WormBase ID: WBGene00006773
WormBase description: Is an ortholog of human TLE3 (TLE family member 3, transcriptional corepressor) and TLE4 (TLE family member 4, transcriptional corepressor). Exhibits transcription factor binding activity. Is involved in several processes, including chemical synaptic transmission; regulation of axonogenesis; and regulation of synapse structure or activity. Localizes to nucleus. Is expressed in several structures, including germ line; head; and tail.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: AAATTAAGCTTACTCGTTGC
sgRNA2: AAAAATGAAGGCATCGTATC
UNC-37-NKIsg3: GCTCGTCTTTGATTCGATCG
5'HR: GCATGCGATTTCCTAATGAGTTTAAACTCTCGAGTTTTTATATGTGTAATATATTCTTCAACACTCCGGAATGCTTCTAATTAGTTACAAATGACAAAATGGAGCTTCATTCATGTTTTGGTTTTTTTTTAAATTTTCATGACTGCTACAGTATACCGTCTTGTTGTTTAATCTAATAGAATCATTCAAGAGTTTTTATTCTTTCCATTTGAATCTCAATTTTCACTATTATCATTTGATTCTCATCTCATTTTCTTACCGATTTTCAATCTCAAATTATTTAAGAACATACCATGTCAATAAACTTATTATTTGTTAAACATGATTTTTTAAAAATATTTTCTTCAAGACAGGTTTATGTCTCTTTATTCAATATTTCAACCTTTTCCGTTTTTTTAGATAATTTTGTGAGTTTTTTATACTGGAATTGTGATTATGTACATGTAAAATATTTATAAATGCTTCTCTCTATAGTAAGTTGCAGCTTCATCGTGTTTGATTCGAAATATTAGTTAGAAATCCTCGGATCCTCGTGTTACTTGAATATCAAACTTATTGGATACATTCATCTTCTACACTCGGGCTCAAAAGAGCTCAAAACAAATGTGCTCTATCGACAACTCCTCCCCCCATTCGGTCACCCATTCAGCGTCGCATTCATCGCCGCGCGTTCGTCCTCCTCGACACCAATACCTCCTTCATTCTTCAACGTTAGTGGGGCTATATGACCAGTCGTTTTGCAATTAATGTATGATTCTCTCCGTCTACACCACTGGAAACCCATGTCCTCTTTCTTTGTTTCTTCGTCTCGTTGTCAACGTCTATTCAAACCACGTGCTATTTCTTGAGTTTTGTTGTCTTCTCCCAATCCATCACTAGTCTGTTCTTCCTTGCATTTTTTTGATCGAATTTTTAAAACTCCTCTCATAATTTTTATGTTAATAATTCACTATCCACTTGATTGTATTTAATTTTCAGTAAAAGGTACC
3'HR: CTGCAGATGAAGGCATCGTATCTTGAAACTCTCGATCGAATCAAAGACGAGCACGCGGAAATGTCGAAGCATGTCAATCAGCAGCGAGTAAGCTTAATTTTGTAAGAGAAATTTTAGTATATTGAAAACAATTTTGCAGTCGGATATCGAAAAGGTTGCGTTAGAGAAAGAAAATATGAATAGATCGTATATGACGTACGCTGAAGTATCAAATACTCTTCGTAGCGATCTTCGAAAAGCAGAAGAAATCAACAAACGCCTTCAGGAATTCATCATGCAATCGGTAAATTAACTCGTCAATAATGTTATTAAAAAATCGAACAAAAATATAGAAAAAGCTATTACAATTTTTTTGTTGAAATATTTGAACTAAATATTATGGAAACGTTATAATATCTATTGGATTGTACCTTGTTCAACTCAAACATGCTCCTCTTTCCATATATTCATTTATAAATAGAAGAAAAATTATTATGTAATATTTTTAATTCATAATAATCAATTTTAGGTAATATTATAAATGTATATTTTTCAGTTGGCTCCTCAACTATCTCAAGATAATCAAGCAAACTGCCTCGCCGCACTGGAAGCTTTCAAAACCGCATCACCACGTGAAAATGGAAATGGAGCACCAGCACTTCCACCAGGTTTTCCTCCCGGTGCAGCTGGAATGCTTGGAATGATGCCAAATATGCCATTCGGAATGAGCCCTGCAATGAGTCAACTATTCAATCAATTCGCATCACCACATGTCAACGGTGGAGATGGAGCCGGTGGAAGTTCTGGAGGTGCAAGTGAAGCGAAAAAGGCCAAGCTGGAAGATCCAGATGATGGAGAACTTGAGATTGATGTCACAAATGATGATCATCCAAGTACTGCATCAAATGGAGGCGCAGCTAATAAAAATGGAAGAGATAGCACAAATAGTGTTGCCTCATCGGGAGCATCTACTAGT
KI verification primers: UNC-37-NKIF: 5'-GATCAGTTAGCAATGAAACGACAAT-3' UNC-37-NKIR: 5'-CTTAGCACACTTCAAAAGATTGTAAAC-3' UNC-37-NKIF2: 5'-TGAAATGATTGATCAAAGGGCATA-3' UNC-37-NKIR2: 5'-GAATCCTCCAGCAGCACTAGCT-3' Download
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Strain name: SYS681 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; unc-37 (devKi218 [mNeonGreen::unc-37]) I
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Expression pattern: Ubiquitous
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-39, CND-1, COG-1, DVE-1, ELT-2, FAX-1, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, BLMP-1, CEH-82, DPL-1, DSC-1, EFL-1, FKH-8, FOS-1, MDL-1, SKN-1, SWSN-7, UNC-86, VAB-15, ZTF-11 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-39, CND-1, COG-1, DVE-1, ELT-1, ELT-2, FAX-1, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, BLMP-1, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, FKH-8, FOS-1, MDL-1, SKN-1, SWSN-7, UNC-86, VAB-15, ZTF-11
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