MLS-2

WormBase ID: WBGene00003377

WormBase description: Is an ortholog of human HMX1 (H6 family homeobox 1). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including establishment of mitotic spindle orientation; neuron differentiation involved in amphid sensory organ development; and regulation of mesodermal cell fate specification. Localizes to nucleus. Is expressed in several structures, including M.dla; M.dra; body wall musculature; head neurons; and somatic nervous system. Human ortholog(s) of this gene are implicated in oculoauricular syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GGGAGACCAGGGATTCCACT-3'

Strain name: SYS221

Cross: OP645 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs645 (mls-2::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, AHA-1, Y48G1C.6, CEH-16, DMD-5, AST-1, PAX-1, LIM-7, TTX-1, CEH-31, CEH-45, CEH-34, SEM-2, NGN-1, HLH-30, SOX-2, TBX-8, PHA-4, VAB-3, CEH-32, HAM-2, ELT-6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: DMD-5, AST-1, LIM-7, TTX-1, VAB-3, ELT-6, ATFS-1, CEBP-1, CEH-14, CEH-20, CEH-38, CEH-39, CEH-43, CEH-44, CES-2, CRH-2, DAF-19, DAF-3, EFL-3, EGL-18, EGL-44, EGL-46, ETS-5, GMEB-1, GMEB-2, HBL-1, HLH-11, HLH-2, K09A11.1, LAG-1, LIN-13, MDL-1, MML-1, NHR-102, NHR-23, POP-1, PROS-1, RCOR-1, SDZ-38, SEL-8, SEM-4, SPR-4, SWSN-7, SYD-9, UNC-37, UNC-62, VAB-7, ZAG-1, ZFH-2, ZTF-11, ZTF-6

Targeted by: CEH-31, CEH-43, REF-2, FKH-10, FKH-8, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: AHA-1, DMD-5, TTX-1, ATFS-1, CEBP-1, CEH-14, CEH-20, CEH-39, CEH-43, CES-2, CRH-2, DAF-19, DAF-3, EFL-3, EGL-44, ETS-5, GMEB-1, GMEB-2, HBL-1, HLH-2, LAG-1, LIN-13, MDL-1, MML-1, NHR-23, POP-1, PROS-1, RCOR-1, SEM-4, SVH-5, SWSN-7, SYD-9, UNC-37, UNC-62, ZAG-1, ZFH-2, ZTF-11

Targeted by: CEH-31, CEH-43, REF-2, FKH-10, FKH-8