HLH-8

WormBase ID: WBGene00001953

WormBase description: Is an ortholog of human TWIST1 (twist family bHLH transcription factor 1) and TWIST2 (twist family bHLH transcription factor 2). Is predicted to have DNA binding activity and protein dimerization activity. Is involved in mesodermal cell fate specification; positive regulation of transcription by RNA polymerase II; and vulval cell fate specification. Is expressed in several structures, including M.dla; M.dra; M.vla; body wall muscle cell from M lineage; and enteric muscle. Human ortholog(s) of this gene are implicated in several diseases, including Barber-Say syndrome; Sweeney-Cox syndrome; and synostosis (multiple).

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AGCCTTGGTATCAGACTTGTCC-3'

Strain name: SYS160

Cross: OP74 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs74 (hlh-8::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): DSC-1, SNAI-1, LET-381, UNC-120, PAL-1, M03D4.4, F23B12.7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, CEH-20, CEH-38, CRH-2, DAF-19, EGL-18, EGRH-1, ETS-5, HBL-1, HLH-11, HLH-2, K09A11.1, LIM-7, MDL-1, MLS-1, NFI-1, NHR-102, POP-1, RCOR-1, SWSN-7, TAG-68, UNC-62, ZAG-1, ZTF-6

Targeted by: DSC-1, FKH-4

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, CEH-20, CRH-2, DAF-19, ETS-5, MLS-1, NFI-1, POP-1, RCOR-1, SWSN-7, UNC-62, ZAG-1

Targeted by: DSC-1, FKH-4