ZAG-1

WormBase ID: WBGene00006970

WormBase description: Is an ortholog of human ZEB1 (zinc finger E-box binding homeobox 1). Is predicted to have DNA binding activity and metal ion binding activity. Is involved in axon guidance and positive regulation of axon extension. Localizes to nucleus. Is expressed in body wall musculature; enteric muscle; neurons; pharynx; and somatic nervous system. Human ortholog(s) of this gene are implicated in Fuchs' endothelial dystrophy; Mowat-Wilson syndrome; and posterior polymorphous corneal dystrophy 3.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AACCACCGCTATTCGTACTGC-3'

Strain name: SYS102

Cross: OP83 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs83 (zag-1::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-17, UNC-3, UNC-42, TTX-3, CEH-14, CFI-1, LIN-11, CEH-48, EGL-38, EGL-46, CEH-6, NGN-1, CND-1, EGL-44, VAB-3, EYG-1, TAB-1, NHR-67, CEH-30, ATF-7, CEH-27, SOX-2, HLH-2, ELT-6, CEH-36, HLH-3, ZTF-11, HLH-30, CRH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-48, HLH-30, CAMT-1, CEBP-1, CEH-38, DAF-3, EFL-1, EGRH-1, ETS-5, HLH-11, LIM-7, MEF-2, NHR-49, RCOR-1, SDZ-38

Targeted by: UNC-42, CEH-14, CND-1, CEH-36, ZTF-11, ALY-1, BLMP-1, CEH-19, CEH-31, CEH-34, CEH-43, ELT-4, FKH-10, HLH-1, HLH-8, HMG-4, LIN-32, LSY-2, MLS-2, NFYA-1, RNT-1, SOX-4, SPTF-1, TBX-2, TBX-8, UNC-120, UNC-55, UNC-62, ZIP-8, CEH-8, DSC-1, FKH-4, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-48, HLH-30, CAMT-1, CEBP-1, CEH-5, DAF-3, EFL-1, ETS-5, MEF-2, NHR-49, RCOR-1

Targeted by: UNC-42, ALY-1, BLMP-1, CEH-31, HLH-1, HLH-8, HMG-4, LSY-2, MLS-2, NFYA-1, RNT-1, SOX-4, SPTF-1, TBX-2, UNC-120, UNC-55, UNC-62, DSC-1, FKH-4