WormBase ID: WBGene00001186
WormBase description: Is an ortholog of several human genes including GATA1 (GATA binding protein 1); GATA2 (GATA binding protein 2); and GATA5 (GATA binding protein 5). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in several processes, including cell fate specification; nematode larval development; and positive regulation of nematode male tail tip morphogenesis. Localizes to nucleus. Is expressed in several structures, including ABprpppppaa; body wall muscle cell from MS lineage; nervous system; seam cell; and vulva. Human ortholog(s) of this gene are implicated in several diseases, including Down syndrome; congenital heart disease (multiple); and hematologic cancer (multiple).
|
|
KI information: mNeonGreen knockin at C-terminus
sgRNA1: GCAAATCTCGCAATTCACGC
sgRNA2: GTGCATAAAAAGGATGTGAA
5'HR: GCATGCGGAAGACCACCACCACCCCTATTGTTGTCTCTATTGTGTGTGCAAATTGAGAAGAACGTTCCGCCCTTAGATGGATGTGTGTGTGTGTATCTAATATGGCCAAGGTTATGAAATTGGGAGAAATGATAATTGGCGGTGCAGGAAACGGGACGATCACGACCGGACAGACCAGATGTCTGATTAGGAGACAGGTGGATCCAAGAATCTGACTTAGGATCCGGTGAGGCTATCTAGGACAGAAGCTTGGTGATAATGGAGATCCCAACGAATTCTTTGATATGCTAGAACAACCGGAAGTCTTTAGATCGAAGCTTAAGCCAAAGGAGACCTCCGATCCTTCCGATCCTTCCATCGCTAGGATCCCCTACAGCCTCTAGTAAGAACATTCACATCATCTGCGGATCCTTCTAAAATTTACCAGCGCGAAGATCTATCCGAAGGATCTTTCCACCGATATCCTGAGATCTGAATTTTCTGGGAAAATCTACAATTCTACAGAATCCTTTGATAAGATAATTCTGACCCTTATGCTGCTTGGATTTCTGCGCGATCGATTGTGGCGATCAACCACACTGGGATACTTAGGATCTTAAGGATCCTCACAGTACCCCCCCCCCCCCTCTTAGAAGCTCCTAAATGCCTAAGGATCCTTCCAAAAGCTCACCCAGATCCTTCCCACCTTAACAACTAATTCATTTTCCAATTTCCAGGTTCGCCGACCAATAGAAATGCGCAAAAACCACATCCAACAACGCTACCGCCGAAAGAACAAGGAAAAAGAGTCGTCGGCGGCAACACAGATCTTCAACCAACTCCTCACACAAATGCCGACGATGGCGACCGGCGGAGTGAGCACTGATGGAGCCATCAACACATTCAATTTACTCGAGCAAATCTCGCAATTCACGCAAGCTCAAGAGCTCATGAATAGCAGTGCCACATTTGGTACC
3'HR: CTGCAGTTATCATTATTATTTTTTCCCGCTTCACATCCTTTTTATGCACAATACCTTTATGTAATTTTTCTTATTTATTGAAATTTGAAATTTGAAATTCTCCATAATGTATTATTGATTAATAAGCAATCCTTCATCATTCCATTACCCCTTCAGAAAAATACAGTATTAAATAATTTTCCGCCAATTTTCTAATAATAATTGTGCCCCATTTCCCCCAACTTCACCGCGTGTTTCCGACTGTGAGCTTTACCTCCTCCTCCGACAACTTATAATAATAGTTAGGCTCCGCCCCATTCTCCAGGAGGCCCCTCCCCCAATTCTCTGTGCATGGTTGTGTTATATTCACCTCCTCCCACCACTTTGTCATTTCACTTGAATCATCATTTTTGCATTTTCATATTTATTTTGAAAAAATCTCATCTCACTCTCTCTCTCTCTCCTTATCTCTTGTGGTTTTTGACGGATAAACGTATGTATCTCATTCTATCACTTTTAGCTTTTAACTTCGTCCTCTCGCTCTCTCGCACTCTCCCCTCGCTCCACAGTCTCTCTCCGTCTCTCTCTCTCTCTCGCTCTCCACTTCTTCTTCTTCTAAAACAACGTCACACGTGATTTACAGATGACGTCGTCGAAGGAGGAGATGCCGGATGAGATGGTGAGCCGGGAGGTGGAGGAGGAGATGGAGGATCGGGTTGAGGAGACGGCGACTACAGCAGCAGCGTGCACCGGATGCGCTCAGCTTCACAAGGATATTCGACAGGTGCATATTATTTTGTTTAGCAGTAGGCTCACGCGCCTGCCTACTCTCGACATTTGGCTCCAGCTTCTTGCCCAAGTTTGGCCTAAAAAATATTAACTTGGATAAGAAGCTGGCTAAACTTGGGCAAAAGTTGGGCAAGACTTTGGCAAAACACTAGT
KI verification primers: EGL-18-CKIF: 5'-TTTTGCTGATACACGTACACTCACTC-3' EGL-18-CKIR: 5'-AGTTATACTTGGGTAGAAGTCTGACCAG-3' Download
|
Strain name: SYS673 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; egl-18 (devKi210 [egl-18::mNeonGreen]) IV
|
Expression pattern: Transient-expression
|
Lineage expression pattern
350 stage
|
600 stage
 |
3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-14, CEH-19, CEH-31, CEH-34, CEH-36, CEH-43, CEH-48, CEH-6, CEH-83, CHE-1, CND-1, COG-1, DVE-1, ELT-1, ELT-2, FKH-6, HAM-2, HLH-1, HLH-15, HLH-8, HMG-4, LIN-32, LSY-2, M03D4.4, MED-1, MLS-2, NFYA-1, PHA-2, PHA-4, REF-2, RNT-1, SOX-4, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-62, UNC-86, BLMP-1, DSC-1, EFL-3, ELT-3, FKH-4, FKH-8, IRX-1, MEP-1, NHR-85, SWSN-7, ZTF-11 |
|
 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-83, COG-1, ELT-1, HAM-2, RNT-1, TBX-2, BLMP-1, EFL-3, IRX-1, NHR-85
|