WormBase ID: WBGene00002990
WormBase description: Is an ortholog of human ELK1 (ETS transcription factor ELK1). Exhibits sequence-specific DNA binding activity. Contributes to RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including negative regulation of transcription by RNA polymerase II; oviposition; and vulval development. Localizes to RNA polymerase II transcription repressor complex. Is expressed in vulval precursor cell. Human ortholog(s) of this gene are implicated in acute myeloid leukemia.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TTTAAAGAATCCGTTGACGA
sgRNA2: CAACTTTGTAGTTTGAACTT
5'HR: GCATGCGGGCTCGGCGAGCTTGGCAGCAGTGGTAGGACTCCCGGTCTTGGCGAGAGTCAGGTTGGTTTTTTTCTAAATACGAAATATTTATCGATTTTTTCTCTATTTATATCGAAAAATAATTATTGTTCATTGAAAATTAAAAATTCCCGCTTTTTTCGTGTCGAGACCTCTCACACACTTAGGGCTATGCGCCTTTAAAAACACAGTAACCAAGTTAAATTTTTCTTCACAGTTTGTTCAGAGGAATTTCTTTGAAAATCGTTTGGAAAATGAATCTTACTTTTTAAAAAACATAGAACGTAGCGAGTATAAAAAAAAATTTTTTAGCCAAAAAACCCCTAAAAACTGATTTTCTACTATTTTCGCCAAATTTTTAGTCTTATTTTCTAAATTATTAGTGAAAAATATCTCTCCTGTCTAGGATTTACCCGAAAATAGCCTGATTCCTCTTTGAAAATCGTAAAATCTCTGGAAATCAATAAAAGTCCAGCTGTCGTGGCGAGACCTCCCACAAGGATAGAGGTATGAGCCTTTAAAGCTACCGTACCAATTTTTAATGAATTAAATTCATTTCTGATAATTTTCCAATGGAAATAAGGATAAAAAATGATATAAAATGAATATTGTTAAATTTATCATTTTTGAAAAAAAAATCTCGAATTGAAAGTCAAAAAAAAAATCGAATTTTCAACAATTTTCACCCGGTTTCTCCCTAGTTTTTAACAATTTTTTAAAAAAATTCTCAATGGCTTGCATTTAAACATTCAAAATTTCTATACTTTTCAGGTGTTCCAATTCCCGCCGGTCTCCGCATTCCAGGCCACAAATCCGCTGCTAAACACATTCTCCAACCTTATCAGCCCGATGGCCCCGTTTATGATGCCCCCATCACAGTCGAGTACCTCGTTCAAGTTCCCATCATCGACAGACTCTTTAAAAACACCTACAGTACCCATAAAAATGCCAACTTTGGGTACC
3'HR: CTGCAGTTTGAACTTTGGAGCGAGAAAAAAATAAAAATTTTCGAAAAGCAAATTTTTTTTTTCGGACATTTTTTTCCCATTTTTTTTTGTTAAACTTTGCCATGGGTTTTCGATAGTCTTCTTTTTCCAATTCCCTCTTCATTATTCCAAAAAATCAATCAATATTTTGTATTCTCCATGATCTCCCCCACCGGATGAGAGAGCGAGTCGTGTAAATCTCAATAAAAACTTTAACTTTTCTTTTTTTTTACTTTCCATATATATTTTTTTATTCTGCCCTCAAAATATTCGCAATAATAATATAATTTGTATATTTTGTGCCTTCACCATGTCGTTTATTTGGTTTTTAGTGCATTTTTTACTTGTTTTTACCCCAATTTTTGTGTTGATTTACTAGAAGAAATGAATAATTTGTCAAATACCTGTTTTTATTTCTGAAAAATCTTTGAAATACTACAAAAAAACTGAAGAAAACGGAAAAAATCCAGAAAACCCAAATTTAAACTCCCTCCATTTGAAATGTGTAGTACGCAAACACCGCAGCTGACGACACATGTCGAAACCCAAGATTCTACGACAAATAATTGTTTTCAACGATTTTCAGATAAATTTTTCGTGTTTTTGAGTAAAATTCAGCAATTTTCCAATTTTTTACAGGTAATATTTTATAATGGACGATGAAAATGAGCTATTGGGACCTCCAGATGACCCAGAAATCGAGAAAAATCGAGTTTTATCGATTTTCGGCAAGATTTTATCGAATGAGCCGGTGCAGCAGCTCACAGCGATCGATGTGCAAAATGTTGAGCTTGCGGTAAGCAAAAAAACTCTGAAAATCAACAGAAATGTGAAAAATTGCAAAAAAATGGATGAAAAAAAATGAACTAGT
KI verification primers: LIN-1-CKIF: 5'-CTTTATGGACCACAAATATCACCAC-3' LIN-1-CKIR: 5'-GAAGCAGCCCCGTGGATAT-3' Download
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Strain name: SYS645 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lin-1 (devKi202 [lin-1::mNeonGreen]) IV
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Expression pattern: Lineage-specific, Posterior-biased, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): RNT-1, LIN-22, VAB-15, NOB-1, NHR-23, CEH-16, LIN-39, GMEB-1, CEH-40, CEH-13, CEH-6, LIN-26, CEH-20, NHR-25, UNC-62, BAZ-2, Y56A3A.28, REF-2, ELT-1, HAM-2, PHP-3, ETS-7, HBL-1, SPTF-1, CEBP-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: UNC-62, ELT-1, SPTF-1, ELT-2, HLH-1, MEC-3, UNC-42, UNC-55, RNT-1, VAB-15, BLMP-1, C16A3.4, DPL-1, DSC-1, EFL-1, ELT-3, FKH-4, LSY-2, MEF-2, MEP-1, NHR-85, PQM-1, SKN-1, TBX-2, ZTF-11 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: BLMP-1, DSC-1, ELT-3, FKH-4, MEF-2
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