WormBase ID: WBGene00012317
WormBase description: Is an ortholog of several human genes including AC008481.3; ZBTB32 (zinc finger and BTB domain containing 32); and ZFP91-CNTF (ZFP91-CNTF readthrough (NMD candidate)). Is predicted to have nucleic acid binding activity. Is expressed in several structures, including head muscle; hyp7; hypodermis; postdeirid sensillum; and tail. Human ortholog(s) of this gene are implicated in autosomal dominant non-syndromic intellectual disability 22.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: GACGTCGGAACGTGTTAGCG
sgRNA2: TGTCTGAAACGTGTGATGGT
5'HR: GCATGCTTCAATTCAAAATTGATTTACCTATCCTGACATATTGTGCTGTTTTAGGAAAAAAAATTAGCTTACCTCCCAGTTTTCGACCTTTATTTTCCACATTTCCCATTACAGATTGCTGTTTTCATCTTGAAATTTTTGGGTTTTGACTAGAAAATTGATTTTTCCAGCTTAATTCTGGGTTCTTCTCTACATTTTCACTTAATTTCCACTATTTTCCACAGTTTTCGCACTGTTTTTCAATTTTTTCTCCGCTTGAAATTCTCGAAATTTGAATAGAAATTTGATTTTTTGCGGCTAAATTCTTCATTTTTTCTCACTACATTTTTCCCGTAATTTCCCAATTTTATTTTGCATTTTTCACGTGGTTTCAGGCTGTCCCTTTCGGTTTGATCTACGTTGATCTACAAGAAATTGCATGGTTAAGAACGTGCTAATGTCACATTTTTTGAGCAAAAAAAAACCCGCATTTTTTGTAGATCAAACCGTAATGGGACGGCCTGGCAACACGTGATTTTCCAAATTTTCTGCTCCCGATTTTCCAGTAATTTCCCTTCCAAAAATCGAATTTTTCCATTAATTTTGAGCTATTTCTATAATTTTTAGGCTAGAAATTTGTTTCGAAACGCATTTTTTCACTAAAAAATTTCTCTAAAATTTGTAATTTTTTTTCGAATTTCCGCAGAAATTACACTTTCTCCGAAATTTTTATGTTGGAATTTTGCTCCCAAAAACTCAATTTTTGAAAAATTCAAATTTTCAATACTTCCCTGCCATTTCTCTAATTCCGTGCATTTTTCCACCTTTTTCCACCGATTTTTTTTCTAAAAACCTCCCTTAAAATCAAAAATCCGGCTAATTTTTCCCAATTTTCAGCCAAAAATTAGACTAGAATACCATCAACCACCACCTACACCACCATCACCATCATCAAGAACAAGGTACC
3'HR: CTGCAGATGGGACCGGATACGACGTCGGAACGTGTCAGCGAAGATCTTATGCAGGCTGTCACATGTCTCAAGCGCGTTATGGTCGGTATGAAGAGCAATGGGACAGATTATGTTCAGGAACAGGTGCGGCTACCCGAAAATTTGAAGAAAAACGATCAAAAATTGACGTTTTTAAGACAGTTTTTCGGTTTTCTCATAAAATTTTAGTCCAAATCACTGAAAAAATGGAGAAAAAAGGATGAAAATGGACTACAAACTACAACTTTTATTAGCCTCAACCATACAAAAGTTGGGCTAACGAAGCTTTGTAGTTTGTAGTCACTCGCTCCTCCATTTTTTTGGTTTTTTTTTTAATTAAAAAAATTCTTCTAACTAAAAAATCACCGCTTTTTCTAAAATTTCAGCCATTTACCAATTTTTCAATTTTCAGACTCCCTCCAACAGCACTACCGTAATATGCACACTTGTCAAAATCACCGGAGCACTGGAACGATGCATCGAGAGCAATAAAGCGATGAATGATGTGCTCAACGGGAAAATGGTGAGCATTTGAAAAAAATTGTGCAAGAATGCTCAAAATTGTGTGTGTGTAGTTTGTAGTCTGAACATTATAGTTTGTAGTTTAGCATCCGGTGACTGCAAACTACAAATTTAGCTTGAAAATATAATTTGAAAAGTTGAAGCTAAAAATTGAATTAAATCGCAAAACTTTAACGAAATTTTCAACAACTTGATTTCGTAGTTTGTAGTCTAAGTCCTTTGTAGTTTGTAGTATGGAGTATAGATACTACAAACTACATTAAATGGCTCTGGCTTCAAAATGTAAAAAATCGAAATTTCTAGGATATTTATTCCTCAACATAGACACTATAAACTACAAAGTTTACAAACAAAGAGGCTGAACAAGTTTTGTAGTTTGTAGTCTAGACCTCAACACAAGAACTACAAACTACACAGATCTTTGAGATTCTGCTAAAGTTTGACTAGT
KI verification primers: ZTF-6-NKIF: 5'-GTAGAGGTCCAAACATACCTATTTTGA-3' ZTF-6-NKIR: 5'-GACGGAGGGGAGGGATAATTC-3' Download
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Strain name: SYS464 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ztf-6 (devKi122 [mNeonGreen::ztf-6]) I
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ALY-1, BCL-11, CEH-13, CEH-14, CEH-18, CEH-19, CEH-2, CEH-22, CEH-31, CEH-34, CEH-36, CEH-39, CEH-43, CEH-6, CEH-83, CHE-1, CND-1, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, ELT-4, EYG-1, HAM-2, HLH-1, HLH-30, HLH-8, HMG-4, LIN-32, M03D4.4, MEP-1, MLS-2, NFYA-1, NHR-12, NHR-232, PAX-1, PHA-2, PHA-4, PROS-1, REF-2, RNT-1, SOX-4, SPTF-1, SYD-9, TBX-2, TBX-7, TBX-8, UNC-120, UNC-42, UNC-55, UNC-62, ZIP-8, ZTF-11, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, FKH-4, FKH-8, FOS-1, IRX-1, LIN-13, LSY-2, NHR-85, SKN-1, SWSN-7, UNC-86 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CEH-36, CND-1, LIN-32, NHR-12, UNC-42, ELT-3, ZTF-11
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