Single-Cell Protein Expression Atlas of Transcription Factors

WormBase ID: WBGene00003180

WormBase description: Exhibits sequence-specific DNA binding activity. Is involved in endodermal cell fate specification and pharynx development. Localizes to nucleus. Is expressed in several structures, including MSaaaap; MSaapap; MSappp; MSppapa; and MSppapp.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AGGCCATAGTGCTAGAGAGA

sgRNA2: GACATTTTCAGCATTGAAAA

5'HR: GCATGCTTTTAAACATTGGTTCAACATTATCGTATTCATTTGTTTTGCGCGGCAAAAATTTGATCTTCATCTTCCTAATACTACTTTACTCAAAAATAAAATTTTTAAGCGATATAACGTTTTACAAGATTGTAATGCGCCAAACCCGTTTTGAAATTTACCGACAAAATAACGACATTTTCAAAAATTTTGAAAACGTAGTAAGTTGAAAAAATTGCTCGTTTTTATACTTCTAGAAAACGTTTTTTTAGCTTGAAAATATGCCAAAGTACGTGAAACTGTTGAAATTCACTAATATTCCCAAATTCGAAGATCATTTTAGATGGTTATGAAAATCTCATTATGACAACAAACAAGTCTACTTTTGCTGTAGGTATGAAGCAGGCGTAGGCTTTCTTGAAAACATGCAGGATCGCTGTCTTTGCTTTTGCTTTTGGCCGAACATTGAAAAATCACAACCATCATCACTTGTTTAACAAGCAGGTTGATTAAGTTTTTTAAGATCGTTTTTCAAACTTCTAATTCTTCAGATCCTGAAACAGAAGCTGTAGAAAAAAACTTAAGCACTTTATTCATATACAATTTTTGGGAAATTTATATATCAATTTAATTTTCAATTTCCAAATAATCAGACAATACTTGAGTTGCGTTGATATGATTCCTTTTCTCCGCGTAAAATCCATGTTGTGTTCCGTCCGAAGGCAAATAAAAGGTTTCTATTGTAGACGTTGTCTGAAACATGTCCTGATTTCTCCTGCTCTTCTATAAAAATGTTAACATCTGCACCTATTGCACAATGAGCCCATCTTCACCTACATTATCTGATAATAGGTGAAGAAGGACCACCTTGCACCTCTGTCATCATGATGATTTTTGGAGCATTATCATCATTTCTAGTGGGTGTGGTCTACAGTAAAAGATCCCAAAGATTATTAGTTTGAATCAGTTTTCATACTTTATTCCTTCTCTCTAGCACTGGTACC

3'HR: CTGCAGATGGCCTACCCTTACCCTGTTTTCAACGCTGAAAATGTCTTTGACAATACCCAGCAACAAGTTGGATTCTATGATTATTCAACTCCATTCAATGGTACATACTCCTTTACTACTGACTATTCTTATTATAATAACTACTATGACTATGTCAATACTTATGCTTCTTATTATCCAACTGCAATGGATTCTTCATCTTTAAATATTTCATCAACTACTGGAAGTCCTAATTCTTCACATTTCACCACGTTTACTCATTTTTCCACTCCATCTACATCTCCATCTACCTCTACTCAAAGTTCAACTACACCGAGCAATTCTGATAATAAGAAGTCATTTCAATGCTCAAATTGTTCAGTAACTGAAACAATTCGTTGGAGGAATATTCGATCAAAGGAAGGAATTCAATGTAATGCTTGTTTCATTTATCAACGGAAATATAATAAGACTCGTCCAGTCACTGCCGTTAACAAATATCAAAAAAGAAAATTAAAGGTACAGGAAACGAATGGTGTAGATTCATTTTAATGTAAATTACATCATTGTAAATTGTATAACTCAATTGTAATTTTATTGTTTTAAACCGGTAAAATGTTTTTAATTAATATGATTTCATAACTAATACATTTATATTTAATTCTTGTTCTCGATTTTAATTACATCATAACTCAAGATTTATAGAGGAATAAATTTAAAATTAACAAACCCTGTATCGTTTTGAACTTTTGTTCTTTGTTTTCCCAACAGCGCTGTTCATTTTTTTCAATCTCGTCAGCCAGAACAGCTTTATATATGCTTAGGATTTAGAGAATCCGACAGCGGAAACCAAACTTTTGCAGAAAAAAATTGTGGACGGGAGAGCGAGACAAAAAGGGCCGAAATGTGTTCTTCAGTCATTGCTTTGAAAGGTTTCACTTTTCTAGTGAGTTTTGTTTTTCGAAGAACTTTTTGAGCTGCACTATAATTCTTGTATTAGATGTTTGACTAGT

KI verification primers: MED-1-NKIF: 5'-ATGTACAGATGTTGATATGGTTCGTG-3' MED-1-NKIR: 5'-CGCATGGTCACAGTACACATCA-3' MED-1-NKIF2: 5'-CGTGCGTAACAAAATTAATACTCTTCA-3' MED-1-NKIR2: 5'-TTGGGTTGATTGTGACAATAAACAC-3'

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Strain name: SYS433

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; med-1 (devKi75 [mNeonGreen::med-1]) X

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CRH-2, EGL-18, ETS-5, HBL-1, K09A11.1, LIM-7, NHR-102

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CRH-2, ETS-5

Targeted by: NA