEH-39, CEH-48, CEH-83, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, HAM-2, HLH-1, HLH-30, HMG-4, LSY-2, M03D4.4, MEP-1, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, ZIP-8, ZTF-11, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, FKH-10, FKH-8, FOS-1, IRX-1, LIN-13, MDL-1, MEF-2, NHR-85, PAX-3, SKN-1, SWSN-7, UNC-130, UNC-86, ZAG-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data