Single-Cell Protein Expression Atlas of Transcription Factors

CEH-20

WormBase description: Is an ortholog of human PBX2 (PBX homeobox 2). Exhibits RNA polymerase II transcription coactivator binding activity and proximal promoter sequence-specific DNA binding activity. Contributes to RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including mesodermal cell fate specification; positive regulation of mesodermal cell fate specification; and regulation of asymmetric cell division. Localizes to RNA polymerase II transcription factor complex. Is expressed in several structures, including P11.aaap; neurons; seam cell; somatic nervous system; and vulval cell.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GTCTAAAAGCTCGCTGAGAT

sgRNA2: GAAAATAAATGAGCAAACTT

5'HR: GCATGCGATTCAAAGCGCAAAATCCCTGCGTCTCCCTTCAATTGTTCAATGTCTAATAGTTCTTCTGGCAGATGATACGTCCTCACAACATCTGGCCTTTCCTTCCTTCCACTGCTACAAACACACATAGCAAAGGTCATCTCCTGATGTCTCTAGGTCTCTTCGAGTAAATGAAGAGGGTCTTTCTCTGTTGTTTCGAAGGAACCGCTACGCTGACACGCGACGCACGTCAAATGTCAACTTGTCTTCGTTCGACGTCTCCCCCCAACGTCGTTTTCGTGTATATCCATCTTTTTTCGAGACGTTTTTATTCCGTTTTTGCGGAAGGGTGTTGTTTTGAGTTATTGATCCCTTTGTTTTTCGACAAGAACCGTTTGATGTAAAAGTTAATATCAAGTGACAGATCCTGATGGATCCTGTCAGATTATGGTAGGATAGTATACTTTTTATAGTGAGTGAACTGACACTAGGTTGGCAAGAGTATACCTATCTACACATTTGCTCAAACAAAAATAACCTTACTACAATAATGGGAAGCGCTAGAAATTGTGCAACCACCGACCTAACACATCTTATTTTACGCACAATTTTGGCTCCATTACGGCCTGCCCAAATATTCGTGGTATAATAGAATCTATGTCTATTCCATACATTATGGCTATACTAAAATGTCGAAATAAGATTTTGAATTCTATTTTTTTAATCAAAAGTTATTTTACTATTCAGTTTTCACTTATCAAATGTTTATCATAATAAACTATCTTCAAATCTGACTTTCTTTGAAATTTCGATCCTACCACATGAGGCAATGTATTTTTGGGAAAATTCACACCAGATCTTCCGAACACCTACTAATTCAAGGTCATTTCCTCTTCTTTGTTTCTAAATTTCTCCTTCAAAACTTTCAGTTTTTAATTCAAATTTTTAACTTTTTTTTAGAATCTAATTATTAATTTCAGAAAGGTACC

3'HR: CTGCAGATGGTGGGGACACACCCAGCTAATCTTAGCGAGCTTTTAGACGCCGTCTTGAAAATAAATGAGCAGACTTTAGATGATAATGACAGTGCAAAGTGAGTTTTTTGTTTTTAGTTTTTGAGATTTGGTTTTTGAAAGAAATTCAGAAGACGAGCCATAATTTTTGGAGATCTAGAAAGTTGTTTTCAATTTTAGTAATAGTTTGTGAAAATATTCAAAAATGATAAAATTTTTGAGAACATCTCCACAGTATATTTTCCAAAACCTAAAATTTTATTTCTTTCAGAAAACAAGAGCTACAATGTCATCCCATGCGTCAAGCCCTATTCGATGTTCTCTGTGAAACAAAAGAAAAAACAGTACTGACAGTTCGTAATCAAGTGGACGAAACTCCAGAAGATCCTCAATTAATGCGACTTGATAATATGTTAGTGGCGGAAGGCGTTGCAGGACCAGATAAAGGAGGTTCATTAGGAAGTGATGCAAGTGGAGGTGATCAGGCTGATTATAGACAAAAGCTTCACCAGATTCGAGTTTTGTACAATGAAGAGCTGAGAAAGTATGAGGAGGTACGCTATTTTCAAAAGCAAAAACTCAGATCTGACTCAACATTGTTTAATTGAAATTTAGTTTTTTTTGTGAGTGTTCGCGAAAAAAAAGGTCCGAATAAAAACTAAAAATATTTTTTCAGGCTTGTAACGAATTCACGCAACACGTGAGGTCTCTGCTGAAGGATCAATCACAAGTTCGCCCAATAGCACATAAGGAAATCGAACGAATGGTTTATATAATCCAACGAAAATTCAATGGAATTCAAGTTCAACTCAAACAATCCACATGTGAGGCCGTCATGATCCTCAGAAGCAGGTTCCTTGATGCCCGCCGTAAACGGCGAAACTTTTCGAAACAAGCGACAGAAGTGCTTAATGAATATTTCTATGGACACCTCTCAAATCCATAACTAGT

KI verification primers: CEH-20-KIF: 5'-CAAAGCCGTAACAACAACGGTC-3' CEH-20-KIR: 5'-AATGATACCTGAGATACTGTAATATTGCAC-3'

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Strain name: SYS432

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-20 (devKi106 [mNeonGreen::ceh-20]) III

Expression pattern: Lineage-specific, Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): SNAI-1, LIN-39, SYD-9, RNT-1, CEH-40, LIN-22, EGL-5, ELT-3, UNC-62, PQM-1, NHR-23, GMEB-1, MDL-1, HLH-1, NHR-12, VAB-15, ELT-2, UNC-3, VAB-7, UNC-120, LIN-26, CEH-16, NHR-232, LIN-1, CEH-13, NHR-102, BLMP-1, TLP-1, HBL-1, M03D4.4, ELT-7, BAZ-2, END-1, ATF-7, PAL-1, PHP-3, IRX-1, F23B12.7, HAM-2, CEBP-1, TBX-9, Y75B8A.6, ELT-1, NHR-25, HMBX-1, CES-2, TBX-8

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, UNC-62, HLH-1, UNC-120, M03D4.4, HAM-2, ELT-1, TBX-8, CEH-18, CEH-31, CEH-34, CEH-39, CND-1, COG-1, HLH-30, HLH-8, HMG-4, LIN-13, LIN-32, LSY-2, MEP-1, MLS-2, NFYA-1, PHA-4, REF-2, SPTF-1, TBX-2, UNC-39, UNC-55, ZTF-11, ELT-3, MDL-1, BLMP-1, ALY-2, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, FOS-1, NHR-85, SKN-1, SWSN-7, UNC-130, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data