ELT-1

WormBase ID: WBGene00001249

WormBase description: Is an ortholog of human GATA1 (GATA binding protein 1). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding activity. Is involved in several processes, including epidermal cell fate specification; regulation of transcription by RNA polymerase II; and spermatid development. Localizes to nucleus. Is expressed in several structures, including hyp7; hyp7; hyp7; seam cell; and vulval muscle. Human ortholog(s) of this gene are implicated in several diseases, including Down syndrome; congenital heart disease (multiple); and hematologic cancer (multiple).

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TTTCTACTTCAACTCGATCG

sgRNA2: CAAAGTCCACCATCATTGGA

5'HR: GCATGCTTCTTAAGGGATCAGTTTTTTGGCGCTCTACTGAAAAAAATTGGATTTGATTTAAAAACGCTGTTTCAAATTTTAAAGTTGAACGAATGCAGAAAACCATTATGTGATTTGCGCTGTATTACCAAGGGTCAAATTTTTTATAGATTATTCAATAATCTTGACTTTTGGAAAACCACAAAAGGATCTATGCATAGCTATAAAAAATACAGTACACTCCAAACGATCAAAGCTCCTCCTTTTACCGACAAATTGGTTGCGCGAAATAGAGTTGCGTAAGTTTAGTGTCGTCTTTAAACCTTCTTCGCGCTCTTTACCTTATCGTATCATCCCTCCTCCGATATGTAATTACTTCCACCACTACCCTAAGCAAAACATTAATCGCGATGCCGACGCGATAAACGGATTCATCGTTTTTGCTATAAAGTGTCATGATTGATGGGATTTTCCCACGGCATTGTTTTCTTTTGGTCAGTGTCTGATTGGATAATTATACTAAAGGCAATTATATGAGCATCCGGGAAAAGAAAGGAAACTGTTTAGTTTTATGGCGCAACACTATTTAAGTGAGCCGACTGTTTATGATTGTAATGAAGAAGAAGTGAACATTTGATGGTATTCACATCATAAAATGCAAAAATCGATTCAAAAACTGAACATCATTATTTTTCAGGTGGAACGTCCAATCACCATGAAGAAGGATGGAATTCAGACCCGTAATCGCAAGCTCTCCGCAAAGGGATCGCGAAGAATGAAGAAAGAGAATGGCGGAACTCCGACTTCAATGGGAATGCCTACAACATCTTCAAGTATTTCATCTGGAATCGAACTTGATCAAAGTGGAGTTTGGGGAATGAAAAACACTCAACCAATGCTCATGACACCGACAGCATATGCATTCCCAGCATCCAATTTCTACTTCAACTCAATCGAAGATCAATTGGAGTATAAAACGTGTCCTCCAATGATGGTTGACTTTGGAGGACAAATGAAAAATTTGAATTTGAACGGTACC

3'HR: CTGCAGTAAAATATTCATTTTAGCAACTCTTTTTTTTCATTGATTCTCCTCATTTCAAGACATTCCATTCTTCTTCTGCCTCCCAAAATTTCTTTCATCCTCTCATATTTCTGTGATATAAATCATCATCATCTCCACACTTTCACATTGAATATAGAATAATTTGTGACATAATTTATTAATTTTCCTGCGGTTTTCATTACCTCAAACCACGTTTGTTTTTATGTTTTTCCTCAATCTCACAATATGTGTACTATATTTTTGTTCTCGCTACAATCACTTTTCCTGCCCGGCTACTCACCAAAACTCTGAATATTTGGAAGAGTTTGTATGTTTTCCCCCTTTTCAAACACCAAAAAGGTTTCTTGATGTTTTTCTCATTATTTCAAGGGGTAGTTTGTGTTTCCCTTGGATCCCTCGCATGGTCAGAAATGACAACTCTGGTATTTTTGTGAATTTATTTATTGAACATAAAAATTAAAAATTAAACAATTTTATTCATGGAAACCTGAATTTTAGATATTGTTAATAAGGATCAGCAGATTTTCGAGAAAATTTTGCTTAACATTTCTGTAACTTACTCATTAAAACTCATTAAAATGCTCAATAAAAAAATTAGGTTGTCGAATTGAAAGTTTAAACAAAAATAAATGTTTGTATTAGGTTGGTCGAAAAGTCTTTGCAAAATTTTGATTTTTTTTTTGGAAGTAGTATTTTTTCAAAGCGAACAAGATTCAATGGGTAATATATTTACCATTAATTCCTATGACTTCTTGCCAACACAACGGAAGTTGAACAGTACCCTCCGCCTAGAACTCCTGCGATTGGGAGGCAAAGAAGTCGTCCAACCCCGTTTCGACGACCTTTCGATCATGAAACTTCTGCCCGACTAGT

KI verification primers: ELT-1-CKIF: 5'-ATCCAGTGTGCAATGCTTGTG-3' ELT-1-CKIR: 5'-GCTTGGCTCCTACTGACTACCAT-3'

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Strain name: SYS680

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; elt-1 (devKi217 [elt-1::mNeonGreen]) IV

Expression pattern: Lineage-specific, Skin-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIN-22, RNT-1, ELT-3, NHR-23, NHR-12, CEH-16, GMEB-1, LIN-26, NOB-1, BLMP-1, NHR-25, REF-1, CEH-40, TBX-9, CEH-13, HAM-2, VAB-15, LIN-39, UNC-62, LIN-1, TBX-11, UNC-130, T07F8.4, TBX-8, BAZ-2, CEH-41, CEH-74, HLH-14, CEH-83, ETS-7, CEH-43, PHP-3, CEH-20, HBL-1, IRX-1, NFI-1, LIN-32, Y56A3A.28, VAB-3, CEH-39


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: LIN-22, RNT-1, NHR-23, CEH-16, BLMP-1, NHR-25, REF-1, CEH-13, HAM-2, VAB-15, LIN-39, UNC-62, LIN-1, UNC-130, TBX-8, HLH-14, CEH-43, CEH-20, HBL-1, IRX-1, LIN-32, VAB-3, ALR-1, BCL-11, CEH-36, CND-1, CRH-2, DAF-19, DVE-1, EFL-3, EGL-18, EGL-44, HLH-2, HLH-30, LAG-1, MDL-1, NHR-102, NHR-127, NHR-35, NHR-49, POP-1, SEM-2, SEM-4, SKN-1, SMA-2, SWSN-7, TLP-1, ZIP-8, ZTF-6

Targeted by: RNT-1, HAM-2, UNC-62, CEH-43, NFYA-1, UNC-3, UNC-55, BLMP-1, IRX-1, EFL-3, LSY-2, MEP-1, NHR-85

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-23, CEH-16, NHR-25, HAM-2, VAB-15, UNC-62, CEH-43, CEH-20, HBL-1, IRX-1, LIN-32, VAB-3, ALR-1, CND-1, CRH-2, DAF-19, DVE-1, EFL-3, EGL-18, EGL-44, HLH-2, HLH-30, K10B3.5, LAG-1, MDL-1, NHR-127, NHR-49, POP-1, SKN-1, SMA-2, SWSN-7, TLP-1, UNC-37, ZIP-8

Targeted by: RNT-1, HAM-2, UNC-62, CEH-43, NFYA-1, UNC-55, BLMP-1, IRX-1, EFL-3, LSY-2, MEP-1, NHR-85