Y48G1C.6

WormBase ID: WBGene00021679

WormBase description: Is an ortholog of human POGK (pogo transposable element derived with KRAB domain). Is predicted to have DNA binding activity.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TCTGTAGGGATTTCGCTCAT

sgRNA2: ATGTTCAAGGGCATAATTTA

5'HR: GGATCCATGTAGCTGTGAAACACAATCCAAGAAGAGCAACCACGTCGGCATGACGTCAGTTCTCATTTTATTCTGTGGTTTTCCACGTCTCGATGGATCATCGTCGGTTCTTCCGAGTAGCCGTTCACCAAATGGGTGGCCAAGGGCAATCCACATTTTTTCGATGAGTTGTTGAAGACCTGAAATTGATTTTTAAATTGAAAAAAAAATTTCGGAAAATTTTGGAAATTAATACAAGGAGAGAACTAAATTAAAATTAGTTTTTTAAACCCCATTTTTAGTACAGAAAAAACATTTAATAAACTCACCCGTAGTTGTTCTGTAAAATGGATCACAGCACATTTGCGTGAGGCTGGCCACAATAATCGACATATCTGATCCTTCGTCTTCTGCAAAATATCCGGTGTTATAACCATTCAAAAGTACCATCAATTTGTTAGAGATAAACTCAAGATTAATGCATTTTAGTAGAAATCAGTAAGGGAAACTGTAAATCGTAGCCGAGTAGATCGAACTTCTTTCATAATTTTTTTACGATTGACCGGAGGTTTTTTCCATTTCTTGGTATTTTCTTTTCAATTCCAAAAAGTCATTTACATAACTACTTACATACAAATATGTGGGGTGCCCATACACGTATTCAGTCTTACTCTCTTTTCATATAATAATGTAGTTTTTCTATTTGTCTTGTCTCCTCCTATTAGCCTTGCACCCCCTTTACATTCTGACCAGTCGGAAATTCGTCTCACTGCCTCTACCCTCTAATAGTCTTGCACACTCTATTAGCCATGTCTCTCTGCGTGTGTGCCAATTTGCTGTCTCCATTAGTCTTGCCCCTTCGTTCGCTTTTCTCACATGTTTTATTTCCAATAACTTGCTGACTAGTTAAGACTTTGCTTGTTATATACTTATTTTTCAGAGAAAACCAGGTACC

3'HR: CTGCAGATGAGTGAAATCCCTACAGAAGTGCCTGTAAAAACTCGAAAAAGAACGTATGACTTGAAGTTCAAACTGCATGCTATAAACTATGCCCTTGAACATAAGAATATATCGAAAGCAGCAAAGGATTTGAATGTGAATAGGCAAAACATTCAACAGTGGATCGCCCAGAAAGCTGAAATCGAGAAGCAGTTGTAAGTTTTGCCACTTCGGACATATTTTTGTGTTCAAAAAAATAGTCCAGGGCTAGGACTTTCATGTTAGGCATGCGCGGTGTCAGGGTCCTGCTTGAAACCCGCCTATGTGAATTTACAAATTTCTAATTTTCTCATTTCTCTCAATTTGATGAAAATCAAAATAGAAATTAAAAAAAAAAAGATTGATTATGTTTTAGCACAACGTAAGGCGTAACCGAGAGGCAAGCGGAGGTCGCCTTAAAGGCAGGGTTTAACGCCTACATGCAAGCCCTATCAATTGAGAACCTTTTATGGACCTTTTATTCCAAATACTATGATTTTTTCTAGGCTTATAATTAATCCTATCAAAAGAAGTCATTTCAACTATTCCTATATTTTGAAATACGGGCATAACATAAATTCTCTGTTCGATTTCAAATTTTCTTTTTGAAAAATTTTCGACTTGCTGCCTTTTGGCAAATAACACTTTCAGAGAAGACAACGGCTCAACTGCAACAAAGCGACTTTCGGGAGGTGGAAGACCTTTAAAGTATGACGACGTTGATAAGGAATTGATTAAATGGGTTCACGATCAGCGTAAGGAGAAACAACGAGTCACCAGAAGAATCATCGGTGAAACGGCAAAGAAAATCAGTCAGAACTCGGATTTCAAGGTAGGAGAGCCGTTACTTTAATGCAAACGAGTATATGAAAAACATAAAAAATAACTATTCGACGCTACTCCACCTTTATCATTCAGGCATCCCGCGGATGGTTGGACACTAGT

KI verification primers: Y48G1C.6-NKIF: 5'-ACCAAACCTACTCAAATGTATATATTCG-3' Y48G1C.6-NKIR: 5'-TCCGTTCTTGCTCGTCGAGTA-3'

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Strain name: SYS485

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; Y48G1C.6 (devKi132 [mNeonGreen::Y48G1C.6]) I

Expression pattern: Lineage-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): AHA-1, CEH-2, PHA-2, PAX-1, CEH-5, DMD-5, AST-1, PAG-3, CEH-16, EYG-1, CEH-45, MLS-2, CEH-34, LIM-7, CEH-41, TAB-1, Y56A3A.28, BAZ-2, HAM-2, UNC-39, PHA-4, TBX-2, CEH-27, Y75B8A.6, SOX-2, CRH-2, CES-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: HAM-2, CEH-83