CEH-45

WormBase ID: WBGene00022837

WormBase description: Is an ortholog of human GSC (goosecoid homeobox). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is expressed in pharynx and spermatheca. Human ortholog(s) of this gene are implicated in several diseases, including WAGR syndrome; diabetes mellitus (multiple); and eye disease (multiple).

KI information: mNeonGreen knockin at C-terminus

sgRNA1: GCTGTGCGATGTCTTCAGTG

sgRNA2: ATAAAACGCGAAAATTCCAG

5'HR: GCATGCGAAATCCACGAAAGTGGGTTTTCCCGGATTTCAACTACCCGGGTTTTTACAGATTTCAAAGTTATTTTTTCGCAAAAAAAAAAACAATACCCGAACATTCCGGGTTAATGAGTCCATTAAACCCTATAATACCATATAGCTGTGTTTCTGAGGAAAACCGTCTGTGGGGTAATCTCTAATTACAACCTCTCACCTGCCTAAATCGTGTCGGCCCTTTTCAATTATTTCATCACCACCAACAGCTCTTCTTCTTCCGTTTATCTTCTTCCACCTGCCTCACTCACTTAACCCGGCAAATCTCAACCTGTAGAGGATGAGGGGGGCGCTGGAAGAGGAGTGACGTCGTCATAGTTCGGCCGCCGGCGCTGGCTTGATCTACGTAGATCTACAAGAAATGTGGCAGAGTTTTTGACTGATTTTGTATGGTTAAGAACGTGCTGACGTCACATAATTTTGGGTAAAAAATTCCCACATTTTTTGTAGATCAAATTGTAATGGGGCAGCCTAGCACCACGTGCCTTATCAATTGAATAATCGATTTTTAAAATAATATTCTTTAAATAAATTTTCTCTTCCAGGTCTGGTTCAAAAATCGTCGGGCAAAAGAGCGCAAGCAGAAGAAGGACGACAGTCGGACTTCTAAGGTAAGCTAGTACACTTTTTGAAAATTAATTTTCAAATGCGCTCTACTGTACTCTCACGGTCTCCCTCCATCACCGCCAAGAACGGTAAAATTGGCTCAGTGGTAGGCGCGGTTTTTTGTGTCAACAGTTTTTGGTTCAATACCCAATAGTGATTTTTTTTTACCTAAATTTCTCTACATTTTCAGCACAGCGGAGACGATTCCGAATGTGACGAGAGCGACGAGGACACCCGAAAAGTGAAACGGATAAAACGTGAGAACTCTAGCGGAAAAGAGACGAGCTCGCCCGAATCAAAAGCTTCACTCAAGACATCGCA CAGCGAGAATTTGGTGTTGGAGCCTTGTGGTACC

3'HR: CTGCAGCATATAATTTGAATCTCTATTCCCCACCTAGCTGGAAATCCAGGCCACCCCCTCTTTATATTCTAGTCCCCCCCATCCCCTCTCATTTTGTCAAGTCAATAAATGTATGTGGCGAATTAATCCGATTCTAATCACGTTATCATTTTGTTGTGGTGGTTGGTTACTGTAGATAATCCCCCGTAAAATTCGCACCGCCCGACCGGTCTCTGGCGGATTACTGTAGCCGTTTGGGGGGAGAGGGGGGTTTGTAAGGAAAATCACATTGCTAAATTAATCGAATTAGCATTTTTATCAGCAACGATTTATGCGTGTGTGTGGAAGGTATTACTGTTTTAAAGAGGAAGTACGGTATTAAGATACCTACCCTGAAGGTAATCATGTGGAAATTTGATGATGTTCTCAACAGAGCGCGTTTGCACACCGCGGTGTGACACCTAGCTGTCTCCAGGCTCCCCTACGCCTCTCGCACCTGCCGCCGACCATTGGTGCGGCTCATGGCGCTGATTTAAGGCACCAGTCCCTTGTTCGGGGGTTCCACTCTCTTCAGTTTACTTTTGCATCGATGCACCAATCTGCAAATCCGGTCAAAATCCGCCGCACTAATCCCACCCGCTGCCGAAGGTCCCAAATCTTTTGCAAATTCTCGTGGGAAACGTTGCGAAGTCATTTTAAGAGGCTAATCCAATGTTTTCCAAAGAAATTAGAATTATTTCTGAATTATGAATGACAGAGAGGAAAGCAGATTCCAATTTTCGCTGTTAGAAAAAATATATGAGCGGGGTGTGGAAAAAGACACGGGCTCAGAAAGCACGGAAGCTCAAAGTGTCCCATTTTGGTTTGATCTTTGATCTTCGAAAAATTCGGGAAAAGAGACGCAGACTTTTCAACTGATTTCGCATGGTTACTAGT

KI verification primers: CEH-45-CKIF: 5'-ACCGAAACCCCATAGAAATTGA-3' CEH-45-CKIR: 5'-AGAGCTAACGAATTATGATTTATCGG-3'

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Strain name: SYS634

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-45 (devKi191 [ceh-45::mNeonGreen]) I

Expression pattern: Lineage-specific, Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-2, AST-1, DMD-5, PAX-1, Y48G1C.6, EYG-1, SEM-2, CEH-34, CEH-16, PHA-4, LIM-7, CEH-27, MLS-2, DVE-1, M03D4.4, SPTF-1, Y75B8A.6, CES-2, SOX-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-34, CEH-36, LSY-2, NFYA-1, REF-2, ZIP-8, ELT-3

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, LSY-2, NFYA-1