WormBase ID: WBGene00002989
WormBase description: Is an ortholog of human ISL2 (ISL LIM homeobox 2). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is involved in several processes, including gonad morphogenesis; head morphogenesis; and pharynx development. Localizes to nucleus. Is expressed in several structures, including body wall musculature; gonadal sheath cell; intestine; rectum; and somatic nervous system.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: AGGCACAAGTACTTGGTATC
sgRNA2: CAATTCTCACTGACATCTCC
5'HR: GCATGCAAAATATTCATCAATGAACATAATACTTTAAACCTCTGAGAGAACCAGTCAGTTTCTGATTTAAATATCAGAAAAATGATTGTGTTAAAGATCGAAGCAAACATACCACATTTTTTCAGTATTGCAGCCATTTTTTGGAAAAGTTACATGAATAATTTATAGACTCTGAAGATGTTCTACACTTTGTGAGTTTATTGTCTTAAACTGTAGTACGCGGAGATATTAGTATGGAAGCTAAAGGAAACTAGAAAAACATTTGAAAATGTACGAGAAAATTTGACTAAAATATAGGATGGATTTTGAACTCTAAAATCTAGGGTTATGAAATTTAAACGTCCTAAGCGATTTGTAATTTAATTTTGAAACTTTAATTGTGAAACTAAAGTGAGGTCAAATTTCTCACATTACTTTCCTTAATTCTTAATTTTTTCAGGAGCAGACAATTTTGTTCAAAAATTTCGAAATTTTCTAAATGTTTAAATATAATTTTTTGCTCATTACTTCAATCTTGATGAGTAATTTCATAGAATATATTTTGCCAGTAACATTTAACTGTCTCAAAAATTTTTTTGGCATTTGAAATGTCATTTTTTAAACTTTTTCATGATCCAAATAGTTCACACAAAACTGCTTTAATCCAATTAATTACAAATAAAAGAAATATAGGTTACCATATCCACAAAATCAGGATGTTCACACTCTTATTCTATCACTTGGGTGTTCACATCCGGCTGGTTTTCTTCTCACTTCTTTCGTTCTCCTCACTTAGTAGGTGGAGTCTACCAACCAGTCGCTCGTCGTCCGCCCGCTCAATGACTCCGCCCACTTTCTTGTCTCTCCGTGGGTCCCAGGGCTCTCGGTTCTCTTTTCGTTTGTGACACTTCCAACTGTCCTATCTGTGTTTGTGTGTATCTAGTAATCAATCACAAACTTCTATATCTGTTCAACGGCGGTACC
3'HR: CTGCAGATGAACATCTGTATGCGAAATGGTTATGAGCAATTCTCGCTCACTTCCCCAGGAACATCGGATCTCGAAATTGGTGGATCATTTTGGAAAGACGAACCGGACACTAAATATTTGTGCCTTGATTCTCCTGTTGAACGTGAGTGTTTTTTTTTTAATTTGAAAATGTTAAGCGTGGGCCAAAATATTTCAAAGAATTTACATCCGGAAAAAATTTTTTTGTAAATTTTCTGAAAATCACAGCTTAAAAAATTCAAAACAAAAGTTAAGTTAGATTCATAAAATTTCCATTTGAAAAGACATCTAACAGATCTAAGAATACAACACCTTCTCTCAAACTCCACTTAATCTTGAAAAATAAATCTTAAAATGTCAAAATCCCTGCAATAACAAAATCGAAAGAAGGAGAGGGAGAAAATCTGGAAAAAAAGATTATTCGCACTTCTAAGCCTTAAATATCCGTCTATTTTCCCCTGAATTTTGCAACTTTTTTATCTGAAGCTCTGGTGCTCTTCCGATTCTTTTCCCATACTACTTATAGGGATGAGCCAATGAATAAATGAGCAAAGTTCCGAAAGGTGTCTGAACTTAATGGGCTCACTTCCAAAAAATTTTGAAGTCATATAACATTACTAAATTTCTTGGTAGAAAAAACCCATTTGAGCTACACATTTTTCCATGACACCTTGCTTATTCACCAGTTTGGCAAACAAAAGAATCGGGGGGTCTCACACAAAAATATATTTTGTTGATTAAACGATATGATGGGGGTGGGGAACAAATGGGAGGCTCCATCAAAAAGCTCTTAGTTCAATCAAGTTCTGAAAGTTTAAAAGAAAACAAAAATCGCTATTTCTTTCATTCCATTTGTCCCCCTCCTCTTTTACTTTTTTCTTTTCCACCTTCTTCTTGTTGTCTTAAAAACGCATAACTTCATCTGGACTAGT
KI verification primers: LIM-7-NKIF: 5'-TCCACCAGCCAAAGGAAGAAT-3' LIM-7-NKIR: 5'-GTACATGTGATGTGCATAAGGCG-3' Download
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Strain name: SYS473 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lim-7 (devKi125 [mNeonGreen::lim-7]) I
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Expression pattern: Lineage-specific, Pharynx-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): AST-1, Y48G1C.6, TAB-1, CEH-45, DMD-5, MLS-2, CEH-32, CEH-41, UNC-39, SEM-2, TBX-38, HLH-30, PHA-4, CEH-27, SOX-2, HAM-2, TBX-8
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: MLS-2, UNC-39, SEM-2, HLH-30, PHA-4, HAM-2, TBX-8, AHR-1, ALY-1, BCL-11, BED-3, CEH-13, CEH-14, CEH-18, CEH-19, CEH-2, CEH-24, CEH-31, CEH-34, CEH-36, CEH-39, CEH-43, CEH-48, CEH-6, CEH-83, CEH-9, CHE-1, CND-1, COG-1, DMD-4, DVE-1, ELT-2, ELT-3, ETS-4, ETS-7, EYG-1, FAX-1, FKH-6, GMEB-2, HLH-1, HLH-15, HLH-8, HMBX-1, HMG-4, HND-1, K09A11.1, LIM-6, LIN-11, LIN-13, LIN-32, LSY-2, M03D4.4, MEC-3, MED-1, MEP-1, NFYA-1, NHR-12, NHR-232, NPAX-4, ODD-2, PAG-3, PHA-2, POP-1, PQM-1, REF-2, RNT-1, SNAI-1, SOX-4, SPTF-1, SYD-9, TBX-2, TBX-7, TTX-3, UNC-120, UNC-3, UNC-42, UNC-55, UNC-62, UNC-86, ZIP-8, ZTF-11, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, FKH-8, FOS-1, IRX-1, MDL-1, PAX-3, SKN-1, SWSN-7, UNC-130, VAB-15, ZAG-1 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: NFYA-1
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