UNC-37

WormBase ID: WBGene00006773

WormBase description: Is an ortholog of human TLE3 (TLE family member 3, transcriptional corepressor) and TLE4 (TLE family member 4, transcriptional corepressor). Exhibits transcription factor binding activity. Is involved in several processes, including chemical synaptic transmission; regulation of axonogenesis; and regulation of synapse structure or activity. Localizes to nucleus. Is expressed in several structures, including germ line; head; and tail.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AAATTAAGCTTACTCGTTGC

sgRNA2: AAAAATGAAGGCATCGTATC UNC-37-NKIsg3: GCTCGTCTTTGATTCGATCG

5'HR: GCATGCGATTTCCTAATGAGTTTAAACTCTCGAGTTTTTATATGTGTAATATATTCTTCAACACTCCGGAATGCTTCTAATTAGTTACAAATGACAAAATGGAGCTTCATTCATGTTTTGGTTTTTTTTTAAATTTTCATGACTGCTACAGTATACCGTCTTGTTGTTTAATCTAATAGAATCATTCAAGAGTTTTTATTCTTTCCATTTGAATCTCAATTTTCACTATTATCATTTGATTCTCATCTCATTTTCTTACCGATTTTCAATCTCAAATTATTTAAGAACATACCATGTCAATAAACTTATTATTTGTTAAACATGATTTTTTAAAAATATTTTCTTCAAGACAGGTTTATGTCTCTTTATTCAATATTTCAACCTTTTCCGTTTTTTTAGATAATTTTGTGAGTTTTTTATACTGGAATTGTGATTATGTACATGTAAAATATTTATAAATGCTTCTCTCTATAGTAAGTTGCAGCTTCATCGTGTTTGATTCGAAATATTAGTTAGAAATCCTCGGATCCTCGTGTTACTTGAATATCAAACTTATTGGATACATTCATCTTCTACACTCGGGCTCAAAAGAGCTCAAAACAAATGTGCTCTATCGACAACTCCTCCCCCCATTCGGTCACCCATTCAGCGTCGCATTCATCGCCGCGCGTTCGTCCTCCTCGACACCAATACCTCCTTCATTCTTCAACGTTAGTGGGGCTATATGACCAGTCGTTTTGCAATTAATGTATGATTCTCTCCGTCTACACCACTGGAAACCCATGTCCTCTTTCTTTGTTTCTTCGTCTCGTTGTCAACGTCTATTCAAACCACGTGCTATTTCTTGAGTTTTGTTGTCTTCTCCCAATCCATCACTAGTCTGTTCTTCCTTGCATTTTTTTGATCGAATTTTTAAAACTCCTCTCATAATTTTTATGTTAATAATTCACTATCCACTTGATTGTATTTAATTTTCAGTAAAAGGTACC

3'HR: CTGCAGATGAAGGCATCGTATCTTGAAACTCTCGATCGAATCAAAGACGAGCACGCGGAAATGTCGAAGCATGTCAATCAGCAGCGAGTAAGCTTAATTTTGTAAGAGAAATTTTAGTATATTGAAAACAATTTTGCAGTCGGATATCGAAAAGGTTGCGTTAGAGAAAGAAAATATGAATAGATCGTATATGACGTACGCTGAAGTATCAAATACTCTTCGTAGCGATCTTCGAAAAGCAGAAGAAATCAACAAACGCCTTCAGGAATTCATCATGCAATCGGTAAATTAACTCGTCAATAATGTTATTAAAAAATCGAACAAAAATATAGAAAAAGCTATTACAATTTTTTTGTTGAAATATTTGAACTAAATATTATGGAAACGTTATAATATCTATTGGATTGTACCTTGTTCAACTCAAACATGCTCCTCTTTCCATATATTCATTTATAAATAGAAGAAAAATTATTATGTAATATTTTTAATTCATAATAATCAATTTTAGGTAATATTATAAATGTATATTTTTCAGTTGGCTCCTCAACTATCTCAAGATAATCAAGCAAACTGCCTCGCCGCACTGGAAGCTTTCAAAACCGCATCACCACGTGAAAATGGAAATGGAGCACCAGCACTTCCACCAGGTTTTCCTCCCGGTGCAGCTGGAATGCTTGGAATGATGCCAAATATGCCATTCGGAATGAGCCCTGCAATGAGTCAACTATTCAATCAATTCGCATCACCACATGTCAACGGTGGAGATGGAGCCGGTGGAAGTTCTGGAGGTGCAAGTGAAGCGAAAAAGGCCAAGCTGGAAGATCCAGATGATGGAGAACTTGAGATTGATGTCACAAATGATGATCATCCAAGTACTGCATCAAATGGAGGCGCAGCTAATAAAAATGGAAGAGATAGCACAAATAGTGTTGCCTCATCGGGAGCATCTACTAGT

KI verification primers: UNC-37-NKIF: 5'-GATCAGTTAGCAATGAAACGACAAT-3' UNC-37-NKIR: 5'-CTTAGCACACTTCAAAAGATTGTAAAC-3' UNC-37-NKIF2: 5'-TGAAATGATTGATCAAAGGGCATA-3' UNC-37-NKIR2: 5'-GAATCCTCCAGCAGCACTAGCT-3'

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Strain name: SYS681

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; unc-37 (devKi218 [mNeonGreen::unc-37]) I

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage



600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-39, CND-1, COG-1, DVE-1, ELT-2, FAX-1, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, BLMP-1, CEH-82, DPL-1, DSC-1, EFL-1, FKH-8, FOS-1, MDL-1, SKN-1, SWSN-7, UNC-86, VAB-15, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ALY-1, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-39, CND-1, COG-1, DVE-1, ELT-1, ELT-2, FAX-1, FKH-6, HLH-1, HLH-30, HMG-4, LIN-13, LSY-2, M03D4.4, MEP-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, BLMP-1, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, FKH-8, FOS-1, MDL-1, SKN-1, SWSN-7, UNC-86, VAB-15, ZTF-11