CEH-32

WormBase ID: WBGene00000453

WormBase description: Is an ortholog of human SIX3 (SIX homeobox 3) and SIX6 (SIX homeobox 6). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and transcription regulatory region sequence-specific DNA binding activity. Is involved in post-embryonic animal morphogenesis. Localizes to nucleus. Is expressed in anterior hypodermis; gonadal sheath cell; head ganglion; intestine; and nerve ring. Human ortholog(s) of this gene are implicated in branchiootorenal syndrome 2; holoprosencephaly 2; and microphthalmia.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACTCGCAAATGCAACAGGTCT-3'

Strain name: SYS85

Cross: OP516 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs516 (ceh-32::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-8, MBF-1, UNC-39, PAG-3, CEH-10, TAB-1, EGL-13, CEH-5, PROS-1, CEH-41, SOX-2, SEM-2, HLH-30, LIN-32, LIM-7, Y73E7A.1, CEH-31, Y56A3A.28, SPTF-1, REF-2, NHR-67, CEH-43, CEH-48, EGL-44, FKH-2, HLH-2, ELT-6, ALR-1, ZTF-11, CND-1, MLS-2, NGN-1, HLH-3, NFI-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, LSY-2, NFYA-1, UNC-42, ZIP-8, CEH-8, FKH-10

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2, NFYA-1, UNC-42, CEH-8, FKH-10