NGN-1

WormBase ID: WBGene00003595

WormBase description: Is an ortholog of human NEUROG1 (neurogenin 1) and NEUROG3 (neurogenin 3). Is predicted to have protein dimerization activity. Is expressed in ABaraappaa. Human ortholog(s) of this gene are implicated in congenital malabsorptive diarrhea 4; diabetes mellitus (multiple); and persistent hyperplastic primary vitreous.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GCGGGTAGAATGGTGAATGA

sgRNA2: TGGCGAGCAAGATTTAGATA

5'HR: GCATGCAGGTGTCGTTGATCTCATTAAAAAAATTGCAAAACAATCATATAAAAAAAACCAATAAAATACCTATTATCTCTATTAAACTTCCTACATGCACCATGTGCACATTTGAATATATTTGAATTCAAAAATAGTTTAATTGAATTAGTTAGCTTATATCTCGATTTTCCCTGGTTATATCAACTAACAAAATACAAAGTATTATAAATGTTGCATTATTTTCAGCGATTAAAAAAATTAAAATTTTTTCTTAAGCCAAGATATACGCTGCCAAAGACATGGTACATCGAAAAATTTCGTTTCACTTAACTTTGATAGCTTGTATCAGGGGTGTGCGGCAAATTTGCCGAATTTGCTGAATTTGCCGTTTGCCGAGCTCGGCAAATTTGCCGAATTTGCTGAATTTGCCGTTTGTTAAGAATTCAGTAGTTTTGGTGCTCCAGAAAACATAAAAAATATCAAATTTTTCCGAGTTTGTTAAGCACGGCAAATTTGCCGAATTTGCCGAGCTCGGCAAATTTTGAGATTTGCCGCACACCCCTGGCTTGTATCTCGGTTATCTTTTTCGCTATCAAAAAGCTGCCAACTAACAAAATATTTGTTATTAAACGCTCTCCCCTATACAAGCTACCTGTCTTTCTGTATTTCCGTATAAGTGTCGTTATCTCCACTCCCACCCCACCAGTTTCTCACCTTTCAGAATGTACAGTCTTCCGGTTTGCACCTCTATTCCTCTTTTTCGGCGAGCTCTCCTACTCTGAAAAATCGCACCCCACCATATCTCCAGACTGGTGTTCCGGTTTGTTTCCCTTCTCTCTCTCTCTTTCTACCCCGCCCATCTCCTTACACCCCGCCCCCTTTATGTCCCATCTGTCATTAGTCAGTCATTTTCCACAGTGGTGCCCCCACCCACGTGTTTTTGTTTTGCACAGGTACC

3'HR: CTGCAGATGTATCATCACTCACCATTCTACCCGCATCATTTGCAAACTGGCGAGCAGGACTTGGACATGGAACGAGAAAATGATATGGATCAGGTTAGTTTTGGTTTTGGAAAAATGTTTAGAGAAATTTAAAACCAAATAATTTTTTTTTCGATTATTTTTTGAAAATTTCCGCAATTTTCTGAAGGTGCCAGATTTTTAAAGGTGGTGTTGTCGAGTTTTTTCATTGCTTCATTAGCCTTACAATTGTCTGAAAACGCCGAATTTCCTAATGAATCTTGAAAACTTCAAGTTTTAAACTATCGTAACTCCGTGGAGGTGGATGCTACAAAACATTTGTAAACTAACGTAATGTTGCAAATTTAATTTGTTTCATTTTATTAGTTAACAATTTTTGATAGGATGTATGGTTCCCGAGATATAAGCCTTCAAAATACTCATGGTATTGTACAGCTGTAACTTTCCTCGTGTAAATTTAAAACTAAAAAGTGGTAGAAAACTTCAAAATCTTCAATTTGTCAGTTGACAGTTTTTTAATAAATTTCCCGGTTAATGAGATATAAGCCTTTAAAGTTGTAGAATGATTTTTAGAAAACACCAAAATTAAAAATTGAATACATAGCTGTATCTAAACGGCGGTAATAGTTATGCAAAACTTGTCAACTGTCAAAATACTAATCAAACAATTTTCTACAAGTTGTTAGTTAACAACTTTTTAATAACTATTATGGCTAATAAGATATGCCCGTTTAAAGTTGTCTCAGCACACCCGAAAACTTTGAGTAGCTATATTTCGGCGGAAACAAAAACTGCTGAAAAATTGCTAACTAAAACATATTAAACAAAACATTTTTCTACATTTTGCTAATTAACAATCTTTAAATAGCTCTTGCAGAAATTTCAGTAAACTGTTTTTTTTCAGAAAAAAATGTTTAAAAAAAATTCATTCTCTATAACTTTGTTACTGTTCAAGCCAACTAGT

KI verification primers: NGN-1-NKIF: 5'-TGAGTGCATTTTTGTACTTGTCGG-3' NGN-1-NKIR: 5'-AAAGTCTTAGATTTTGGTACTCCTCCTT-3'

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Strain name: SYS498

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ngn-1 (devKi137 [mNeonGreen::ngn-1]) IV

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): AHA-1, FKH-10, CEH-17, LIM-4, UNC-42, EGL-13, EGL-46, CEH-10, CEH-48, ZAG-1, TAB-1, EGL-44, TTX-1, MLS-2, UNC-30, HLH-2, HLH-3, CEH-30, CEH-27, ZTF-11, VAB-3, HLH-30, ELT-6, CEH-36, SOX-2, CEH-32


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, ELT-2, LSY-2, UNC-62, ZIP-8, BLMP-1, DPL-1, EFL-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2