WormBase ID: WBGene00044689
WormBase description: Is an ortholog of human ARID4A (AT-rich interaction domain 4A) and ARID4B (AT-rich interaction domain 4B). Is predicted to have DNA binding activity.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: ACTGGTTTTGGTCACGGTCT
sgRNA2: AGCTTGAGCTGCTGCTGCGG
5'HR: GCATGCGATCTTCCGGATTTTGATAAAATAATCGAGGAGGCTCCAAAAGAGGATATTAATATTCTGATGGAAGAGCGGACTTACGAGTTGCGAGAGATCTTTGCACAGTGTAAAGCTGATCTTTCGGCTCTTGAGAAGCGGTATAGGCAGCAGAATGAGGCGAAGCGTAAGTTTTTTTTTAGTAAGTAAAAGGAAATATTCGATTTTCGAGTGTTTTTTTTTGCGCGGAGAAATTTAAAACTCGAGAGTTTTTAAAAAAACAAGTTTCAAAAAACGAGAAAAGTATAGATTAGCCGAAAATATGTGGAATAAAATAGAAAATTTTAAAGCTGTTTTTTAAATTAAAATTTTCCGATAAATCTAATTTTTTGGTCTTTTTCTCAAAAAATTTAAATTTTTGAGCAACTATTGGAAAAGTCGATTTATTTTTGAAAAAGTTTTTTTTTCAGCGTTTGTATTTTATAAAATCGATATTTTTGAGCTATTTTTTTAAATTTTTTTGCACATTTATCGGAAATGATTTTTTGAAGGTTCTTTATCAAATACAACTCGAAAAACTCGGCGAAATTTGAGATTTTCACTAATTTGAGCTAATTTTTCAATGTTTTTTTGCCCTAAATAATTGTTAAAAAATTATAAAAAATTTAATGATTAATGATTCAATTTTGCAAAACAATATGTCCCTTTCAATTTTTTTCGTAAAATTGAAAAAAAAAATTTAGTTTGGGACGTCGATTTTCCGATAGTTTCTATTTCTTCTTATTTAAAAAATTGATTTTTCGAGTTACCGGAAAAGCAAAAAAAAAAACATTGAAAATTTTCCAAAAAAAAATCCAAAAACTTCTAATTTCCAGGAAAAGCTGAATTCGCCGCAAAAACTGCCTCTTCCGCAGCCGCAGCTCAAGCTTCTTCATCGACTTGCTCAACTCCAAGACCGGGTACC
3'HR: CTGCAGCCAAAACCAGTGGAATTTCTTAATGATTCTCTCTACTTATTCAACAAAACAAAAAATCTCAATTAAAATTGCATTTCCAATCAACATATGCCTTCCACACCCACGCATTTTCTCGATACTCTTCACCAGTCCCGCGGATAGAAATTTCTGTTCATTTTTTGTTTGTCTAGGATCTCTCTGTTATTACTGTGAATCAACTATGTGTGTGCTTTTTTTCTCCGTTTTAATTATGGGTGTCGTAGAAAATTTTTCGATTTTTGGGAAAATCAGATTTCAGATTTTTTGAGCTTTTCTCACGAAAAATCGAAATTTTTGAGCATTTTTTCGAAATTTTCAAAAAACGGAATTTTTCATGAAAAATCTAAATTCTTGAGTTATAGTTTTTGAACATTTGTTATCAAAAATTTGTTTTTTTTTTTTGGAAAACATCTAAATTTTTAAGTGTTTTCTCGAAAAATCGGAAAACCAAAAATTTTGATTTTCAAAAAAAAATCTAACAAATCAAGAGCTATTTTTCGAAAAATCGAAACAAAAGCGACACCCTTAGTTCTAATATATTCAATTTTTATTCTGATTCGATATATCTCCTTTAATTCCGTATTATAATTCCACAAACCTTCAGTTGTTCCCACCAAACTCTCAACTTATCTCAGTTCATTTCACTATAGCCGATTCTCGTAAACTTAATTTTTTTTTCGAATTTCCCCCAATTGCCTATGTTTTATTTAATGAGAAATTGATATAATAATGATGGTCTCAAATTTTGAAAAAAATATATTTATAGATTAACCGTAATTTTTATGTTCATGGCCTTTTTTGTGTTATGATTCGGGGAGTTTATCTAATGAAATTGTTTGAATTTTGAAGTTTTGAATATTTCAAAGTAGTAATGGGACTAGT
KI verification primers: ARID-1-CKIF: 5'-TTCAGACTCAGAAGAATCGAATGG-3' ARID-1-CKIR: 5'-TCTATTCTCCTCGTGGAGTACACG-3' ARID-1-CKIF2: 5'-AAACCTAAAGATCCTCATGCAGAAG-3' ARID-1-CKIR2: 5'-TGTGATGGAAAGCGCAAATTC-3' Download
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Strain name: SYS679 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; arid-1 (devKi216 [arid-1::mNeonGreen]) V
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern 4-cells 8-cells 15-cells 28-cells ~50-cells ~100-cells ~200-cells ~350-cells ~500-cells ~600-cells 600-cells bean-stage
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Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CEH-83, ELT-2, ELT-4, HAM-2, MEC-3, MEP-1, NFYA-1, PHA-4, RNT-1, CEH-82, DPL-1, EFL-1, ELT-3, IRX-1, LSY-2, MDL-1, SKN-1, SWSN-7, TBX-2 |
Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CEH-83, ELT-2, ELT-4, HAM-2, MEC-3, MEP-1, NFYA-1, PHA-4, RNT-1, CEH-82, DPL-1, EFL-1, ELT-3, IRX-1, LSY-2, MDL-1, SKN-1, SWSN-7, TBX-2
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