Single-Cell Protein Expression Atlas of Transcription Factors

Y73E7A.1

WormBase description: Is an ortholog of human CCDC124 (coiled-coil domain containing 124). Is predicted to encode a protein with the following domains: Coiled-coil domain-containing protein 124/Oxs1 and High mobility group box domain superfamily.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: TCCGCTCAACGCTCGTCTTC

sgRNA2: ATTATGGTCCATATGTATTT Y73E7A.1-CKIsg3: TTTGCTTCAATTGCGACAGG Y73E7A.1-CKIsg4: GCCAGAAGACGAGCGTTGAG

5'HR: GCATGCGTGTCTTATGGATAAGAAATCGAAAACAATGTAAAATAAATGATACATATTCTGCAATCTTATGTAGTTTTCGAGTTATACAGATTTTAACAACTCAAGTGAATCAAAACCGAAAGAGCACAAAATGTCAGTACTTCAGGTCGCAAAGCGACCCAGGTCGCTAGGTCGCAGGTCGCAAAGCGACCTAGGTCGCGATGCGACCTAAATGAAAACAGCGACCTAGCGACCTAGGTCGCACTCTTGCCACCCCTATGTGGGAGTGCCTCATTTCGGCTTGATCTACGTTGATCTACAAAGAATGCGGGCGAAGAGACGCAGAGTTCTCAACTGATTTCGCATGGTTAAGAACGTGCTGACGTCACATATTTTTGGGCAAAAAATTCCCGCATTTTTTGTAGATCAAACCGTAATGGGACAACATTTTTCGAATTTTTTTCAGAATTTTAGATTTTCACGCGATTTTTGATCTACTAAATGTTAATTATTAAAAATAATTTTCTATGCAATTTTCAGCGATTTTTTTTTCAAAATTTTTGAAAATTAAACTCAATAGACTTTTCATTAAAATTTATTATTACAATTTTCTATTTCTAAAAAAAATATTTTTGTTCAATTTTAAAATAATTTTTGAAAAAAAACATCGAAAATTCCGAAATTCGAGAATTTTCTGCGGTTTTCTGCGATTTTTCCATTTTTTTCTAAATTAAGAGTAAAAAAAACATAAAATTTCAGTAAATTTCCCCAAAAATCCCTTAAATTCCCCATTTTCCAGGCCTTCGAAGAAGCTCGGCTCCCCGAGCTAAAACTGAATCATCCAACGTTTCGTCTGTCGCAATTGAAGCAAATTCTCAAGAAAGAATGGCAAAAATCGCCGGAAAATCCGCTCAACGCTCGCCTTCTCGCTCTCAACTCCGGTACC

3'HR: CTGCAGATACATATGGACCATAATTTCCTATTTGCCCCTCGGTTTCGGTTTCTCGTCAATTTTTTTTCTTGGAATTTTATGGGTTTCTGTGTAGTTTTCCTGTTGTGAAAATGCATAAATAAATATATATAGAATGTTTGAAATATAGCATTTTTAGCAAGCATTTTAGTTCTGGAGCAGGGGTGGGCGGCAAATTGAGTTGTCGACAAATTCGGCAAATTGCCGGTTTGCCGATTTGCCGGAAATTTTCAATTCCGGCAATTTGTCGATTTGCCGATTTGCCGGAAATTTTCATTTTCGGCAAATTGCCGATTTGCCGGAAAATTTCAATTCCGGCAGTTTGTCGATTTACCGGAAATTTTCAATCGATCTTACAGGAAACAGTGAACGCTTACGAACTCATTTCGTGTCTCATTTCGGTTTGATCTACGTAGATCTACAAAAAAAGCGGGAGTTCTCAACTGATTTCGTATGGTTAAGAACGTTCTGGCGTCACATTTTTTGGGCAATATATTCCCGCATTTTTTGTAGATCAAACCGTAATGGGACAACCTGACACCACGTGAATATTCAGAATGTTTATTTTTTCTAATATTATAGAAAAAACACGATCGCACAAAAATAAACAACGACCCAACCAACGACACTCCGCTGTTTTAAGCCTCGCGGCCGACGCATTTCTGTGCAAATGAAATCGTGGGCGGGCCTTTAAAATAGCGGAGTGTCGTTGAAAAAAAAACAAAATTGGAAAAAAAATTTCGCGCTGAAATATTTACAAAAAATTTTGGCGCTAATTTCAAATCTACCGAGAGAATTTGCACAAAATTCAAATTTGTATTTTTAAGACGAATTTTAAAATAAAAAAAAAATTTGGCGCAAAAATATTTTCACGCGGTTCTAGCTTCCCTCATAAACTGAAATGGAATAGTTACTAGT

KI verification primers: Y73E7A.1-CKIF: 5'-CACAGAACAAAGCATTGATTTCTACA-3' Y73E7A.1-CKIR: 5'-TAGTTTTACAACTAAAATCGAGCCG-3' Y73E7A.1-CKIF2: 5'-AACAATTTTAGTCAGCAGCAAATCC-3' Y73E7A.1-CKIR2: 5'-TTCGTAGTTCTGGCCTTTCTCATC-3'

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Strain name: SYS714

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; Y73E7A.1 (devKi233 [Y73E7A.1::mNeonGreen]) I

Expression pattern: Not expressed in nucleus

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): DAF-3, SKN-1, MBF-1, UNC-39, Y56A3A.28, HLH-30, LIN-32, CEH-32, SPTF-1, NFI-1, EGL-44, ZIP-8, ELT-6, ZTF-11

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: SPTF-1, CEH-14, CEH-31, CEH-34, CEH-43, ELT-2, LSY-2, MEP-1, NFYA-1, PHA-4, TBX-2, UNC-42, SKN-1, ZTF-11, BLMP-1, CEH-82, CEH-90, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, LIN-13, SWSN-7, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data