CEH-41

WormBase ID: WBGene00000462

WormBase description: Is an ortholog of human HINFP (histone H4 transcription factor). Is predicted to have DNA binding activity. Is expressed in body wall musculature; dorsal nerve cord; head muscle; head neurons; and ventral nerve cord.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ACTCACTTCAGGATCGCAAG

sgRNA2: TGGTCCTGAAACAAAATGCT

5'HR: GCATGCGCTCCGCTGGTATTATCTCACAAAATGTAGGCACCTTTTGCATTTCATAGATTTTTGGAGGTGACGGCAAGAGACGCAGAGGAAATGCACTTTGTGATACTCGCTGCCACACATTTGCACACCGGTGGTTATCCTCTCACCTCTGGCCTAAAATTATTTAGGATTTTTAGAATATTCTCTTGAACTTTGAGATTTTTTCGGGACCACTTTTTGGCAACCGAGCATTTGCAAAAAATTGTTGATTGGAGCTCAATGCAGCATCAAATATGGCTCCTGTACTCTCGCAATGGTTTTTTCCAATGCTTCGTGTTTTTTTTCCGAAGGCACTGACTTCAAAATTCGAGTCAAAACGACCCGTTTCGTTGGAGCTCTTTTTGGAAGTCAAAATTCCGTTAGGTAAGTGTTTCTTCGAATTTTATCAATAGTTGAGCTTGTGAGCTCTACAACCTTTTTTACAATCAATTTTTTGACTGGTGGGTGTTCAAAATCATTTTCATCTAGGTCTTGTGAACTCCACATTCAATGAAAAATGTGCTCAGAAAGTCAAATATTTAATTTTTTCGTTTTTAGGATACGGCTTCTGATGTGATGTCATCAAGGCGTCATTGGAATGAGTTAAATTGGCGATAAGTTCAACACAACCAAAATGTACGTGTACCGCGGCTGGAACAGAATCCAGAAATCACCAAGCTTATCGGAACAATAATTGGAAGACATACTTGGTGGAATGCCACCGTTAGAGTCGAGGCGTTGGAGCAGCCATAAATAGATAATCTCAATAATTTTGGTGCTCCCCCAATTCTGATTCCCAATTTATCTATTCAACTCCTCCAAATTAATAAATATTTGAAAACACTTGTAACAATTGGGTTTTTTTTCTTTTTTTCGAACAGTGTTCTTAAGCACGGCGTCCTTATCTTTATATTTTATCTATTTGTATTTTATTTCCATTATCATAATTTCAGGGTACC

3'HR: CTGCAGATGTCTCAGCACTTTGTTTCAGGACCAACTTCCTGCGATCCTGAAGTGAGTCATTTTATCATTATTTTCTGGAAAGTTCTTGCAAGATTTCGTGTAAATTAATATTGTCAAAATGTTTTCTTTTCTAATTTTTTCATCTTAACAAAAATATCCTGAATCAAAAAAACAATGTGAGCTCTAACAAGCATCAGAATTGTGATTATATAAAGTATGTGCTTAATGAGAAAACTCTAACCTTTCTCCAAAGCAATGTGAATGTTATAAACTTCACATTTTCAGAAAAAAGTTGACTTGCCATTTGGCTCGCAGAAAACAATGAGTGGCATCATTCTTCGGGCCGACGTCTCACAGGCCCATAACATTGCACTGCTTATATATGGCCCGGACTATCTGCCTCAAATGGCTCTTAAAGGCGTGGAGGATCCTCATGGAAACGTGGCTCCGTCAGTGATCTCAGAGCCATCAGCTATTGCTCCAGAAGTTTCAGCAACTGATAGTCTTCAAAAAGACACAAAAAGCGATGAAGCTTGTATTGGCAACATGACTACAAATTCCGTAGAAAAATCAGAAGAAGAAGGGGAAAAAAGCGATGCTCAGCCGTCAAACCAAGGAAACCCTTCCAGACAAAAGCGTGAGTTTTAAAAAATTTTTGCAGGTGTACTAACTAATCATTAAACTTTTAATTATAAAACAAAAATAATCAATTTTTTCAGAACCTCCAACTGGCAAGAGAAAGCGGGATGATCTGCCCGCCCGCGAACACTCGGAAACATTTTCAAATGTAGAAGGATCATCGGAACGTTCTACGACAAGATCACAAAGCCGAGTCAGTCTTGACGAGGACCGTCAGAATTCGGCTCCGGTCCGTCGTTCAGTTGAAGCACACATTCTTGTGCAGAATGCGACTCCAACTGTGGTTGGGTAAGTACTTTCAATAAAAATTATTTGAAGCAATCTGACACTAGT

KI verification primers: CEH-41-KIF: 5'-GTTTTACCCCTTTTCTACTATCCATCTC-3' CEH-41-KIR: 5'-TTACTGTTCTAGTCAATGAATAAACAGCTT-3'

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Strain name: SYS425

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-41 (devKi276 [mNeonGreen::ceh-41]) X

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): TAB-1, PAG-3, NHR-67, Y48G1C.6, GMEB-1, Y56A3A.28, SEM-2, CEH-32, UNC-39, TBX-38, HLH-30, REF-2, LIM-7, NHR-25, ELT-1, NFI-1, CEH-43, CEH-83, SOX-2, HAM-2, SPTF-1, ZIP-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA