CEH-17

WormBase ID: WBGene00000440

WormBase description: Is an ortholog of human PHOX2A (paired like homeobox 2A). Exhibits DNA-binding transcription activator activity, RNA polymerase II-specific. Is involved in axon guidance; positive regulation of transcription by RNA polymerase II; and regulation of axon extension. Localizes to nucleus. Is expressed in DA8; head muscle; head neurons; and somatic nervous system. Human ortholog(s) of this gene are implicated in congenital central hypoventilation syndrome and congenital fibrosis of the extraocular muscles.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: CTATTTTTCAAGTAGCGCCG

sgRNA2: TCTGTTCCAGGCGATGATGA

5'HR: GCATGCCTGCTGTTTTACATCGAAAATTGATTGCAATGAAAAAAGAAGTAGGTAGAACACCAGAATTAACGCCAAAATTCAAAAACTAGGCGACAGGCAGGCAGACATGAGGCCTAGCTGGCAATAATACCTGATTTTTAATAAAGACTGGCAGCTACCTAGGTCCTGCTACGGGCAAAATATTTCAGCTATCGTACCCGCGAGAATTTTGCCAGATTTTATATTTTTGAAAAAAGCTAGATTTAAGAACCGCAATTATTTTTAAAATTCTGGCAACCAGGGGAATTTGAAATTAAGCATATTATTAAGTTCAGAAAGTGAGAAAATTATAAACTAAAATTTTTCAATTTTTTTTTTAAACTACAGAAGTTCGCTTTTGGAAAATTGAAAAAAAAAGACTTTGAACAAAATTAAAATTGCTTAAATTTTAAAAAAGCTTGCACATTTTTTTGCAAAAAAAACGCTGTCGAGAATCCTTCCTACAGTACCCCCTAGCTCCTCCCACTTTGTGGCTCACTTCTGGAGCTCCAACCCCGCCCTCGAGTCTTGCCGGATCTGTTTTGGATAATCCCGTTTTATTGTCAATTTTGTGTCGTTAACCAATTTTTCCGGTGCCGTTATAAAATTTGAAGATTTTAAATAAATTTTCGATGAATTCAGATAAATTTCAAACCGAATCAATGAAATTAAGAAAAAATTGCACACGAGCAATATATTCTGTTTTAATGTTATATTTAATCGAAGAGAATAACTTATGAATTTGAGCATAGAGCATAGAGGATTAACCTTAACTCTGACTCTACTCAAATACCTGTAAAGTGTCGGCTGCTTCAGCACAACTTGTGATATACCGAATAATTATTTCCTTTCCCTCTATTAATATAACTTCTTGTTTAACACTTCTGAATTTATCAATCTGTTCCAGGCGGGTACC

3'HR: CTGCAGATGATGATGGAATACGGTGGCTATTTTTCAAGTAGTGCCGTAGCTCAACAATCAGGCGATGTTCCAACAACTGCACCATCTGCCGTTACAAATTCATTTTTCTATACACCACAGTCACATAATATTTATCACCAATATGCAACTCCGTATCTACAAAGTGGACGGTGAGATTTTTTTCCAGTTTTCTCCCTGTTATACTAAGCCACTTTTTTCAGTGCCTTGACAACTGCTCACAATACTTCAAGCTCTTCAGCGGGCAATTCCACATCTTCATCATCTTCCTCATCAAACTACCGTAATACAACACACGATTCACTACAAGCTTTTTTTAATACTGGTCTACAATATCAGTTGTATCAAAAATCACAATTAATTGGAAGTGATACGATTCAGAGGACAAGTAGCAATGTTTTGAACGGACTGCCAAGAAGTTCTCTTGTTGGTGAGTGAAAAAATCGGGGTATAGGCTCCAATTAGTCTTGACCGGCTGTTCTACTTTGACAGCCTATATCTAGATTGCATATAAATTGTGTGCAAAACGCGGAGATGGCGGCTGCTGGAAAACATAATTTCACAATTTTTGTGCTTAATTTCTTTTTTTCTTATTATCAAGTATGAAAATATGGAAACATATATTTTACTTTAAAAAACCTTTTGATGAAACAAAGATAACCGAGAAAAAGGTTGTCAAAGTGGAGTAAAGGGGGTCCAAGACTAATTCGAAAAAAAAACTTTTCCAGGTGCTCTCTGCTCAACCGGTGGAGCTCCTTTGAACCCTGCCGAGCGTCGAAAACAACGCCGAATCCGTACCACATTCACATCAGGACAACTGAAGGAGTTGGAAAGATCATTCTGTGAAACTCATTATCCAGATATTTATACACGAGAAGAAATTGCAATGAGAATTGATTTAACTGAAGCCCGAGTTCAAGTGTGGTTTCAGAATCGGCGGGCGAAACTAGT

KI verification primers: CEH-17-NKIF: 5'-GCTCTAATACCAGTTGGATACAATATGAC-3' CEH-17-NKIR: 5'-AGGAAGAGATAAATAGAGATCACAAGCA-3'

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Strain name: SYS608

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-17 (devKi180 [mNeonGreen::ceh-17]) I

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): ZAG-1, NGN-1, CEH-27


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-42

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-42