WormBase ID: WBGene00006652
WormBase description: Is an ortholog of human OTX1 (orthodenticle homeobox 1). Exhibits DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including regulation of plasma membrane bounded cell projection assembly; thermosensory behavior; and transcription by RNA polymerase II. Localizes to nucleus. Is expressed in AFDL; AFDR; and marginal cell. Human ortholog(s) of this gene are implicated in several diseases, including Leri-Weill dyschondrosteosis; diabetes mellitus (multiple); and eye disease (multiple).
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: CTGTACTGAAAGTCGTACGC
sgRNA2: TCCGTCCAAAGTGTAGGTGT
5'HR: GCATGCAAAACATCATTTTCTATACAAACATGAGTTTTTATTTTGATGAATTTGAATTCAACACAAAATTTGAATTTTTTTTGACACCCATATTAACTATTTTCGGAATATTTTCAATTTTAGGCCATTTTAGGCCTGAAATCGGCCTGAAACGGCCTAAAAACCTCGAATTTTGGAACATATAAAAAATACGTGCACAACTGGTAGGAGTTTTGAGTTTCATGACCAAAAGTGTAAAAAGAATAGAAATTTCACATTTCAGGCCCGAAATCGGCCTGAAACGGCCTGAAATTTAATATTTCAAAACATAGTCAATATGAGTGTCAAAATTTTGCAAATTTTGTGTAGAATCTGAATTTTTCAATTTGGCAAGCCTTATGTGTCCCAAAAGTGTCCCAAATGGTGTCCCAAACGACACTCCGAAATTCCGCTCGGCTATTTTTCAGTGAAAACATCATTTTCTATACAAACATGAGTTATTATTTTGATGAGTTTGAATTCAACACAAAATTTGAATTTTTTGACACCCATATTAACTATTTTTCGAAAAATTTTCAGTTTTAGGCCTGAAATCGGCTTGAAACGGCCTGAAAATCTCGAGTTTTGGAAATCAACCAAAATACCTGTACAGTTTGTGAAAATTTAACAAGTAATTCAAGTTTATTGATCAAAAATGTTGAGTATCAAAAATTTCACGTTATAGGCCCGAAATCGGCTTGAATCGGCCTGAAATTTAAATATTTCAAAACCCAGTCAATATGGGTGTCAAAATTTTGCAGATTTTGTGTAGATCCCGAATTTTTTAGTCTTAAAAGTCCTAGATATCCCCAAATGTCCCAAACTTCAATTTTCAATCTTAAAAATCCTAATTTTCAGCCAGCTACCCTGCATATGATTTCCAGTACAGTCAATCCAACACAAATTCCACTAACACTTACACTTTGGACGGAAATTCCACTTGGAAATTTCAAATGAGCGGTACC
3'HR: CTGCAGAAAAAATCCGCGAATCTGTAAAAAAACCCACTAATTTTCACCTCTTTTTCACACAAATCTCTCCGGTTTTTAGTACGATGCTCCGATATTCCGGCGGAATTTCGGAGCATCGTACTGATCACAATTTTTCCTCTTTTTTCTACGCAAAAATCAATAATTTACGCACAAAAACTTCAAACTTCCATGATTTTTTCCCACTTCTTTTTCAATTTTTCTGACATTTTTAGGTCCCAAAGTCCTTTTTTCCCCTGAAAAATTTCAGTTTTTCACATTTCTAATATATCCTACGCATTCCAATATTATTGATTTACATATACTTTTACTATCAAGATTTTTCAATCAATAAATGTTCAAATTTGAAATTTATGTTTAAAAGTTCAAGTACCCAAACCTTTTTTTTTTGAATTTATAATCGCTTTTGACACAAAATTTGTTTAGAAATTCAGATTCTACACAAAATTTGCAAAGTTTTGACACCCATATTGGCTAGGTTAGCATTGCTTTGACTTTCAGGCCGATTCGGGCCGATTTCAAGCCGCAATAGGCTTTTTTGGGAATCTAGTCCAAATAGGCGTTCATTACATATACATTCATAAATGGTTCGATTATCTCATTTTTTGTCTTCAGAATAACTGTTACGGGGCCTTTTAGGCCTGAAATCGGCTTGAAACGGCCTGAAAAACTCAAGTTTCCAAACCTGGTCAATATGGGGTTTAAGATTTTGCAAATTTTGCGTAAAATCCAAATTTTTCATTTTCGAAAGTCCTAGCTAGCCCCAAGTGTCACAAACGACGCTCCGAAATTCCGCTCGATAAAGTTTAAAAATTATATGATTAAAAAAAAAAAGTTTGTTTAAATCACCGACCACCATGTTATTCAAACGTTTTCACGTACAATCTAATCATATTACTAGT
KI verification primers: TTX-1-CKIF: 5'-TCAACGCAAAGTTGTGTTTTTCT-3' TTX-1-CKIR: 5'-GGGGCACTTGAGGACAGCTA-3' Download
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Strain name: SYS644 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ttx-1 (devKi201 [ttx-1::mNeonGreen]) V
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Expression pattern: Lineage-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): PAX-1, ZFH-2, LIN-11, UNC-130, CEH-16, CEH-48, MLS-2, CEH-36, EGL-44, NGN-1, PHA-4, SPTF-1, HLH-3, M03D4.4, CES-2, CRH-2, CEBP-1, FKH-2
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: PAX-1, MLS-2, CEH-36, PHA-4, SPTF-1, CEH-83, CHE-1, COG-1, ELT-2, GMEB-2, HLH-1, LSY-2, NFYA-1, PHA-2, UNC-120, UNC-62, ZIP-8, BLMP-1, MDL-1, SKN-1, SWSN-7 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: PAX-1, MLS-2, CEH-36, PHA-4, SPTF-1, CEH-83, CHE-1, COG-1, GMEB-2, LSY-2, NFYA-1, PHA-2, UNC-120
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