WormBase ID: WBGene00000429
WormBase description: Is an ortholog of human EMX1 (empty spiracles homeobox 1). Is predicted to have DNA-binding transcription repressor activity, RNA polymerase II-specific; RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding activity; and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Localizes to nucleus. Is expressed in neurons; pharynx; somatic nervous system; and vulva.
|
|
KI information: mNeonGreen knockin at N-terminus
sgRNA1: ATTTTCGGTGGAACGACTGG
sgRNA2: GGAATCGGAAGAAGCCGACG
5'HR: GCATGCGATTTGAAAACCCCAAAGAGTGACATTTGATATTCCACCTGCTGCTCTACACACTTCGCTCCTGTTGCTGTCGTCGGAGAGATGGCCGAGCGGTCCAAGGCGCTGGTTTAAGGCACCAGTCCCTTCGGGGGCGTGGGTTCGAATCCCACTCTCTTCAGTTTCTTTTTGAACAATTTTATAAATTTTGTGCTCATCTCGTGCTCACTTCACCTCGATTACTAACATAATATACCTATTTCTGAGCCCAACGTCCGCTGCTTTCGGGCGCACAAAAGATGTAATCCGAGATCTGCGATTTGTACATATTTCGGATACATTTTCAATCTCCATTTCCAACTCATCTTCTAATTCGGGACAATGAGCATCATCATGAGCATGTGTACACAAGGTAGTTCGGTCGCCCAGTTGCCGATTACATAGGCACCCGGTTGCCGAGTAGACGAGTTGTAAAAACAGGGCACCACGAGATATTGCCGATTTCCACATAAACTACTTATTAAAGTCTGGAGATTTAATTCATATGTCAAGAAAAATGTTCGTGAAAGCAACAATATATGTACTAGAAATCCCTAAGACGACGACTATCGCTGACGTTACATTGCTCCAAAAGAATGCAATGAATTGTGATTACACCATGAGAGTTTTCAAAACTTTAAACCTCAAATTAATATTTCAACTTATTAACCTACTTCCTATTCTTTTCAATTCTTTATTTGTCTATCGTCTCTTTATTCCCAATGATCTCTCTTCTAAGAGCTTCTACCTTATCGGCTACTGTGTCCAATTACCCATCGACGCTCCTCCCTCTTTTGCGCGAAACTGGGCGGAGCCTAAGATGAGATTCAGTAAAATAGGTTCTCATTTGGGAAATCAACTTACCCTTTTTGGCTAGTTTACTTATTTTTTCTAATATTCGGAGTGAAAGGTACC
3'HR: CTGCAGATGACTCTGAAATTTTCGGTGGAACGACTCGTAGATAGTGAGAAGGAATCGGAAGAAGCCGATGTAGAAGAGCAAAATAATTTGAAAAAAGTAAGTGAAAATTGGAATTATAAGATTTAAAAATCTTGTCTTTAGAGTTGAAAATGAGGTTCCAAAAAAATTGTGGGGAATTTAGCTCTCCGAAATTTTTTTTTCTTGTCTAATGTATAAGTTGTCCCTAGATAATTTTCTAGACTTTAAATCTGTAAATTATTGCATAGAAATGTTGGTTTTCAAAACTGTCCAAGATGAGTGAAAAAATACGAATTTTCAAATATAATAATTCAGTAAATTCCGTAGAGCTCAAATCCGCATTGGAGGCTACTTTTTTTAAACTCAAAGGCTGAAGCCTGCTTTTAGTCAGTTTCCTTTACATACCGTGACCTAGTTTCCACTTATTTCCTCTTTTACGAATTCCGTTTGTAAAGTTTTAAACGTACTTTTAAAATACAAATCAACTATTGAGCAGAATATTGCAAATTATTGCCCCATGAAAACAAACTCTTCCACCATGCTATTTTTTCAAATTTTTCAAAAAAAAAAACAAACTTAAATGTCAAATAAATCCCAGCTAGAAGAAGACGAAGAAGACGATGAACTATGTGAAAAATCAGGAAAGAATGAGCCACTTTCACAGACACTCTCATATTTTGACGTTCTTCTTCCGCATGTTCAAATGGCTTGTTCGAATCCGTTTATTTCGGGTATTGGAGCATCAGGCAGTGGAGATCAGAATTTGAATACAGGTGCTGGCGGATCTGTATGGCAACATCCATGGCTTGAATTGTTGCAAAGTACGACAGCTGCACAATTCGGTAAGATTATCAATCGAGTTTCCTAAAATAAATTATAAAGACTTTAAACTTGAGAAAATATAAAATTTTTACAGGTGACGTCACAGCTGGGCTCTTTCTTCAACCTCTCCGACTAGT
KI verification primers: CEH-2-NKIF: 5'-AATTACATAATCTCGATGTCGTCAGTG-3' CEH-2-NKIR: 5'-AACACTCACCTGTGTTTCCGTTAA-3' Download
|
Strain name: SYS606 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-2 (devKi178 [mNeonGreen::ceh-2]) I
|
Expression pattern: Pharynx-specific
|
Lineage expression pattern
350 stage
|
600 stage
 |
3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-16, CEH-45, Y48G1C.6, EYG-1, DMD-5, CEH-34, DVE-1, M03D4.4, CEH-27, PHA-4, CES-2, CEH-36, Y75B8A.6
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: DMD-5, PHA-4, CEH-38, CEH-39, CEH-44, CRH-2, DAF-12, DAF-19, EFL-3, EGRH-1, ETS-5, FOS-1, HBL-1, K09A11.1, LAG-1, LIM-7, MDL-1, NFI-1, NHR-102, RCOR-1, SDZ-38, SEL-8, SWSN-7, UNC-37, UNC-62, Y55F3AM.14, ZTF-6
Targeted by: CEH-34, PHA-4, BED-3, CEH-13, CEH-19, HND-1, LIN-32, LSY-2, PAX-1, SOX-4, SPTF-1, TBX-2, UNC-120 |
|
 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: DMD-5, CEH-39, CRH-2, DAF-19, EFL-3, ETS-5, LAG-1, MDL-1, NFI-1, RCOR-1, SWSN-7, UNC-37, UNC-62
Targeted by: CEH-34, BED-3, CEH-13, CEH-19, HND-1, LIN-32, LSY-2, SOX-4, SPTF-1, TBX-2, UNC-120
|