TBX-2

WormBase ID: WBGene00006543

WormBase description: Is an ortholog of human TBX2 (T-box transcription factor 2) and TBX3 (T-box transcription factor 3). Exhibits enzyme binding activity. Is involved in several processes, including muscle cell fate specification; olfactory behavior; and pharyngeal muscle development. Localizes to cytoplasm and nucleus. Is expressed in amphid neurons; body wall musculature; head neurons; and pharynx.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AAACCGCTTCACTACCCACAC-3'

Strain name: SYS143

Cross: OP159 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs159 (tbx-2::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-22, PHA-2, UNC-86, TBX-7, DAF-19, DMD-4, CEH-34, HLH-14, EGL-46, DMD-5, Y48G1C.6, LIN-26, NHR-23, LIN-32, PHA-4, EGL-44, CES-2, CRH-2, M03D4.4, Y75B8A.6, ATF-7, HBL-1, F23B12.7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-22, DAF-19, DMD-5, NHR-23, PHA-4, CES-2, CRH-2, Y75B8A.6, HBL-1, AHR-1, ALY-1, ATFS-1, BCL-11, CAMT-1, CEBP-1, CEH-13, CEH-2, CEH-20, CEH-38, CEH-39, CEH-40, CEH-43, CEH-44, CEH-48, CFI-1, DAF-12, DAF-3, DUXL-1, EFL-1, EFL-3, EGL-18, EGRH-1, ETS-5, FOS-1, GMEB-1, HLH-11, HLH-30, K09A11.1, LAG-1, LIM-7, LIN-11, LIN-13, LIN-15B, LIN-22, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NHR-10, NHR-102, NHR-25, NHR-35, NHR-49, NHR-6, NOB-1, POP-1, RCOR-1, SDZ-38, SEL-8, SKN-1, SMA-2, SWSN-7, TAG-68, TLP-1, UNC-130, UNC-37, UNC-62, VAB-3, Y55F3AM.14, Y73E7A.1, ZAG-1, ZFH-2, ZTF-11, ZTF-6, PHA-2, EGL-46, LIN-32, EGL-44, M03D4.4, ATF-7, ARID-1, AST-1, ATHP-1, ELT-3, EOR-1, FAX-1, HLH-1, HMG-20, LIN-1, LIN-39, NHR-85, SEM-4, SYD-9, UNC-3, UNC-42, ZIP-3

Targeted by: CEH-34, PHA-4, ALY-1, BCL-11, CEH-18, CEH-31, ETS-4, FKH-6, HLH-30, LSY-2, NFYA-1, REF-2, SYD-9, UNC-120, ELT-2, MDL-1, RNT-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-22, DAF-19, DMD-5, NHR-23, PHA-4, CES-2, CRH-2, AHR-1, ALY-1, ATFS-1, CAMT-1, CEBP-1, CEH-13, CEH-2, CEH-20, CEH-39, CEH-40, CEH-43, CEH-44, DAF-3, DUXL-1, EFL-1, EFL-3, EGL-18, ETS-5, GMEB-1, HLH-30, LAG-1, LIN-13, LIN-15B, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NHR-10, NHR-35, NHR-49, NOB-1, POP-1, RCOR-1, SKN-1, SMA-2, SVH-5, SWSN-7, TLP-1, UNC-130, UNC-37, UNC-62, Y73E7A.1, ZAG-1, ZTF-11, PHA-2, EGL-46, LIN-32, EGL-44, M03D4.4, ATF-7, HBL-1, ALY-2, ARID-1, AST-1, ATHP-1, BCL-11, CEH-10, CEH-5, DPL-1, ELT-3, EOR-1, FAX-1, HLH-1, HLH-11, HMG-20, K10B3.5, LIN-39, NFYC-1, NHR-25, NHR-85, SEM-4, UNC-42, ZIP-3

Targeted by: CEH-34, PHA-4, ALY-1, BCL-11, CEH-18, CEH-31, ETS-4, FKH-6, HLH-30, LSY-2, NFYA-1, REF-2, SYD-9, UNC-120, ELT-2, MDL-1, RNT-1, UNC-130