ELT-2

WormBase ID: WBGene00001250

WormBase description: Is an ortholog of human GATA4 (GATA binding protein 4) and GATA5 (GATA binding protein 5). Exhibits DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including defense response to other organism; endoderm development; and nematode larval development. Localizes to nucleus. Is expressed in several structures, including AWC; Ealaa; Ealp; Earaa; and intestine. Human ortholog(s) of this gene are implicated in several diseases, including Down syndrome; congenital heart disease (multiple); and hypoparathyroidism-deafness-renal disease syndrome.

KI information: mNeonGreen knockin at N-terminus

Reporter verification:Verified by comparing expression pattern to the literature (Fukushige et al., 1998)

Strain name: SYS412

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; elt-2 (dev99 [mNeonGreen::elt-2]) X

Expression pattern: Lineage-specific, Intestine-specific

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PQM-1, MML-1, MDL-1, NHR-232, ELT-7, END-1, NHR-102, END-3, ODD-2, NHR-12, DVE-1, DPFF-1, PHA-4, ATF-7, CEH-20, F23B12.7, CEH-74, LAG-1, SEL-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: PQM-1, MML-1, MDL-1, NHR-232, ELT-7, END-1, NHR-102, ODD-2, DVE-1, PHA-4, ATF-7, LAG-1, SEL-8, ALR-1, ALY-1, ARID-1, BCL-11, CEBP-1, CEH-16, CEH-36, CEH-38, CEH-39, CEH-40, CEH-60, CRH-2, DAF-12, DAF-19, DAF-3, DMD-5, DUXL-1, EFL-1, EFL-3, EGL-18, EGL-44, EGRH-1, ELT-3, ETS-4, ETS-5, FOS-1, GMEB-1, HBL-1, HLH-11, HLH-30, HMBX-1, IRX-1, K09A11.1, LIM-7, LIN-1, LIN-32, LIN-39, LIN-48, LSY-2, MBF-1, MEF-2, NFI-1, NGN-1, NHR-25, NHR-35, NHR-49, NHR-6, NOB-1, PAX-3, POP-1, RCOR-1, REF-1, SEM-2, SKN-1, SMA-2, SWSN-7, TBX-11, TLP-1, TTX-1, UNC-130, UNC-37, Y55F3AM.14, Y73E7A.1, Y75B8A.6, ZFH-2, ZIP-3, ZTF-6, ATFS-1, BLMP-1, CAMT-1, CEH-43, EGL-13, ETS-9, KLF-3, MEP-1, NHR-10, PHA-2, RNT-1, T18D3.7, TBX-2

Targeted by: PQM-1, LSY-2, PAX-3

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: PQM-1, MML-1, MDL-1, NHR-232, ELT-7, DVE-1, PHA-4, ATF-7, LAG-1, ALY-1, ARID-1, BCL-11, CEBP-1, CEH-16, CEH-39, CEH-40, CRH-2, DAF-19, DAF-3, DMD-5, DUXL-1, EFL-1, EFL-3, EGL-44, ELT-3, ETS-4, ETS-5, GMEB-1, HLH-11, HLH-30, IRX-1, K10B3.5, LIN-32, LIN-48, LSY-2, MEF-2, NFI-1, NGN-1, NHR-25, NHR-35, NHR-49, NHR-6, NOB-1, PAX-3, POP-1, RCOR-1, REF-1, SKN-1, SVH-5, SWSN-7, TBX-11, UNC-130, UNC-37, Y73E7A.1, ZFH-2, ZIP-3, ATFS-1, BLMP-1, CAMT-1, CEH-43, CEH-5, EGL-13, ETS-9, K09A11.1, KLF-3, NHR-10, PHA-2, RNT-1, SMA-2, T18D3.7, TBX-2, TLP-1

Targeted by: PQM-1, LSY-2, PAX-3