IRX-1

WormBase ID: WBGene00007984

WormBase description: Is an ortholog of human IRX5 (iroquois homeobox 5). Is predicted to have DNA binding activity. Localizes to nucleus. Is expressed in several structures, including DD neuron; head muscle; head neurons; intestine; and somatic nervous system.

Reporter verification: Verified by whole-genome sequencing
Primers used to verify fosmid reporter: nan

Strain name: SYS84

Cross: OP536 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs536 (irx-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): ELT-3, DMD-4, CEH-60, LET-381, NHR-23, EGL-38, LIN-39, VAB-15, CEH-40, UNC-62, TBX-7, CEH-51, GMEB-1, CEH-20, LIN-26, NHR-25, BAZ-2, ELT-1, CEH-34, CEH-13, HBL-1, F23B12.7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ELT-3, VAB-15, UNC-62, NHR-25, ELT-1, CEH-13, HBL-1, ARID-1, AST-1, ATHP-1, CAMT-1, CEBP-1, CEH-38, COG-1, CRH-2, DAF-12, DAF-19, DAF-3, EFL-1, EGL-13, EGL-18, EGRH-1, ETS-5, FOS-1, HLH-11, HLH-30, K09A11.1, LAG-1, LIM-7, LIN-13, LIN-15B, MDL-1, MEF-2, MEP-1, MML-1, NHR-10, NHR-102, NHR-35, NHR-49, POP-1, RCOR-1, REF-2, SDZ-38, SKN-1, SMA-2, SWSN-7, TAG-68, TLP-1, Y55F3AM.14, ZTF-6

Targeted by: ELT-1, ALY-1, CEH-18, COG-1, ELT-2, NFYA-1, BLMP-1, DPL-1, DSC-1, LSY-2, MDL-1, MEP-1, NHR-85, RNT-1, SWSN-7

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ELT-3, VAB-15, UNC-62, NHR-25, ELT-1, CEH-13, HBL-1, ARID-1, AST-1, ATHP-1, CAMT-1, CEBP-1, CEH-5, COG-1, CRH-2, DAF-19, DAF-3, EFL-1, EGL-18, ETS-5, HLH-30, LAG-1, LIN-13, LIN-15B, MDL-1, MEF-2, MML-1, NFYC-1, NHR-10, NHR-35, NHR-49, POP-1, RCOR-1, REF-2, SKN-1, SMA-2, SWSN-7, TLP-1

Targeted by: ELT-1, ALY-1, CEH-18, COG-1, ELT-2, NFYA-1, BLMP-1, DPL-1, DSC-1, LSY-2, MDL-1, MEP-1, NHR-85, RNT-1, SWSN-7