MEC-3

WormBase ID: WBGene00003167

WormBase description: Is an ortholog of human LHX1 (LIM homeobox 1) and LHX5 (LIM homeobox 5). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II regulatory region sequence-specific DNA binding activity; and transcription factor binding activity. Is involved in several processes, including mechanosensory behavior; negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding activity; and positive regulation of transcription by RNA polymerase II. Localizes to RNA polymerase II transcription factor complex. Is expressed in touch receptor neurons; ventral nerve cord; and vulva.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGGCAGTACACCAGAATACG-3'

Strain name: SYS71

Cross: OP55 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs55 (mec-3::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ARID-1, CEH-9, ETS-5, LIM-7, LIN-1, NHR-102, POP-1, SYD-9, UNC-39

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ARID-1, ETS-5, POP-1, SYD-9

Targeted by: NA