HLH-1

WormBase ID: WBGene00001948

WormBase description: Is an ortholog of several human genes including MYF5 (myogenic factor 5); MYF6 (myogenic factor 6); and MYOD1 (myogenic differentiation 1). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II regulatory region sequence-specific DNA binding activity; and protein homodimerization activity. Is involved in several processes, including positive regulation of mesodermal cell fate specification; positive regulation of transcription by RNA polymerase II; and transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including C lineage cell; D lineage cell; I4 neuron; MSaaaapa; and body wall muscle cell.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TTGGCTACTGTGCGTGTTGA-3'

Strain name: RW10481

Strain genotype: unc-119 (ed3) III; itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); stIs10436 (hlh-1::TGF(6.2B4)::GFP::TY1::3xFLAG)

Expression pattern: Lineage-specific, Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): SNAI-1, EGL-5, SYD-9, UNC-120, SEM-4, LIN-39, LSL-1, VAB-7, M03D4.4, HND-1, CEH-20, TBX-9, UNC-62, PAL-1, HMBX-1, CEH-40, TLP-1, GMEB-1, VAB-15, TBX-8, F23B12.7, CES-2, Y75B8A.6, CEBP-1, HBL-1, ATF-7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: SNAI-1, SYD-9, UNC-120, SEM-4, LIN-39, M03D4.4, CEH-20, UNC-62, TLP-1, VAB-15, CES-2, Y75B8A.6, CEBP-1, HBL-1, ALY-1, AST-1, ATFS-1, BCL-11, CAMT-1, CEH-13, CEH-14, CEH-18, CEH-34, CEH-38, CEH-39, CFI-1, CRH-2, DAF-19, DAF-3, DMD-5, DPFF-1, EFL-1, EFL-3, EGL-18, EGL-44, EGRH-1, ETS-5, FKH-2, HLH-11, HLH-30, LAG-1, LIM-7, LIN-1, LIN-15B, LSY-2, MBF-1, MDL-1, MEP-1, MML-1, NFI-1, NFYA-1, NHR-102, NHR-35, NHR-49, PHA-4, POP-1, RCOR-1, REF-1, RNT-1, SDZ-38, SEL-8, SPTF-1, SWSN-7, TTX-1, UNC-130, UNC-37, ZAG-1, ZTF-11, ZTF-6

Targeted by: UNC-120, M03D4.4, UNC-62, CEH-18, CEH-34, HMG-4, LSY-2, NFYA-1, ODD-2, RNT-1, SPTF-1, UNC-55, VAB-15, EFL-1, LIN-13, SWSN-7, TBX-2, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: SNAI-1, SYD-9, UNC-120, SEM-4, LIN-39, CEH-20, UNC-62, TLP-1, VAB-15, CES-2, CEBP-1, ALY-1, ATFS-1, CAMT-1, CEH-34, CEH-39, CRH-2, DAF-19, DAF-3, DMD-5, DPFF-1, EFL-1, EFL-3, EGL-44, ETS-5, FKH-2, HLH-30, LAG-1, LIN-15B, LSY-2, MDL-1, MEP-1, MML-1, NFI-1, NFYA-1, NHR-35, NHR-49, POP-1, RCOR-1, SWSN-7, UNC-130, UNC-37, ZAG-1

Targeted by: UNC-120, UNC-62, CEH-18, CEH-34, HMG-4, LSY-2, NFYA-1, ODD-2, RNT-1, SPTF-1, UNC-55, VAB-15, EFL-1, LIN-13, SWSN-7, TBX-2, UNC-130