ARID-1

WormBase ID: WBGene00044689

WormBase description: Is an ortholog of human ARID4A (AT-rich interaction domain 4A) and ARID4B (AT-rich interaction domain 4B). Is predicted to have DNA binding activity.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: ACTGGTTTTGGTCACGGTCT

sgRNA2: AGCTTGAGCTGCTGCTGCGG

5'HR: GCATGCGATCTTCCGGATTTTGATAAAATAATCGAGGAGGCTCCAAAAGAGGATATTAATATTCTGATGGAAGAGCGGACTTACGAGTTGCGAGAGATCTTTGCACAGTGTAAAGCTGATCTTTCGGCTCTTGAGAAGCGGTATAGGCAGCAGAATGAGGCGAAGCGTAAGTTTTTTTTTAGTAAGTAAAAGGAAATATTCGATTTTCGAGTGTTTTTTTTTGCGCGGAGAAATTTAAAACTCGAGAGTTTTTAAAAAAACAAGTTTCAAAAAACGAGAAAAGTATAGATTAGCCGAAAATATGTGGAATAAAATAGAAAATTTTAAAGCTGTTTTTTAAATTAAAATTTTCCGATAAATCTAATTTTTTGGTCTTTTTCTCAAAAAATTTAAATTTTTGAGCAACTATTGGAAAAGTCGATTTATTTTTGAAAAAGTTTTTTTTTCAGCGTTTGTATTTTATAAAATCGATATTTTTGAGCTATTTTTTTAAATTTTTTTGCACATTTATCGGAAATGATTTTTTGAAGGTTCTTTATCAAATACAACTCGAAAAACTCGGCGAAATTTGAGATTTTCACTAATTTGAGCTAATTTTTCAATGTTTTTTTGCCCTAAATAATTGTTAAAAAATTATAAAAAATTTAATGATTAATGATTCAATTTTGCAAAACAATATGTCCCTTTCAATTTTTTTCGTAAAATTGAAAAAAAAAATTTAGTTTGGGACGTCGATTTTCCGATAGTTTCTATTTCTTCTTATTTAAAAAATTGATTTTTCGAGTTACCGGAAAAGCAAAAAAAAAAACATTGAAAATTTTCCAAAAAAAAATCCAAAAACTTCTAATTTCCAGGAAAAGCTGAATTCGCCGCAAAAACTGCCTCTTCCGCAGCCGCAGCTCAAGCTTCTTCATCGACTTGCTCAACTCCAAGACCGGGTACC

3'HR: CTGCAGCCAAAACCAGTGGAATTTCTTAATGATTCTCTCTACTTATTCAACAAAACAAAAAATCTCAATTAAAATTGCATTTCCAATCAACATATGCCTTCCACACCCACGCATTTTCTCGATACTCTTCACCAGTCCCGCGGATAGAAATTTCTGTTCATTTTTTGTTTGTCTAGGATCTCTCTGTTATTACTGTGAATCAACTATGTGTGTGCTTTTTTTCTCCGTTTTAATTATGGGTGTCGTAGAAAATTTTTCGATTTTTGGGAAAATCAGATTTCAGATTTTTTGAGCTTTTCTCACGAAAAATCGAAATTTTTGAGCATTTTTTCGAAATTTTCAAAAAACGGAATTTTTCATGAAAAATCTAAATTCTTGAGTTATAGTTTTTGAACATTTGTTATCAAAAATTTGTTTTTTTTTTTTGGAAAACATCTAAATTTTTAAGTGTTTTCTCGAAAAATCGGAAAACCAAAAATTTTGATTTTCAAAAAAAAATCTAACAAATCAAGAGCTATTTTTCGAAAAATCGAAACAAAAGCGACACCCTTAGTTCTAATATATTCAATTTTTATTCTGATTCGATATATCTCCTTTAATTCCGTATTATAATTCCACAAACCTTCAGTTGTTCCCACCAAACTCTCAACTTATCTCAGTTCATTTCACTATAGCCGATTCTCGTAAACTTAATTTTTTTTTCGAATTTCCCCCAATTGCCTATGTTTTATTTAATGAGAAATTGATATAATAATGATGGTCTCAAATTTTGAAAAAAATATATTTATAGATTAACCGTAATTTTTATGTTCATGGCCTTTTTTGTGTTATGATTCGGGGAGTTTATCTAATGAAATTGTTTGAATTTTGAAGTTTTGAATATTTCAAAGTAGTAATGGGACTAGT

KI verification primers: ARID-1-CKIF: 5'-TTCAGACTCAGAAGAATCGAATGG-3' ARID-1-CKIR: 5'-TCTATTCTCCTCGTGGAGTACACG-3' ARID-1-CKIF2: 5'-AAACCTAAAGATCCTCATGCAGAAG-3' ARID-1-CKIR2: 5'-TGTGATGGAAAGCGCAAATTC-3'

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Strain name: SYS679

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; arid-1 (devKi216 [arid-1::mNeonGreen]) V

Expression pattern: Non-specific

Lineage expression pattern

350 stage



600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-31, CEH-83, ELT-2, ELT-4, HAM-2, MEC-3, MEP-1, NFYA-1, PHA-4, RNT-1, CEH-82, DPL-1, EFL-1, ELT-3, IRX-1, LSY-2, MDL-1, SKN-1, SWSN-7, TBX-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-31, CEH-83, ELT-2, ELT-4, HAM-2, MEC-3, MEP-1, NFYA-1, PHA-4, RNT-1, CEH-82, DPL-1, EFL-1, ELT-3, IRX-1, LSY-2, MDL-1, SKN-1, SWSN-7, TBX-2