WormBase ID: WBGene00017742
WormBase description: Is an ortholog of human NFYC (nuclear transcription factor Y subunit gamma). Is predicted to have RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is expressed in several structures, including excretory cell; gonad; head neurons; and oocyte.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TGAAGTTCTCTGGTGAAAGT
sgRNA2: ATTATTATAAAGATCAATTG
5'HR: GCATGCAATGCACAATATTTCACGACAGAAGAATAGTGTTGTTTTAATTTATTTTTTTCTAATTAGTTTAAAGTTAATTTTTCTTGCATCTGCCGTGATGTCGCAATTCCCAGAAGTTTTAGACAATAATGTAAGTAATACAATTTAAAATTTTCGACTTCCCAGCAAATGTTTCATTTATTTCAGCTTTTACACGCCGAAAACAACGAAACTGCCGAATACATACAAAATGATGGGACAAATCAATCAGAAGTGTTCATGGAACATCCCTACACGGTGAATAAAATCAACTCGTTTTAAAAACTTATTTATTTTATTTCATTTTAGCCGAACATGCATCCAATAAATATGCCTTCTATTGTAGAAGGTGCGGTTCATCCTTATAATAATTTAATCCATCACAATGATGCGATACCACCGGCAAAGTACGCCTCGATGAGACAAATGACAGAAGATTTCTGGCGTGAAAAAAAACAGAAAATGACTGAGATTTCTGAAGAGGATATGCTGAACAAGTCGAAAAACATGAGCGTTCCCATGGCTAGAGTGAAAAAGATTATGCGAATAGACGACGATGTTCGCAACTTTGTGAGTAGTGTCATTTTCCAGCCGATCTTCATACACTTTTTACAGATGATCGCGTCTGATGCTCCGATATTTATGGCTCAAGCAGCCGAGTTTTTCATTGAAGAAATGACCGCAATGGGCTGGCAATACGTGTCAGAAGCAAGGCGACGAATTCTTCAAAAAGCCGACATAGCAAGCGCAGTGCAAAAAAGTGATCAGTTCGATTTTCTCATCGACTTTCTACCTCCGAAAACGGTGCCAACAACTTCCACAAACGGCCCAGGCCATATGAGTGAAGACAGTTTCCAAGGTTTTCGGTTTTTTTCCTAAATATGATTTTTGTGCAATTTTTAACCTTTTATAGATCCCAACATGCATTCTGACTTTCATCAGAGAACTTCAAATTCTTCAGTCAACCGATCTCACCACAATGGTACC
3'HR: CTGCAGTCTTTATAATAATATTTTCTTGCTTATATTATGTATTAAAATTAAGTTCGTGGCGCCTGGCGCCCTTTAGTTCGTGGCGCCTGGCGCCCTTTAAAAGCTTGAATTTATACCTTTTTTGTCTAAAAATACTGTGTTTCATATGCACATTCATATATTTTCTTATTTCTTTGTTTTTCATTTTACAGGCTTTTACTTTGAAATCTTTAAATTTGAGGCGAAATGTGGTTGTCAAGTGAGGAGCTGAGTGCGAAGAAGACGGCTTGCCACGTGAGAAATGTGGAATCCAACAAAGAATTTGCAGATTCTGATATCGTTGCCTCATTCGTGCACAATCGGCCAAAAGATTTGAAAAACGTTTGTTCTAAAGCACCTTCTTTCAAATGAAAATAAAAATCCATTTTAGCTGACATTCGAAAGACACGGAAAAGGAGGACGTAAAAAAGCGCCAATATTCAGCGTAAAAGGAGATATTAGCGAGCATGTTGTAGAAGTTGGGGCAGATGTTAATTGTCTTGATGAGGGATTCGATTTTGCTGTTGCTTTGGTTGATAAGGTAATTTTGAGTGTATTTTAGCTAACAAATTCACTCTTTCAAAGGCTGAGTACCGAAACCTGAAAAATATTTTGGTACTACTTCTAAATCACCCCGCCACTTTTTTTCTTTTTTTTCAAATAAAAACGTGTGGAGTTTTTGTATTTTTAGGCTTAGGAAATAGCCATTTCTAAGCCTAAAGATGGAAAATGGACATCACGTTTTATTAAAATGAATAATATTAAAATGAGGGGAAATAATACACAAATAAAAAAAGTGGCGGGGTGATTTAGGACTACTACCAATATTTTTTAATGACTCCAAATTTTCCCCTGATTCCGAAAATCTATCAAAAAAAAATTTCACTGACTTTGGATTTCAGCTTGAAATCCCCGAATTTCATTTGTGAACCCTTGACTAGT
KI verification primers: NFYC-1-CKIF: 5'-AAAATCAGAATTGAAATTGTCATCG-3' NFYC-1-CKIR: 5'-CGCCATGTGTCGATTTACGA-3' NFYC-1-CKIF2: 5'-AGGTTCTCATGAGGAAAGAACGTC-3' NFYC-1-CKIR2: 5'-TTGTGCACCACCCATGAATAAC-3' Download
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Strain name: SYS678 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; nfyc-1 (devKi215 [nfyc-1::mNeonGreen]) II
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: PHA-4 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: LIN-13, LSY-2, MEP-1, NFYA-1, PHA-4, UNC-42, UNC-55, BLMP-1, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-8, FOS-1, IRX-1, MDL-1, RNT-1, SKN-1, SWSN-7, TBX-2, UNC-130, UNC-86, ZTF-11
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