WormBase ID: WBGene00002245
WormBase description: Is an ortholog of human RBPJ (recombination signal binding protein for immunoglobulin kappa J region). Exhibits Notch binding activity; double-stranded DNA binding activity; and sequence-specific DNA binding activity. Is involved in several processes, including dauer exit; oviposition; and positive regulation of vulval development. Localizes to RNA polymerase II transcription factor complex. Is expressed in Z1.ppp; Z4.aaa; and ventral uterine precursor. Human ortholog(s) of this gene are implicated in Adams-Oliver syndrome.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TGATGGTGTCGTCTACTCGT
sgRNA2: TTACTAGATTCCACTCTCGC
5'HR: GCATGCTTCCAAATGATTGACAATGAGCTCGTCTATCTTTGTCTCAGTCATGACAAGATTATTCAACATCAAGCCACAGCTATCAATGAGCATCGTCATCAGATCAACGATGGAGCTGCGTGGACAATCATCTCGACTGATAAGGCTGAATATCGATTCTTCGAAGCGATGGGACAGGTTGCGAACCCGATTTCTCCATGTCCAGTTGTCGGTTCACTTGAAGTTGATGGACATGGTGAAGCGTCGCGTGTCGAGTTGCATGGAAGAGATTTTAAGCCAAATCTGAAAGTTTGGTTTGGAGCAACCCCGGTTGAAACGACGTTCAGAAGTGAAGAGAGTCTTCATTGTTCGATACCACCTGTGAGTCAGGTTCGAAATGAGCAAACTCACTGGATGTTTACGAATAGGACAACCGGCGATGTTGAGGTAATATATTGGATTTGGAACACAATGAGAATAGAGTTCTGAATTCAGAATTATCACAAAAGAAAAACGAAAAAAATCGAGCTATTTTAAAATGTGATTTTTCAGTTTTGGCTGATTTTCCTGTTTGGATTCGCTCCAGAATGAGAACTGCTAGGCCAATCTTATTTTGGAGGGAATTCAAACATCCAGTCACAATGAAAAATTTCATTTATTAATTATGTTGAAATTAACGATCGATCCGTGAATTTTGCACTGAAAAAGAGGCGAAGCATTTTTCCGCAACCCTGCGGCACAATTTTTTCCTCTTTTTCTGTCCGAAAAAGGAGAAAAAACCGTGACATAGGGTTGCGAAATTACGCCCCGCCTCTCTCTCTTTGCGACCCTGATTGGGTTAATTTCAATAGTAAAGTCGAATTTTTCATTCTTATTCGATTTTTCTTTGTTTTGTGAACTATCTGAATTCAGAACTTTTTTGTTGGTATTTCGAAGTCAAATTCCCAATTGTTTTCTAATCTCCAATTTTTCAGGTTCCAATTTCATTGGTACGAGATGATGGTGTCGTTTACTCATCAGGTCTCACATTTTCCTACAAATCATTGGAACGACATGGACCGTGCAGAATTGTGTCCAATTACGGTACC
3'HR: CTGCAGATTCCACTCTCGCGGGATTACTGTATCTTTATATTGTCTCCTAATTTCTCCCAATTCGTTTAAATTTCCACCCCGGGTCCTGAAGCCTTCATTTCTACATTTTTTTCTCGCTTCCATATGTAATAATCACTTTTAACTTACTTCATTTTCTTGTTGCAATACTTTTTGTTTATTTTCTTTTTTCCTTCTTCGGGTGGCTTTTCCATCATTTTGTTCTTGGATTTATTATTTGCAATACTTTGCCGTTTCTCTGTGTCTCCCCCTTCTACATTCATCTCTCAGCACTCTCTAATTACAGTATTTTTGAGAAAATCCTGTTGAAAATGTTTCGGATTGAATTATACTGGATTTTTCTCTGTTCTAATTATAATCAACGCGCCCTCATATTAATTAAGTATATAGATACATAGATATAATTAACATTCACACGGTGGTATCTCCCTTTTTTTTCGGATAATCTCTTCAAGTTTTTCCTTTTCTTTTGTCTCCAACATTTTCCTTACTGAACATAAAAATTTTGAAATTAAGCCTTTTTTATCAACACATGTTTATTCAGTTATTCCGATGTTCTCTCTTTTTCAGATAAATTATCAACTATCCACGCATCCGATTCGACATGATGTATCTCGGATTTTGTGGAACTTCCAGATTCTCGTCGGTGTACATTTGACGCCATTGTCCGCCATATGTTTGGCTCTGAAGAAAAATTTCTATATTTTCTAAACACTTTGTAATTTTAAATTTCATCGATTGTTTAGAAGATTGAGAATGGAAGAATTGGTATTTGAAAACTTGGGGAAACAATTAATATATTTTTGAAACAGGAATAAATTTTCCAGTAGAAACGATTTTGGATTCAAACGGTGAGAATTTTAAAAGAACTAGT
KI verification primers: LAG-1-CKIF: 5'-TTAAACTGAATTTCAGGTCGATAAGC-3' LAG-1-CKIR: 5'-TAGTGTTGAGGCTCTATGTTTTGATG-3' Download
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Strain name: SYS671 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lag-1 (devKi208 [lag-1::mNeonGreen]) IV
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Expression pattern: Lineage-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): C16A3.4, CEH-22, DMD-4, PQM-1, CEH-10, MDL-1, ELT-2, ODD-2, PAX-1, AST-1, PAG-3, TBX-7, NHR-12, BAZ-2, ELT-7, TAB-1, PHA-4
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-22, DMD-4, ELT-2, BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CND-1, COG-1, ELT-1, ETS-4, EYG-1, HLH-1, HMG-4, K09A11.1, M03D4.4, MEP-1, MLS-2, NFYA-1, PROS-1, REF-2, RNT-1, SEM-2, SPTF-1, SYD-9, TBX-2, TBX-8, UNC-120, UNC-42, UNC-62, ZIP-8, C16A3.4, PQM-1, MDL-1, ALY-2, CEH-82, DPL-1, DSC-1, EFL-1, ELT-3, FKH-10, FKH-8, IRX-1, LIN-13, LSY-2, NHR-85, SKN-1, SWSN-7, UNC-130, UNC-86, VAB-15, ZTF-11 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-22, DMD-4, ELT-2, BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CND-1, COG-1, ELT-1, ETS-4, EYG-1, HLH-1, HMG-4, K09A11.1, M03D4.4, MEP-1, MLS-2, NFYA-1, PROS-1, REF-2, RNT-1, SEM-2, SPTF-1, SYD-9, TBX-2, TBX-8, UNC-120, UNC-42, UNC-62, C16A3.4, PQM-1, MDL-1, ALY-2, CEH-82, DPL-1, DSC-1, EFL-1, ELT-3, FKH-10, FKH-8, IRX-1, LIN-13, LSY-2, NHR-85, SKN-1, SWSN-7, UNC-130, UNC-86, VAB-15, ZTF-11
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