CEH-36

WormBase ID: WBGene00000457

WormBase description: Is an ortholog of several human genes including OTX2 (orthodenticle homeobox 2); PROP1 (PROP paired-like homeobox 1); and RHOXF1 (Rhox homeobox family member 1). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in neuron fate specification; positive regulation of transcription by RNA polymerase II; and sensory perception of chemical stimulus. Localizes to nucleus. Is expressed in ABaraappa; ABaraappaa; amphid neurons; and head neurons. Human ortholog(s) of this gene are implicated in several diseases, including Leri-Weill dyschondrosteosis; diabetes mellitus (multiple); and eye disease (multiple).

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GAGATGGAAGTTAATGTA-3'

Strain name: SYS653

Cross: OP620 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs620 (ceh-36::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-2, ZFH-2, CEH-6, UNC-30, DMD-4, PHA-2, TTX-1, EGL-38, PAX-1, COG-1, UNC-42, DMD-5, CND-1, CEH-27, CEH-48, EYG-1, ZAG-1, EGL-46, CEH-34, NGN-1, HLH-3, VAB-3


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ZFH-2, UNC-30, DMD-4, TTX-1, COG-1, DMD-5, CND-1, ZAG-1, EGL-46, NGN-1, VAB-3, AST-1, ATHP-1, BLMP-1, CEH-32, CEH-38, CEH-39, CEH-44, CEH-45, CEH-53, CEH-9, DAF-19, DSC-1, EFL-3, EGL-18, EGL-44, ELT-6, ETS-5, HLH-2, K09A11.1, LAG-1, LIM-7, LIN-22, LIN-31, LIN-39, LIN-48, NHR-102, NHR-25, NOB-1, POP-1, SPR-4, SYD-9, TBX-38, UNC-130, UNC-55, UNC-62, VAB-15, VAB-7, Y55F3AM.14, Y75B8A.6, ZIP-8, ZTF-6

Targeted by: ELT-1, ELT-2, ZIP-8, DSC-1, FKH-4, FKH-8, LSY-2, PAX-3, SOX-4

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ZFH-2, UNC-30, DMD-4, TTX-1, DMD-5, EGL-46, ATHP-1, CEH-39, CEH-45, CEH-9, DAF-19, DSC-1, EFL-3, EGL-44, ETS-5, HLH-2, LAG-1, LIN-22, LIN-31, LIN-48, NHR-25, NOB-1, POP-1, SYD-9, TBX-38, UNC-62, VAB-15, Y75B8A.6, ZTF-6

Targeted by: DSC-1, FKH-4, FKH-8, PAX-3, SOX-4