WormBase ID: WBGene00004096
WormBase description: Is predicted to have nucleic acid binding activity. Is involved in defense response to Gram-negative bacterium; determination of adult lifespan; and innate immune response. Localizes to cytoplasm and nucleus. Is expressed in head muscle; head neurons; and pseudocoelom.
|
|
KI information: GFP knockin at N-terminus
sgRNA1: TAATGATTTCGGCAGTCCGC
sgRNA2: CATAGTAGTCGGCGGTGAAC
5'HR: GCATGCTAGAATGCTTTGGTTTTTTATCTATTTTTTTCAAAGTGTAAGATGCTCCTTCTTCTACAAGTCGAAGTTAAATTTTTTTGATATCTGTAAAATAAACCAAAATTTAACCTATCAAAGTGGAGTAGCTTCTACAAGTCTCTAGTCAGCTGGTTTCAGAGATACACCCACTCTCGGTGATAACGCGCGTTTCCACCAATTTCGTATACAAACCAAAAGTTTTCGAAGAAAGTGAAACGTTGTCAACTGATAACGATTCGAGCAGCGCTTTCCACGACTGACGAAATTCGCCGCCCAACTTTTCGTCATCTTTATTAGTTGCTTTTGGGCAATTGTTTCAGTTTTATTAGTTCTTGGGTTTGTGAGTATTTATATTTGAGTTAAACTTCCCTGCCCGGCCTTGGCGCTTGTCTAAAAACTACCTTGCCTTTTTTTACAGTTTCACGTATTCGCAACAATGTAGAGTACCCACGTTTCAGAATTTTTGCAGTATGCTTTTAAGGTTTAATAAAATCCCTAATCAGTAAAGTATTCTCAAATTGTTTTTGAACTTTGGGCATCTATATACCTCGCGGTCATTATTATTAGACGAAATCATTAAAAATAACTTTCAAACCCACTTCCCATCCTAATTAGATCAAATGGTTCAACTTCTATCCTAGTAGAATTTCCCAATTTCCATTCCCAAATCCCCGAAAAAAAATTCAATCTCTACTCTCATCAGCTTCTGCATTTAGAGTGCTGTGTTCTTGTTCTAATGCGGATATCTACACTTTTCCAGCTAATTCTCGTAAACCCTCGTTTCGCAAACTGATAGTGCTCTCCAATTGCTCTCCCAAAATGTCCCAAAAAAAAAGTTTCTTGTAACTTCTTCCGTCTCTCATTATTTTCGGAAAACAACCCTCAATTTTGCAGGCATAGCTCTCAGCAAGTCTCATTTCCGAATGTCGTTTGGTACC
3'HR: CTGCAGATGTCGTTTTTGAATAATGATTTCGGCAGTCCGCCTGCAACAAGTTCACCGCCGACTACTATGCCTAAATTACCGACGATTCAAGATATGCTTAACAATATTGGTGCATCCACAGGTACTGTAGATTACTGTAGAGGATTTTAAATGTGCATAGTTTGGTAAATAACCAAAAAACATAGCTACTCCGTAATTACTCAGTCAGAATAAACGAAAAACCGAAACATCACAATTTTATTCTAATAATTCATTCATCATTTTTCAGTAAACCTAATGCAGCCGAACCCCTACCTGATGCAAAACCAGATTCCTCTGCCAGTCCCCAACCTACCCTTGAACCCATTCCTCCATCTGAACCCTGCAATCAGTCAAGAAATAATTCAACAATTCATCGCGATGAGCTTTAACACACCAAATGTATTAGCTAGTATCGCCAACATGGGAGATGATGAAGGACCTTCCTGCAATCCAAAAATGCGACGAGGTGATCTGCTGAAGAGTGTTTCAATGGATAGTACAGAGGATCCACCATCCATAACACTTGATAACAATGGAGACATGATTGTTCCAAATAATGATAAAGAAGGTTGGTATATGAAAGGTCTTGACCCTTTGACCCTTATCTTTTTTCAGGTTGGTGTCGCAACAAGAAGTATATCGAACAGACGGAGAACGGTTACATGTGTACTGTTTGTCGAAAGGTTTATGGTAGATACAACTCGGTGTCTTACCACGTTACCATCTACCATAGGAATCCTCCAATCAAGTAAGTGAGGGAAGGAAAGTTATGCCTCCCATTTAAAGGTGCAGAGCAACAAGCAGCCGACATTGATCGGCGCTTTTCTACTGTCGGCTGCAAGCTTACTGTAAAGTTACAACAGATAATATTAAAGGATCTATCATTTCAGATGCAACGTTCCCAACTGTCAGTTCACAACTCGCGAGGCTCGATACATTCATTTCCACAAGAACTATCGACTAGT
KI verification primers: PQM-1-KIF: 5'-TTCCCGCATACCAACTCTTT-3' PQM-1-KIR2: 5'-GAAAAGAGCAGAGACTGGTGAC-3' Download
|
Strain name: SYS573 KI background: RW10226 Strain genotype: itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); pqm-1 (dev60 [gfp::pqm-1]) II
|
Expression pattern: Intestine-specific
|
Lineage expression pattern
350 stage
|
600 stage
 |
3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): MML-1, MDL-1, ELT-2, NHR-232, ELT-7, END-1, NHR-102, ODD-2, DVE-1, NHR-12, DPFF-1, ATF-7, PHA-4, CEH-20, HBL-1, F23B12.7, LAG-1, CEH-74, SEL-8, CRH-2, NHR-10
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: MDL-1, ELT-2, NHR-102, ATFS-1, CEBP-1, CEH-39, DMD-5, EFL-1, ETS-4, ETS-5, FOS-1, GMEB-1, HLH-30, K09A11.1, LIM-7, RCOR-1, SMA-2, SWSN-7, TLP-1, ZFH-2, ZIP-3, MML-1, PHA-4, LAG-1, CRH-2, BCL-11, BLMP-1, ELT-3, ETS-9, HLH-11, HMBX-1, LIN-1, SKN-1, Y55F3AM.14
Targeted by: ELT-2, HLH-30, MDL-1, DSC-1 |
|
 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: ELT-2, ATFS-1, CEBP-1, CEH-39, DMD-5, EFL-1, ETS-4, ETS-5, GMEB-1, HLH-30, RCOR-1, SMA-2, SWSN-7, ZFH-2, ZIP-3, MML-1, MDL-1, PHA-4, LAG-1, CRH-2, BLMP-1, ELT-3, ETS-9, HLH-11, SVH-5
Targeted by: ELT-2, HLH-30, MDL-1, DSC-1
|