Single-Cell Protein Expression Atlas of Transcription Factors

GMEB-1

WormBase description: Is an ortholog of human GMEB1 (glucocorticoid modulatory element binding protein 1). Is predicted to have DNA binding activity and transcription coactivator activity.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ATTCTTCCATCGTCCGTGTC-3'

Strain name: SYS575

Cross: OP343 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs343 (gmeb-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Tissue-specific (Skin and Muscle)

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): RNT-1, LIN-22, C16A3.4, NHR-12, NHR-23, CEH-16, CEH-40, LIN-26, BAZ-2, REF-1, HAM-2, NOB-1, LIN-39, ELT-1, NHR-25, CEH-20, UNC-62, UNC-130, LIN-1, HBL-1, CEH-41, TBX-8, Y56A3A.28, HLH-1, F23B12.7, CEH-13, CEBP-1, T07F8.4, Y75B8A.6, IRX-1, TLP-1, NFI-1, ATF-7, CEH-74, SEL-8, CRH-2, NHR-10, CEH-43

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, CEH-48, CEH-6, COG-1, DMD-4, ELT-2, HLH-30, HMG-4, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PQM-1, REF-2, SOX-4, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, ZTF-11, C16A3.4, BLMP-1, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, FKH-8, FOS-1, LIN-13, LSY-2, MDL-1, NHR-85, SKN-1, SWSN-7, UNC-86, VAB-15

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data